Method for Simultaneously Detecting Five Steroid Hormones in Serum

A steroid hormone and serum technology, applied in the field of analysis and detection, can solve the problems of high reagent cost, matrix interference, and low detection limit, and achieve the effects of strong anti-matrix interference, removal of matrix interference, and reduced cost of consumables
CN107064400BActive Publication Date: 2018-09-21GUANGZHOU BIOHOP TECH INC
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGZHOU BIOHOP TECH INC
Publication Date
2018-09-21

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Abstract

The invention relates to a method for simultaneously detecting five kinds of steroid hormones in serum. The five kinds of steroid hormones are respectively testosterone, androstenedione, 11-deoxycortisol, cortisol and cortisone. The method for simultaneously detecting the five kinds of steroid hormones in the serum comprises the following steps: sample pretreatment: adding an acetonitrile solution of an internal standard substance in a serum sample for carrying out protein precipitation, then adding tert-butyl methyl ether for extracting, blow-drying supernatant, adding a combination solution, and taking the supernatant, thus obtaining a to-be-detected sample; enrichment, separation and detection: carrying out enrichment, separation and detection on the to-be-detected sample by adopting a two-dimensional liquid chromatography-tandem quadrupole mass spectrometer. According to the method for simultaneously detecting the five kinds of steroid hormones in the serum, disclosed by the invention, the method is adopted for simultaneously detecting five kinds of compounds of which the concentration ranges are inconsistent in the serum, and the time for simultaneously detecting the five kinds of the compounds is about 8.5 to 12.0 minutes; the method for simultaneously detecting the five kinds of steroid hormones in the serum is low in cost, high in flux, high in precision degree and strong in specificity.
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Description

technical field

[0001] The invention relates to the technical field of analysis and detection, in particular to a method for simultaneously detecting five steroid hormones in serum. Background technique

[0002] Testosterone is a steroid hormone that is secreted by the testes in men or the ovaries in women, and a small amount by the adrenal glands, but in postmenopausal women, the secretion of testosterone from the ovaries is significantly reduced. The molecular weight of testosterone is 288.42g / moL, and its structural formula is as follows. Most testosterone is bound to sex hormone-binding globulin (SHBG), a small amount is bound to albumin, and a small portion exists in free form. All non-SHBG-bound testosterone is considered bioavailable. During childhood, excess testosterone induces precocious puberty in boys and virilization in girls. In adult women, excess testosterone can cause varying degrees of virilization, including hirsutism, acne, oligomenorrhea, or infertilit...

Claims

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