Method for reducing diseases and accelerating seedling emergence of tobacco seeds

Abstract

The invention discloses a method for reducing diseases and accelerating seedling emergence of tobacco seeds. The method comprises the following four steps: carrying out seed disinfection, acceleratinggermination of seeds by means of a thin layer of water film, carrying out quantitatively hill-seeding, and enabling the seeds with fixed roots to uninterruptedly germinate under the conditions of heat preservation and moisture preservation; pathogenic bacteria and viruses, which are carried by the seeds, can be killed by means of the seed disinfection; the key technologies such as quantitativelyhill-seeding, heat preservation and moisture preservation can be realized by accelerating germination and amplifying seed particles, so that aims of reducing the diseases and accelerating the seedlingemergence are achieved; according to the types of the viruses and pathogenic bacteria which are carried by the tobacco seeds, and the like, a method combining physics and chemistry is used for killing the viruses and the pathogenic bacteria based on the principle of not obviously affecting seed germination, so that toxin sources are reduced. Compared with direct sowing and seedling raising, the method provided by the invention has effects of detoxification and sterilization at the same time, is especially obvious in detoxification effect, has a relative control effect reaching up to 90% or above, and saves the seeds; furthermore, the method reduces the work load of thinning, and shortens 7-14 days of heat preservation and moisture preservation time, thus being applicable to breeding seedlings in seed reproduction and comparison experiment processes of tobacco resource / line / strain materials.

Description

technical field [0001] The invention belongs to the technical field of tobacco cultivation, and in particular relates to a method for reducing diseases and accelerating the emergence of tobacco seeds. Background technique [0002] Tobacco intensive seedling cultivation has higher and higher requirements on the sowing quality of tobacco seeds. Germination-coated pelletized tobacco seeds solve the problems of waste of seeds and time-consuming thinning of seedlings in traditional seedling cultivation methods. Compared with conventional coated species, the emergence time is greatly shortened, the emergence rate, quality and uniformity of tobacco seedlings are significantly improved, and the disease resistance is significantly enhanced (Ma Wenguang et al. Application of seed priming and germination-promoting coating pilling technology in tobacco leaf production in my country. Zhejiang Agricultural Science. 2011(1): 211-215.). However, this large-scale germination method is not sui...

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Problems solved by technology

Existing studies have shown that the average virus-carrying rate of seeds harvested from TMV-infected plants exceeds 50% (Yang Song et al. Comparison of seed-carrying rates after tobacco mosaic virus inoculation at different periods. Zhejiang Agricultural Science, 2005(5 ): 409-410.), the carrier rate of Alternaria Alternaria in tobacco seeds can reach 18%, and that of wildfire bacteria can reach 8-16% (Hua Zhifu et al. Tobacco seed carrier analysis and seed treatment. China Tobacco, 1994(4): 40-42 .), infected seeds during seedling cultivation can cause seedbed viral diseases (Li Fan et al. The main transmission route of common mosaic disease in flue-cured tobacco floating seedling cultivation. Tobacco Science and Technology, 2006(10): 53 -55.), tobacco seeds Tobacco virus-infected seeds and their detoxification treatment. Fujian Tobacco, 1994(2):27 -29.), sterilization (Hu Chongyi et al. Research on seed disinfection technology before aseptic seedling cultivation of tobacco. China Tobacco Science, 2007(2): 5-7) often provides a single technology, which only suppresses the occurrence of a certain type of disease, but cannot take into account multiple types (that is, all types of viruses and bacteria transmitted by tobacco seeds)

Conventional germination experimental bed germination, shaker vibration germination (Chen Tingjun. Research on the new technology of tobacco seed germination. Tobacco Science and Technology, 1997 (1): 42-43.) Often the germination of the seeds is stopped until the seeds are white, and they need to be re-dried when sowing , and then adopt the traditional direct sowing method, that is, on-demand sowing like bare seeds, which cannot overcome the problems of large seeding volume and thinning workload

Therefore, it is necessary to find another way to sterilize, germinate, and sow a small amount of tobacco seeds such as these tobacco resources and breeding intermediate materials, otherwise it will seriously affect the development of subsequent experiments

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