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A kind of enterococcus faecalis microcapsule and preparation method thereof

A technology for Enterococcus faecalis and microcapsules is applied in the field of Enterococcus faecalis microcapsules and preparation thereof, and can solve the problems affecting the industrialization and application of active products of coated lactic acid bacteria, intolerance of coating materials, toxic cross-linking agents, and the like, To achieve the effect of easy expansion of production, good protection effect, and guaranteed activity

Active Publication Date: 2020-02-11
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, most of the existing microcapsule coating technologies and products have unfavorable factors such as surface porosity, coating material intolerance to gastric acid, toxic cross-linking agent, and technical process not suitable for large-scale production and application, which seriously affect the quality of the coated lactic acid bacteria. The activity and the industrialization and application of its products

Method used

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  • A kind of enterococcus faecalis microcapsule and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 Enterococcus faecalis microcapsules and preparation method thereof

[0034] The bacterial strain used in this example is Enterococcus faecalis CGMCC1.2024.

[0035] (1) 10.0g peptone, 5.0g beef powder, 4.0g yeast powder, 20.0g glucose, 1.0mL Tween 80, CH 3 COONa·3H 2 O 5.0g, K 2 HPO 4 ·7H 2 O 2.0g, triammonium citrate 2.0g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 4H 2 O 0.05g, agar 15.0g, add distilled water to dissolve, set the volume to 1000mL, pH6.2±0.2, sterilize by steam at 115℃ for 20min, dump the plate to make a solid plate medium for activated bacteria.

[0036] Peptone 10.0g, beef powder 10.0g, yeast powder 5.0g, glucose 20.0g, magnesium sulfate 0.1g, sodium acetate 5.0g, ammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.05g, Tween 80 1.0 mL, add distilled water to dissolve, dilute to 1000mL, pH7.3±0.2, dispense into Erlenmeyer flasks, steam sterilize at 115°C for 20min, and make cell seed liquid and expansion culture liq...

Embodiment 2

[0040] Morphology and property analysis of embodiment 2 Enterococcus faecalis microcapsules

[0041] Harvest the Enterococcus faecalis microcapsules prepared in Example 1, and observe the morphology, particle size, embedding efficiency of the microcapsules and evaluate the protective effect on the target bacteria in the gastrointestinal fluid, and at the same time and separate sodium alginate coating The coating effect of microcapsules was compared and analyzed.

[0042] The microcapsule prepared in embodiment 1 is compared with the microcapsule prepared by independent sodium alginate:

[0043] 1. The microcapsules prepared by the present invention have better sphericity, and smooth and compact surface structure (the granular structure visible on the surface is the bacterium adhered to the surface), effectively filled with separate sodium alginate microcapsules to form porous structure ( figure 1 ).

[0044] 2. The microcapsules prepared by the present invention have a coat...

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Abstract

The invention provides an Enterococcus faecalis microcapsule and a preparation method thereof. The preparation method is to use sodium alginate and konjac gum as a composite wall material, dissolve and sterilize it, mix it with the target bacteria suspension and stir evenly, and then add it to large Soybean oil, stir on a magnetic stirrer until fully emulsified, then quickly add CaCl along the wall 2 solution, stirred at a low speed until the water and oil phases separated, and finally centrifuged at a low speed to collect the microcapsules, and washed with CaCl 2 After rinsing with the solution, the microcapsules were collected under the same conditions and placed at 4°C overnight. Finally, enterococcus faecalis microcapsules were obtained by filtering excess water with sterile gauze. The microcapsules prepared by the present invention have better physical form and protective effect on target bacteria, and the preparation method used is simple to operate and is convenient for expanding production; the wall material used is natural and safe, and has strong resistance to gastric acid; Toxic cross-linking agent ensures the activity of coated lactic acid bacteria.

Description

technical field [0001] The invention belongs to the technical field of microcapsules, and in particular relates to a microcapsule of Enterococcus faecalis and a preparation method thereof. Background technique [0002] Humans have a long history of using lactic acid bacteria. After years of research and development, most lactic acid bacteria and their metabolic activities have played important functions in food, industry, agriculture, medicine and scientific research. Especially in medicine, a large number of existing studies have shown that lactic acid bacteria play an indispensable role in lowering blood pressure, treating diabetes, suppressing obesity, and regulating emotions by improving the intestinal flora of animals. In addition, it can produce a variety of metabolites to inhibit pathogenic bacteria and maintain the balance of animal digestive tract flora; it has nutrient metabolism and promotes the growth of livestock and poultry; enhances the intestinal immune funct...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A61K47/36A61K35/744C12N11/10C12N11/08C12N11/04A23L33/135A23L33/125A23P10/30A23K10/18A23K20/163C12R1/46
CPCA23K10/18A23K20/163A23L33/125A23L33/135A23P10/30A61K9/5036A61K35/744C12N11/04C12N11/08C12N11/10A23V2002/00A23V2400/225A23V2200/224A23V2200/30A23V2250/5026A23V2250/5066
Inventor 王建华杨新建滕达王秀敏毛若雨郝娅
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES