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Method for adjusting biological membrane surface topological structure to promote cell crawling

A topology and biofilm technology, applied in prosthetics, coatings, medical science, etc., can solve problems such as the influence of cell crawling on the surface pore size of biofilm materials, and achieve low raw material cost, simple operation process, and promote The effect of growth

Inactive Publication Date: 2017-06-06
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

They also did not explore the effects of changes in the surface pore size, pore gap, and nano-protrusions of biofilm materials on cell crawling, nor did they consciously regulate the pores and surface nanostructures of nanofibrous membranes.

Method used

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  • Method for adjusting biological membrane surface topological structure to promote cell crawling
  • Method for adjusting biological membrane surface topological structure to promote cell crawling
  • Method for adjusting biological membrane surface topological structure to promote cell crawling

Examples

Experimental program
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Effect test

Embodiment 1

[0024] (1) Prepare aqueous solutions of lysozyme and type I-collagen respectively, with a concentration of 1 mg / mL, and stir until the solute is completely dissolved; mix the above two solutions in a ratio of 1:1 by volume to obtain a mixed solution of lysozyme and collagen. And use glacial acetic acid and ammonia to adjust its pH to 6.3.

[0025] (2) Prepare an aqueous solution of silk fibroin with a concentration of 1 mg / mL, and adjust its pH to 8.0 with glacial acetic acid and ammonia.

[0026] (3) The silk fibroin membrane provided by Hubei Sailuo Biotechnology Co., Ltd. is used as the bottom plate membrane. Soak the silk fibroin membrane in the lysozyme-silk fibroin mixed solution for 20 minutes, and then wash it with ultrapure water three times for 3 minutes each time. So far, it is considered that 0.5 bilayers have been assembled; then in the silk fibroin solution Soak for 20 minutes, and wash with ultrapure water three times. So far, it is considered that a bilayer has bee...

Embodiment 2

[0033] (1) Use 0.5% NaCO 3 The solution degummed the silkworm silk, dissolved it with a calcium chloride / ethanol / water ternary solution after scouring, dialysis, purification, and concentration to obtain a 2.5-3.5% silk fibroin solution, which was freeze-dried to obtain a silk fibroin membrane.

[0034] (2) Cross-link the obtained silk fibroin membrane with 75% ethanol for 1 hour, dry at room temperature and then further vacuum dry.

[0035] (3) Prepare chitosan solution (1mg / mL) with 2% acetic acid, and adjust the pH to 5.0 after magnetic stirring until it is completely dissolved; prepare I-type collagen aqueous solution (1mg / mL), magnetically stir until it is completely dissolved, then use ice Acetic acid and ammonia water adjust the pH to 5.0.

[0036] (4) First, the chitosan solution is assembled on the surface of the silk fibroin / polycaprolactone composite nanofiber membrane obtained in step (2) by spin coating, and it is naturally dried. The spin coating parameters are: 0.3mL,...

Embodiment 3

[0041] (1) Using electrospinning technology to prepare 7wt% silk fibroin / polycaprolactone composite nanofiber membrane, in which the mass ratio of silk fibroin to polycaprolactone is 5:1. The electrospinning parameters include: the perfusion rate is 1.0mL / h, the voltage of the high-voltage DC power supply is 16kV, the distance from the needle to the receiving plate is 15cm, the temperature is 27℃, and the humidity is 50%.

[0042] (2) Cross-link the obtained silk fibroin / polycaprolactone composite nanofiber membrane with 75% ethanol for 1 hour, dry at room temperature and then further vacuum dry.

[0043] (3) Prepare chitosan solution (1mg / mL) with 2% acetic acid, stir magnetically until it is completely dissolved, adjust the pH to 5.0 with glacial acetic acid and ammonia water; prepare I-type collagen aqueous solution (1mg / mL), stir slowly with magnetic force After it is completely dissolved, adjust the pH to 5.0 with glacial acetic acid and ammonia water.

[0044] (4) The silk fib...

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Abstract

The invention discloses a method for adjusting a biological membrane surface topological structure to promote cell crawling and belongs to the technical field of macromolecular biological medical materials. The method disclosed by the invention utilizes polyelectrolyte to self assemble target biological membranes layer by layer and adjust surface pore sizes, the hole intervals and the surface nano-protrusions of the biological membranes and comprises the specific following steps of choosing two kinds polyelectrolyte with opposite charges, choosing the polyelectrolyte with charges opposite to the surface charges of the target biological membranes to self assemble according to surface charges of the target biological membrane, then assembling the other kind of polyelectrolyte with the opposite charges, assembling a double-molecular layer and repeating until a required layer amount is obtained. According to the method disclosed by the invention, the layer-by-layer self-assembling technology is applied to adjusting the surface pore sizes and the nano-protrusions of the biological membrane material and giving extra performance to the biological membrane material, so that biological medical value can be maximum; the method has the advantages of low raw material cost, a simple operation technology, moderate conditions and the like and is suitable for large-scale production.

Description

Technical field [0001] The invention belongs to the technical field of polymer biomedical materials, and specifically relates to a method for regulating the surface topological structure of a biofilm to promote cell crawling. Background technique [0002] The medical value of biomembrane materials is not only reflected in the good biocompatibility of the raw materials themselves, but also closely related to the surface topology. As membrane materials for cell growth scaffolds, in order to better mimic the extracellular matrix, they need to have a suitable pore size and high porosity. Since the effect of cells and extracellular matrix is ​​at the nanometer level, there are more and more studies on the nanometer-level protrusions on the surface of materials and their effects on cell adhesion, crawling and differentiation. Since tissue repair is a process in which new cells crawl to replace necrotic tissue, only porous scaffolds with appropriate pore sizes, voids and nano-protrusio...

Claims

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Application Information

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IPC IPC(8): A61L27/44A61L27/54A61L27/56
CPCA61L27/34A61L27/54A61L27/56A61L2300/252A61L2300/404A61L2300/414A61L2300/416
Inventor 邓红兵武郭敏施晓文杜予民
Owner WUHAN UNIV
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