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Preparation method of almond-polypeptide-containing skin care products

A technology of almond and deionized water, which is applied in the preparation method of peptide, skin care preparation, cosmetic preparation, etc., can solve the problem that almond peptide has not been used in cosmetics, etc., and achieve good water solubility, strong anti-oxidation, quality stable effect

Inactive Publication Date: 2017-06-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to literature reports, almond polypeptide has the effects of scavenging free radicals, lowering blood sugar, and inhibiting bacteria, but so far, almond polypeptide has not been used in cosmetics

Method used

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  • Preparation method of almond-polypeptide-containing skin care products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1 Preparation of almond polypeptide

[0013] The almonds are vacuum dried and crushed, the remaining fat is removed with n-hexane, and crushed and sieved. Using alkaline extraction and acid precipitation method, add deionized water according to the material-liquid ratio of 1:30g / mL, add NaOH to adjust the pH to 9.5, stir for 2h at 45℃, adjust the pH of the centrifugal supernatant to the protein isoelectric point of 3.4, and centrifuge to collect the precipitate . Wash the precipitate 4 times and heat for 30 minutes to fully denature the protein. The pellet after centrifugation is freeze-dried to obtain powdered almond protein.

[0014] The almond protein was added with alkaline protease 2-3% (g / g) according to the substrate concentration of 5%, and the pH was kept constant at 9.5 at 55° C. and hydrolyzed for 3 hours. Heat to 95°C to inactivate the enzyme for 20 minutes, centrifuge to take the supernatant and freeze-dry to obtain powdered almond crude polypeptide. ...

Embodiment 2

[0019] Example 2 Mild moisturizing skin care cream, based on the total amount of 1000 g.

[0020] Aqueous phase: glycerin 50g, deionized water 460.5g, sodium hydroxide 0.5g.

[0021] Oil phase: sorbitan trioleate 20g, sorbitan tristearate 15g, cetearyl alcohol 50g, squalane 100g, caprylic / capric triglyceride 40g, jojoba oil 20g, 30g glyceryl monostearate, 80g polydimethylsiloxane, 10g retinol, 60g octyldodecanol, 5g phenoxyethanol.

[0022] Post-addition: 15g of acrylic (ester) copolymer, 10g of panthenol, 30g of almond peptide, 2g of octylene glycol, 2g of flavor.

[0023] Production process: Add the oil phase raw materials into the main emulsification pot and heat and stir to 85±2°C. After they are fully dissolved, add the water phase raw materials into the auxiliary emulsification pot and heat to 60±2°C. Slowly add the water phase to the oil phase, homogenize at 3000rpm for 20min, cool to 45±2℃, keep stirring for 30min, add the phase after adding, homogenize at 2000rpm for 15min, ...

Embodiment 3

[0026] Example 3 Anti-aging emulsion, based on the total amount of 1000 g.

[0027] Pre-mixed phase: 25 g of 3% hydroxyethyl cellulose aqueous solution.

[0028] Water phase: 621.8g deionized water, 80g glycerin, 30g butanediol.

[0029] Oil phase: cetearyl ether-212g, cetearyl ether -2115g, hydrogenated polyisobutylene 50g, polydimethylsiloxane 30g, caprylic / capric triglyceride 30g, ethylhexyl palmitate 40g, 5g of butylmethoxydibenzoylmethane, 5g of 4-methylbenzylidene camphor, and 5g of phenoxyethanol.

[0030] Post-addition: 30g of almond peptide, 1g of triethanolamine, 5g of vitamin E acetate, 5g of nicotinamide, 10g of water-soluble azone / PEG-40 hydrogenated castor oil, 0.2g of essence.

[0031] Production process: Mix hydroxyethyl cellulose with deionized water into a 3% aqueous solution, and disperse it with a dispersing disc for 1 hour to obtain a premixed phase. Add the pre-mixed phase to the water phase, heat and stir in the main emulsification pot to 80-83°C, and heat and s...

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Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to an almond-polypeptide-containing mild moisturizing skin cream, antiaging lotion and eye cream, almond polypeptides, and a preparation method of the skin care products. The almond polypeptides are prepared by the following steps: degreasing almonds, extracting proteins by alkali extraction and acid precipitation, carrying out enzymolysis by alkaline proteinase, desalting through a DA-201C macroporous adsorbent resin, eluting with a 75% ethanol-water solution, and carrying out freeze-drying. The almond polypeptides have abundant varieties of amino acids, and thus, have the antioxidation activity. The mild moisturizing skin cream contains 0.2-3 wt% of the almond polypeptides. The antiaging lotion contains 0.5-5 wt% of the almond polypeptides. The eye cream contains 1-5 wt% of the almond polypeptides. The almonds provide economical high-added-value application. The sensory evaluation result indicates that the prepared skin cream, lotion and eye cream have the skin care effect.

Description

Technical field [0001] The invention relates to the field of cosmetics, in particular to a skin care product containing almond polypeptide and a preparation method and application thereof. Background technique [0002] As people pay more and more attention to the efficacy of skin care products, natural active ingredients in skin care formulations have become a research hotspot. The natural product series of skin care products have a broad market prospect because of their functional ingredients derived from plants and high safety. In recent years, major cosmetics brands have launched peptide-containing products, such as Xiangyi Materia Medica's Quadruple Extraction Silk Series which contains silk peptides, Estee Lauder Aquamarine Mystery contains yeast peptides, and Olay Multi-effect Repair Series contains wheat hydrolyzed protein peptides. [0003] Almonds are rich in oil, protein and other nutrients, so almond oil and almond protein have been widely used in food and cosmetics. A...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/16A61K8/64A61Q19/00A61Q19/08
CPCA61K8/645A61Q19/00A61Q19/005A61Q19/08C07K1/16C12P21/06
Inventor 孙培冬陆帅宁奇曹光群
Owner JIANGNAN UNIV
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