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Plant proteinase extraction method and obtained plant proteinase

A plant protease and extraction method technology, applied in the direction of hydrolytic enzymes, etc., can solve the problems of low purity of papain, low enzyme activity and purity, and low extraction specificity, and achieve good enzyme activity and purity, and purification effect Good, low loss effect

Inactive Publication Date: 2018-06-19
XIAN HERB REFINE BIOLOGIC PRODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this method is not strong in extraction specificity, the papain purity that makes is not high, and impurity content is higher
[0008] It can be seen that the existing plant protease extraction method has the defects of complex process, large environmental pollution, low enzyme activity and purity

Method used

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  • Plant proteinase extraction method and obtained plant proteinase
  • Plant proteinase extraction method and obtained plant proteinase
  • Plant proteinase extraction method and obtained plant proteinase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A kind of extraction method of papain, described method is made up of the following steps:

[0044] Mix papaya pulp and process water at a material-to-liquid ratio of 1:10, add enzyme protection agents sodium benzoate and EDTA-2Na, and the mass fractions of the sodium benzoate and the EDTA-2Na in the mixed solution are 0.1% to 0.1%. 0.2%, stirred and reacted for 1.5h to obtain the extract;

[0045] The obtained extract is filtered through a plate and frame filter press and a ceramic membrane successively, the filter cloth aperture of the plate and frame filter press is 150 mesh, and the aperture of the ceramic membrane is 50nm;

[0046] Using an ultrafiltration membrane with a molecular weight cut-off of 50KDa to carry out the first ultrafiltration treatment on the filtrate, collecting the filtrate, and using an ultrafiltration membrane with a molecular weight cut-off of 10KDa to carry out the second ultrafiltration treatment on the filtrate to collect the retentate;

...

Embodiment 2

[0049] A method for extracting mogrosin protease, the method is composed of the following steps:

[0050] Wash and crush fresh Luo Han Guo, centrifuge to remove slag to obtain Luo Han fruit juice, mix Luo Han fruit juice with process water at a material-to-liquid ratio of 1:10, and stir for 1.5 hours to obtain the extract;

[0051] The obtained extract is filtered through a plate and frame filter press and a ceramic membrane successively, the filter cloth aperture of the plate and frame filter press is 150 mesh, and the aperture of the ceramic membrane is 200nm;

[0052] Using an ultrafiltration membrane with a molecular weight cut-off of 50KDa to carry out the first ultrafiltration treatment on the filtrate, collecting the filtrate, and using an ultrafiltration membrane with a molecular weight cut-off of 10KDa to carry out the second ultrafiltration treatment on the filtrate to collect the retentate;

[0053] The retentate is concentrated to obtain a concentrated solution, an...

Embodiment 3

[0055] A kind of extraction method of actinidia protease, described method is made up of the following steps:

[0056] Mix crushed kiwi fruit with pH = 4.5, 24% NaCl aqueous solution with a mass fraction of 1:5, stir for 1.5 hours, and separate solid and liquid after low-temperature precipitation for 6 hours. The buffer is redissolved to obtain the extract;

[0057] The obtained extract is filtered through a plate and frame filter press and a ceramic membrane successively, the filter cloth aperture of the plate and frame filter press is 100 mesh, and the aperture of the ceramic membrane is 100nm;

[0058] Using an ultrafiltration membrane with a molecular weight cut-off of 50KDa to carry out the first ultrafiltration treatment on the filtrate, collecting the filtrate, and using an ultrafiltration membrane with a molecular weight cut-off of 10KDa to carry out the second ultrafiltration treatment on the filtrate to collect the retentate;

[0059] The retentate is concentrated t...

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Abstract

The invention relates to a plant proteinase extraction method and obtained plant proteinase. The method comprises the following steps: mixing slurry or a crushed material of plants with water in a solid-liquid ratio of 1:(5-20), then carrying out water extraction, and collecting an extracting solution; filtering the obtained extracting solution by virtue of a plate-and-frame filter press and a ceramic membrane sequentially, wherein the pore size of filter cloth of the plate-and-frame filter press is 100-150 meshes, and the pore size of the ceramic membrane is 50-200nm; carrying out primary ultrafiltration treatment on a filtrate by using an ultrafiltration membrane with the molecular weight cutoff of 30-100KDa, collecting the filtrate, carrying out secondary ultrafiltration treatment on the filtrate by using an ultrafiltration membrane with the molecular weight cutoff of 5-15KDa, and collecting trapped fluid; and concentrating the trapped fluid to obtain a concentrated solution, and carrying out freeze drying on the concentrated solution, so that the plant proteinase is obtained. The method provided by the invention has the advantages of simple technology, no pollution to the environment, the feasibility of large-scale industrial production and high proteinase activity and purity. The plant proteinase has the advantages of good activity, high purity and no solvent residue and meets usage requirements of the food, daily chemical and medicine industries.

Description

technical field [0001] The invention relates to the field of protease extraction, in particular to a plant protease extraction method and the prepared plant protease. Background technique [0002] Plant proteases have efficient and specific decomposition properties for proteins, and can be used for protein hydrolysis, meat tenderization, beer clarification, seafood processing, preparation of detergents and cosmetics. Certain plant proteases such as papain have pharmacological effects and can be used for Treats inflammation and indigestion. Therefore, plant proteases are widely used in the fields of food, daily chemicals and medicine. Plant protease extraction process, especially the improvement of industrial extraction methods, is of great significance. [0003] At present, there are many extraction and purification methods of plant protease, and there are mainly three extraction methods of plant protease that can be industrialized: kaolin adsorption method, tannin precipi...

Claims

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Application Information

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IPC IPC(8): C12N9/50
CPCC12N9/63
Inventor 杨立琛钱英徐小琴
Owner XIAN HERB REFINE BIOLOGIC PRODS
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