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Application of carbonate-mineralization microbe in decreasing content of effective-state cadmium in soil and method

A technology of mineralizing bacteria and carbonate, applied in the field of resources and environment, can solve the problems of complex remediation and treatment operations, high remediation costs, soil cadmium pollution, etc., and achieves the effects of low cost, strong tolerance and simple operation.

Inactive Publication Date: 2018-01-05
BEIJING GEOENVIRON ENG & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The first object of the present invention is to provide a kind of application of carbonate mineralizing bacteria in reducing the available content of cadmium in soil, and the second object of the present invention is to provide a kind of utilization of carbonate mineralizing bacteria to reduce the content of cadmium in soil. The method of cadmium effective state content is used to alleviate the serious soil cadmium pollution in the existing technology, and the restoration and treatment operation is complicated, the restoration cost is high, and there is secondary pollution. Research on the problem of remediation of heavy metal pollution by microbial-induced carbonate mineralization technology Rarely reported technical issues

Method used

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  • Application of carbonate-mineralization microbe in decreasing content of effective-state cadmium in soil and method
  • Application of carbonate-mineralization microbe in decreasing content of effective-state cadmium in soil and method

Examples

Experimental program
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Effect test

Embodiment 1

[0058] Example 1 Isolation and identification of strains

[0059] The heavy metal-contaminated soil in the mining area was collected, and pure single bacteria were obtained by using a specific selection medium for repeated streaking and isolation.

[0060] Among them, selection medium (g / L): yeast extract 10, NH 4 Cl 20, anhydrous sodium acetate 8.2, urea 40.4, deionized water. Urea and ammonium chloride were sterilized by filtration with a 0.22-micron filter membrane, and the remaining components were sterilized at 121°C for 20 minutes under high pressure and high temperature. Identification medium (g / L): peptone 10, beef extract 3, urea 40.4, sodium chloride 5, agar powder 15, 1.6% bromocresol purple 2, deionized water. The urea and bromocresol purple solutions were sterilized by filtration, and the remaining components were sterilized by autoclaving at 121°C for 20 minutes. Finally, 2mol / L NaOH solution was used to adjust the pH of the medium to 5.75. LB medium (g / L): p...

Embodiment 2

[0064] Example 2 Soil remediation

[0065] Inoculate the sterilized 100mL LB medium with the strain provided in Example 1, shake and culture at 25°C for 24 hours, measure the OD value of the bacterial solution with an ultraviolet spectrometer, and ensure that the OD value of the bacterial solution is consistent with the soil before mixing. Dilute the bacterial solution to OD value = 1.5 and set aside.

[0066] Take 500g of soil with a cadmium content of 10mg / kg, air-dry it naturally, grind it through a 20-mesh sieve, and mix it with the cultured bacterial solution and urea. The mass ratio of urea, bacterial solution and soil is 0.05:1:5, that is, take 100g of solution, 500g of soil to be repaired, added 5g of urea, stirred evenly and cultured at 25°C, and the soil moisture content was maintained at 35% during the test. Set up a blank group and a control group, where the blank group refers to the campus soil without adding cadmium solution, maintaining a moisture content of 35...

Embodiment 3

[0067] The mensuration of embodiment 3 cadmium content

[0068] After 7 days and 14 days of restoration culture, the TCLP method was used to measure the effective content of Cd in the soil before and after treatment, respectively. In this embodiment, the buffer solution was selected to detect the test samples. Among them, the configuration method of buffer solution is: 5.7mL glacial acetic acid is dissolved in distilled water, use 1L volumetric flask to constant volume, 1M HNO 3 and 1M NaOH were used to adjust the pH of the buffer so that the pH was 2.88±0.05. Mix the buffer solution with the repaired soil, the mass ratio of the buffer solution to the soil is 20:1, shake and react for 18 hours under the condition of 30±2r / min, centrifugal filter, and use ICP to measure the cadmium concentration in the supernatant. The result is as figure 1 with figure 2 Shown, wherein, monomeric urease activity (SUA) refers to the urease activity of bacterial strain under the unit OD value...

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Abstract

The invention provides application of a carbonate-mineralization microbe in decreasing the content of effective-state cadmium in soil and a method, and relates to the technical fields of resources andenvironment. According to the carbonate-mineralization microbe, it is found that the content of the effective-state cadmium in the soil can be effectively decreased through experiments, and meanwhilethe carbonate-mineralization microbe is high in endurance capacity and can adapt to a bad living environment. According to the method for decreasing the content of the effective-state cadmium in thesoil through the carbonate-mineralization microbe, carbonate-mineralization microbe liquid, the soil to be remedied and nutrient substances are mixed in proportion and then cultivated, a remedying effect can be achieved, operation is easy, and the cost is low. Heavy metal contamination is remedied by adopting the microbe, no secondary contamination is generated, and the carbonate-mineralization microbe has a wide application prospect in the aspect of heavy metal contamination remediation of the soil.

Description

technical field [0001] The invention relates to the technical field of resources and environment, in particular to the application and method of carbonate mineralizing bacteria in reducing the available cadmium content in soil. Background technique [0002] Since the discovery of cadmium in the early 20th century, Cd has been widely used in electroplating industry, chemical industry, electronics industry, nuclear industry and other fields, and the demand is also increasing. A considerable amount of Cd is discharged into the environment through waste gas, waste water, and waste residue, causing Serious environmental pollution. Metal cadmium is a typical heavy metal element and one of the five most dangerous factors in the environment. Its toxicity is more than 5 times that of lead. Most of it comes from smelting, chemical industry, chrome plating industry, waste manufacturing, garbage incineration and tailings piles, etc. . Cd 2+ It can enter the human body through the foo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
Inventor 赵越魏丽苗竹李淑彩朱湖地宋登慧李静文刘泽军吕正勇
Owner BEIJING GEOENVIRON ENG & TECH
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