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Nanometering method for improving intestinal absorption of hericium erinaceus polysaccharide

A technology of Hericium erinaceus polysaccharide and intestinal absorption, applied in the field of medicine, can solve the problems of reducing the encapsulation rate, being unfavorable to the formation of small nanoparticles, complex substances in the Hericium erinaceus extract, and achieving the effect of improving the efficacy of the medicine

Inactive Publication Date: 2017-06-13
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the nanometerization method of Hericium erinaceus polysaccharides includes PLGA solvent diffusion method, but there are three problems: 1. Directly mixing polysaccharides with PLGA is not conducive to the encapsulation of polysaccharides, which reduces the encapsulation efficiency
2. Using dichloromethane as the organic phase is not conducive to the formation of smaller nanoparticles
3. Adding the suspension dropwise to the aqueous gelatin solution can easily cause leakage of water-soluble drugs from the polymer phase
4. Hericium erinaceus extract contains complex substances, which is not conducive to the determination of active ingredients and the improvement of drug efficacy

Method used

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Embodiment 1

[0016] A nanometerization method for improving intestinal absorption of Hericium erinaceus polysaccharides, said method comprising the following steps:

[0017] (1) Make a 5wt.% solution of purified Hericium erinaceus polysaccharide and put it in a beaker, then add 5wt.% PEG solution according to the volume ratio of Hericium erinaceus polysaccharide solution: PEG solution is 1:40, magnetic stirrer Stir at 2000 rpm for 10 minutes, let it stand until the bubbles on the surface disappear, and then pre-freeze in a refrigerator at -20°C for 12 hours;

[0018] (2) Freeze the frozen sample in a vacuum freeze dryer for 24 h; wash the freeze-dried powder with dichloromethane, vortex to dissolve the PEG fully, then centrifuge at 12000 rpm for 5 min, remove the supernatant, and add New dichloromethane, repeated 3 times, put into a vacuum oven overnight to obtain dry polysaccharide nanoparticles;

[0019] (3) Dissolve polysaccharide nanoparticles and PLGA in a mixture of dichloromethane ...

Embodiment 2

[0022] 1. Preparation of Chicken Intestinal Epithelial Primary Cells

[0023] Aseptically take intestinal tissue from chicken embryos, wash repeatedly with PBS, remove mesentery, and cut into smaller than 1 mm 3 The tissue blocks were digested with type Ⅰ dispase and type Ⅺ collagenase, and stirred in a 37°C water bath at 80 r / min for 30 min. After the digestion was completed, centrifuge at 1000 r / min for 10 min, add a small amount of DMEM medium to the cell pellet to suspend the cells, centrifuge and wash 3 times, and wash the collected cells with 10% FBS-containing cell culture medium DMEM 12 g / L + 20 μg / L epidermal growth factor+100 mg / L heparin sodium+110mg / L sodium pyruvate+2.5 mg / L insulin+200 mmol / L glutamate+100 U / L penicillin+100 μg / L streptomycin suspension, Planted into glass cell flasks.

[0024] 2. Establishment of Chicken Small Intestinal Epithelial Cell Monolayer Model

[0025] Cells in the logarithmic growth phase were divided into 1 × 10 5 / cm 2 Culture...

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Abstract

The invention provides a nanometering method for improving intestinal absorption of hericium erinaceus polysaccharide. The nanometering method comprises: mixing purified hericium erinaceus polysaccharide and PEG (polyethylene glycol) solution, and pre-freezing for 12 hours; placing a frozen sample into a vacuum freezing drying machine to freeze-dry for 24h; washing freeze-dried powder with dichloromethane, and after drying, obtaining polysaccharide nano particles; dissolving the polysaccharide nano particles and PLGA into mixed solution of dichloromethane and acetone, carrying out ultrasonic processing to obtain suspension, adding the suspension into aqueous solution of pluronic, which has concentration of 5%, placing the solution into a rotary evaporator, and until the dichloromethane and the acetone are volatilized completely, carrying out centrifugation and microsphere sedimentation separation-out; collecting settled microspheres, washing the microspheres with distilled water, and carrying out vacuum freeze-drying to obtain a finished product. According to the nanometering method provided by the invention, a nano diffusion method is adopted for the first time to coat the purified polysaccharide, medicine composition is cleared, and medicine efficacy is improved.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a nanometerization method for improving intestinal absorption of Hericium erinaceus polysaccharide. Background technique [0002] Hericium erinaceus polysaccharide is an effective active ingredient extracted from the high-quality Hericium erinaceus fruiting body. The main components of Hericium erinaceus are polysaccharides and erinacein. Ehrlich ascites cancer has obvious inhibitory effect, and at the same time has the functions of helping digestion and benefiting the five internal organs. It has a good curative effect on chronic gastritis, duodenal ulcer and other digestive tract diseases, and can improve human immunity. [0003] At present, the nanometerization method of Hericium erinaceus polysaccharide includes PLGA solvent diffusion method, but there are three problems: 1. Direct mixing of polysaccharide and PLGA is not conducive to the encapsulation of polysac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61K47/34A61K47/10A61K47/14A61P35/00A61P1/04A61P37/04A61K36/07
CPCA61K31/715A61K9/0053A61K36/07A61K47/10A61K47/14A61K47/34
Inventor 秦韬黄一帆李健马玉芳黄小红俞道进任喆
Owner FUJIAN AGRI & FORESTRY UNIV
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