Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation containing male chromosomal stem cells for treating female specific tumors and preparation method thereof

A chromosome and stem cell technology, applied in the field of biomedical technology and applications, to improve the viability and cell activity, relieve pain, and obtain the effects of easy access

Inactive Publication Date: 2018-05-15
奥思达干细胞有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention uses stem cells containing male-specific chromosomes to treat, solve and prevent a series of female breast and reproductive system diseases caused by excessive female estrogen levels

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation containing male chromosomal stem cells for treating female specific tumors and preparation method thereof
  • Preparation containing male chromosomal stem cells for treating female specific tumors and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation containing male chromosomal stem cells for treating female-specific tumors, the preparation comprising 1×10 7 Each / mL male embryo-derived bone marrow mesenchymal stem cells, 80ng / mL peripheral blood plasma protein, and normal saline as the balance, were prepared into 1mL preparation.

[0026] The preparation method of the male embryo-derived bone marrow mesenchymal stem cells is:

[0027] (1) Inoculate the qualified male embryonic bone marrow mesenchymal stem cell suspension in a culture bottle containing serum-containing DMEM / F12 medium, and place the culture bottle at a temperature of 37°C, saturated humidity, and 5% CO 2 Start culturing in an incubator with a high concentration, and replace the culture medium every 2 days to remove unattached cells;

[0028] (2) When cultured for 3 days, the cells were collected by centrifugation after digestion with 0.05% trypsin;

[0029] (3) Repeat the above steps for the cells collected in step (2) to continue sub...

Embodiment 2

[0033] A preparation containing male chromosomal stem cells for treating female-specific tumors, the preparation comprising 2×10 7 1 / mL male embryo-derived bone marrow mesenchymal stem cells, 120 ng / mL peripheral blood plasma protein, and normal saline as the balance, were prepared into 1 mL preparation.

[0034] The preparation method of the male embryo-derived bone marrow mesenchymal stem cells is:

[0035] (1) Inoculate the qualified male embryonic bone marrow cell suspension in a culture bottle containing serum DMEM / F12 medium, and place the culture bottle at a temperature of 37°C, saturated humidity, and 5% CO 2 Start culturing in an incubator with a high concentration, and replace the culture medium every 2 days to remove unattached cells;

[0036] (2) When cultured for 3 days, the cells were collected by centrifugation after digestion with 0.05% trypsin;

[0037] (3) Repeat the above steps for the cells collected in step (2) to continue subculture, until the cells rea...

Embodiment 3

[0041]A preparation containing male chromosomal stem cells for treating female-specific tumors, the preparation comprising 3×10 7 Each / mL male embryo-derived bone marrow mesenchymal stem cells, 160ng / mL peripheral blood plasma protein, and normal saline as the balance, were prepared into 1mL preparation.

[0042] The preparation method of the male embryo-derived bone marrow mesenchymal stem cells is:

[0043] (1) Inoculate the qualified male embryonic bone marrow mesenchymal stem cell suspension in a culture bottle containing serum-containing DMEM / F12 medium, and place the culture bottle at a temperature of 37°C, saturated humidity, and 5% CO 2 Start culturing in an incubator with a high concentration, and replace the culture medium every 2 days to remove unattached cells;

[0044] (2) When cultured for 3 days, the cells were collected by centrifugation after digestion with 0.05% trypsin;

[0045] (3) Repeat the above steps for the cells collected in step (2) to continue sub...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation containing male chromosomal stem cells for treating female specific tumors and a preparation method thereof. The preparation comprises embryo-derived mesenchymalstem cells containing male XY chromosome, peripheral blood plasma proteins and normal saline. By utilizing the stem cells containing the male specific chromosome, diseases of series benign and malignant tumors in organs such as the female mammary gland, uterus, ovary and the like due to extremely high female estrogen level can be treated and prevented.

Description

technical field [0001] The invention relates to the field of biomedical technology and application, in particular to a preparation and a preparation method for treating female-specific benign and malignant tumors prepared by culturing stem cells containing male XY chromosomes. Background technique [0002] Female-specific benign and malignant tumors are gender-specific diseases that endanger women's health and have limited therapeutic effects, especially for female-specific tumors of the breast, cervix, uterus, endometrium, and ovary. Afterwards, it will cause loss of organ function and the cure rate is limited. Traditional surgical treatment faces risks such as anesthesia and surgical accidents, postoperative infection, bacteremia, and local planting. Advanced cancer loses the chance of surgery and can only do radiotherapy and chemotherapy. Radiation therapy has great damage to normal tissues and vital organs, not only skin radiation damage, but also bone marrow damage, a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/28A61P35/00C12N5/0775
CPCA61K35/28C12N5/0663
Inventor 周萱
Owner 奥思达干细胞有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com