Method of adsorbing bivalent heavy metal ions in wastewater by means of morchella mycelia
A technology of heavy metal ions and Morchella, applied in the direction of adsorption of water/sewage treatment, chemical instruments and methods, water pollutants, etc., to achieve the effect of easy-to-obtain raw materials, large adsorption capacity, and fast adsorption speed
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Embodiment 1
[0035] 1) the cultivation of hickory tripe mycelium;
[0036] Under aseptic conditions, the morels were inserted into the slant culture medium, cultured at 25°C for 7 days, and the morels in the test tubes of the slant culture medium with strong mycelium growth, dense and pollution-free were selected as strains. Under sterile conditions, insert 0.5cm into each conical flask of liquid medium 2 Put 4 pieces of morel mycelium test tube strains into a constant temperature shaking incubator at 25°C and a speed of 100r min -1 After 7 days of shaking culture under the same conditions, the morels in the Erlenmeyer flasks with clear medium, no turbidity, good growth of mycelium balls and no contamination were selected for the preparation of morel mycelium biomass adsorbent.
[0037] 2) preparing morel mycelia biomass adsorbent;
[0038] Place the selected morel mycelium in the Erlenmeyer flask on filter paper, put it in an oven at 70°C for 4 hours, take it out, and grind it into powd...
Embodiment 2
[0040] Accurately weigh 20 mg of morel mycelia prepared in [Example 1] and other 3 kinds of thalline (saccharomyces, aspergillus oryzae and aspergillus niger), and add the same pH = 6.0 to a 25mL stoppered colorimetric tube. Concentration of a single metal ion solution, where Cu 2+ The concentration is 10mg·g -1 ;Cd 2+ and Mn 2+ The concentration is 5mg·g -1 ; Zn 2+ The concentration is 1mg·g -1 ; At 25°C, ultrasonically oscillate for 15 minutes, stand still for 15 minutes, filter, take the filtrate, measure the absorbance value with an atomic absorption spectrophotometer, calculate the concentration of each metal ion in the raffinate, and then calculate the effects of the four bacteria on copper, cadmium, The adsorption capacity of manganese and zinc, the results are shown in Table 1.
[0041] The adsorption capacity of bacteria to divalent heavy metal ions is calculated according to the following formula (the same below):
[0042]
[0043] In the above formula, q i...
Embodiment 3
[0048]The hickory tripe mycelium powder that [embodiment 1] prepares is respectively soaked in concentration all is 0.4mol L -1 Hydrochloric acid, nitric acid and sodium hydroxide solution, ultrasonic vibration in an ultrasonic vibrator for 20 minutes, washed with water until neutral, filtered, placed in a constant temperature drying oven, dried at 70 ° C for 4 hours, after drying, the processed Morchella mycelium biomass adsorbent, sealed and stored for later use.
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