Preparation containing male chromosomal stem cells for treating female specific tumors and preparation method thereof

A chromosome and stem cell technology, applied in the field of biomedical technology and applications, to improve the viability and cell activity, relieve pain, and obtain the effects of easy access

Inactive Publication Date: 2018-05-15
奥思达干细胞有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention uses stem cells containing male-specific chromosomes to treat, solve and prevent

Method used

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  • Preparation containing male chromosomal stem cells for treating female specific tumors and preparation method thereof
  • Preparation containing male chromosomal stem cells for treating female specific tumors and preparation method thereof

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Effect test

Embodiment 1

[0025] A preparation containing male chromosomal stem cells for treating female-specific tumors, the preparation comprising 1×10 7 Each / mL male embryo-derived bone marrow mesenchymal stem cells, 80ng / mL peripheral blood plasma protein, and normal saline as the balance, were prepared into 1mL preparation.

[0026] The preparation method of the male embryo-derived bone marrow mesenchymal stem cells is:

[0027] (1) Inoculate the qualified male embryonic bone marrow mesenchymal stem cell suspension in a culture bottle containing serum-containing DMEM / F12 medium, and place the culture bottle at a temperature of 37°C, saturated humidity, and 5% CO 2 Start culturing in an incubator with a high concentration, and replace the culture medium every 2 days to remove unattached cells;

[0028] (2) When cultured for 3 days, the cells were collected by centrifugation after digestion with 0.05% trypsin;

[0029] (3) Repeat the above steps for the cells collected in step (2) to continue sub...

Embodiment 2

[0033] A preparation containing male chromosomal stem cells for treating female-specific tumors, the preparation comprising 2×10 7 1 / mL male embryo-derived bone marrow mesenchymal stem cells, 120 ng / mL peripheral blood plasma protein, and normal saline as the balance, were prepared into 1 mL preparation.

[0034] The preparation method of the male embryo-derived bone marrow mesenchymal stem cells is:

[0035] (1) Inoculate the qualified male embryonic bone marrow cell suspension in a culture bottle containing serum DMEM / F12 medium, and place the culture bottle at a temperature of 37°C, saturated humidity, and 5% CO 2 Start culturing in an incubator with a high concentration, and replace the culture medium every 2 days to remove unattached cells;

[0036] (2) When cultured for 3 days, the cells were collected by centrifugation after digestion with 0.05% trypsin;

[0037] (3) Repeat the above steps for the cells collected in step (2) to continue subculture, until the cells rea...

Embodiment 3

[0041]A preparation containing male chromosomal stem cells for treating female-specific tumors, the preparation comprising 3×10 7 Each / mL male embryo-derived bone marrow mesenchymal stem cells, 160ng / mL peripheral blood plasma protein, and normal saline as the balance, were prepared into 1mL preparation.

[0042] The preparation method of the male embryo-derived bone marrow mesenchymal stem cells is:

[0043] (1) Inoculate the qualified male embryonic bone marrow mesenchymal stem cell suspension in a culture bottle containing serum-containing DMEM / F12 medium, and place the culture bottle at a temperature of 37°C, saturated humidity, and 5% CO 2 Start culturing in an incubator with a high concentration, and replace the culture medium every 2 days to remove unattached cells;

[0044] (2) When cultured for 3 days, the cells were collected by centrifugation after digestion with 0.05% trypsin;

[0045] (3) Repeat the above steps for the cells collected in step (2) to continue sub...

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Abstract

The invention discloses a preparation containing male chromosomal stem cells for treating female specific tumors and a preparation method thereof. The preparation comprises embryo-derived mesenchymalstem cells containing male XY chromosome, peripheral blood plasma proteins and normal saline. By utilizing the stem cells containing the male specific chromosome, diseases of series benign and malignant tumors in organs such as the female mammary gland, uterus, ovary and the like due to extremely high female estrogen level can be treated and prevented.

Description

technical field [0001] The invention relates to the field of biomedical technology and application, in particular to a preparation and a preparation method for treating female-specific benign and malignant tumors prepared by culturing stem cells containing male XY chromosomes. Background technique [0002] Female-specific benign and malignant tumors are gender-specific diseases that endanger women's health and have limited therapeutic effects, especially for female-specific tumors of the breast, cervix, uterus, endometrium, and ovary. Afterwards, it will cause loss of organ function and the cure rate is limited. Traditional surgical treatment faces risks such as anesthesia and surgical accidents, postoperative infection, bacteremia, and local planting. Advanced cancer loses the chance of surgery and can only do radiotherapy and chemotherapy. Radiation therapy has great damage to normal tissues and vital organs, not only skin radiation damage, but also bone marrow damage, a...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P35/00C12N5/0775
CPCA61K35/28C12N5/0663
Inventor 周萱
Owner 奥思达干细胞有限公司
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