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Treatment process of waste cells from amino acid fermentation

A treatment process and technology of waste bacteria, which is applied in the field of treatment process of waste bacterial protein fermented by threonine, can solve the problems of deep color of hydrolyzed liquid, difficult removal of impurities, low product purity, etc., to achieve large particle size and improve purity , The effect of simple preparation process

Active Publication Date: 2021-04-27
内蒙古阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparation of oligopeptides by proteolysis mainly includes enzymatic hydrolysis and acid hydrolysis. Both have their own advantages and disadvantages. Enzymatic hydrolysis is relatively mild, with few impurities and high purity, and relatively high cost; acid hydrolysis is relatively low in cost, but the color of the hydrolyzed solution Deeper, difficult to remove impurities, lower product purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A treatment process for amino acid fermentation waste cells, which comprises the following steps:

[0027] Step 1) Drying and crushing: Dry the waste cells from amino acid fermentation at 80°C, and then crush them into cell powder with a grinder;

[0028] Step 2) Salt treatment and ultrasonic treatment: Add the same mass of 5wt% sodium chloride aqueous solution to the bacterial powder, adjust the pH to 2 with sulfuric acid, and use 20kHz ultrasonic treatment for 20 minutes;

[0029] Step 3) Hydrolysis: Put the material obtained in step 2) in the reactor, control the pressure in the reactor to 0.8MPa, the temperature to 100°C, keep the heat for 8 minutes, then reduce the pressure to normal pressure, maintain the temperature at 100°C, continue Sulfuric acid was added to adjust the pH to 1, and the hydrolysis time was 4 hours to obtain a hydrolyzed solution, which was naturally cooled to room temperature, then centrifuged at 500 rpm for 3 minutes, and the supernatant and p...

Embodiment 2

[0037] A treatment process for amino acid fermentation waste cells, which comprises the following steps:

[0038] Step 1) Drying and crushing: Dry the waste cells from amino acid fermentation at 80°C, and then crush them into cell powder with a grinder;

[0039] Step 2) Salt treatment and ultrasonic treatment: Add the same mass of 5wt% sodium chloride aqueous solution to the bacterial powder, adjust the pH to 2 with sulfuric acid, and use 20kHz ultrasonic treatment for 30 minutes;

[0040] Step 3) Hydrolysis: Put the material obtained in step 2) in the reactor, control the pressure in the reactor to 1MPa, the temperature to 120°C, keep the heat for 8 minutes, then reduce the pressure to normal pressure, maintain the temperature at 120°C, and continue to add Sulfuric acid, adjust the pH to 1, and the hydrolysis time is 2 hours to obtain the hydrolyzed solution, naturally cool to room temperature, then centrifuge at 500rpm for 3min, and collect the supernatant and precipitate; ...

Embodiment 3

[0048] Taking Brevibacterium flavum for the fermentation of threonine as an example, the content of each component is (measured by dry weight); crude protein 71-73%, nucleic acid 6.2-6.8%, crude fat 12-14%; the balance is other.

[0049] The degree of hydrolysis of the obtained hydrolyzate after hydrolysis is detected:

[0050] The embodiment 1 of the present invention is used as the experimental group; the control group 1 adopts the conventional enzymolysis method in this field: the addition ratio of acid protease and beta-glucanase is 2:1, the total enzyme amount is 2%, and the pH value is 4.0 , the hydrolysis temperature was 50°C, the substrate concentration was 15%, and the hydrolysis time was 12h; the acid hydrolysis method was adopted for control group 2: adjust the pH to 1 with 6mol / L hydrochloric acid, and hydrolyze at 110°C for 20h.

[0051] Determination of total nitrogen by Kjeldahl method; determination of amino acid nitrogen by formaldehyde titration; calculation ...

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Abstract

The invention belongs to the technical field of amino acid production and discloses a treatment process for amino acid fermentation waste cells, which includes the following steps: step 1) drying and crushing, step 2) salt treatment, ultrasonic treatment, step 3) hydrolysis, and step 4) adsorption , centrifugation, step 5) filtration, step 6) evaporation and drying, step 7) preparation of feed. This busy treatment process can prepare high-purity peptide products and feed products, avoiding the waste of bacterial raw materials.

Description

technical field [0001] The invention belongs to the technical field of amino acid production, and relates to a treatment process for amino acid fermentation waste cells, in particular to a treatment process for threonine fermentation waste cell proteins. Background technique [0002] Fufeng Group is mainly committed to the production, operation and research and development of bio-fermentation products. It is the world's largest producer of monosodium glutamate and glutamic acid, the world's third largest producer of xanthan gum, and the world's largest producer of threonine, leucine and histidine. A major manufacturer of amino acids. Fermentation of amino acids will produce a large amount of waste bacteria. At present, most of the bacteria are prepared into feed additives, which can achieve certain economic benefits, but they are low-end products, and a small number of manufacturers apply the bacteria to fertilizer preparation. The applicant's previous patented technology "...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/12C07K1/14C07K1/34A23K10/16A23K10/37A23K10/26
CPCC07K1/12C07K1/14C07K1/34Y02P60/87
Inventor 刘海涛丁兆堂卢松伏广好李晓永许传娟杜鹏徐亚娟位风宇
Owner 内蒙古阜丰生物科技有限公司
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