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Electrochemical sensor for sensitively detecting dopamine (DA) and uric acid (UA) simultaneously and preparation method

An electrochemical and dopamine technology is applied in the field of preparation of microwave exfoliated porous carbon modified glassy carbon electrodes, which can solve the problems of inability to detect dopamine and uric acid, difficulty in realizing trace detection, and high detection limit, and achieve a wide detection linear range and good conductivity. the effect of low detection limit

Inactive Publication Date: 2018-07-13
HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] Problems and defects of the prior art: the above-mentioned method cannot selectively detect dopamine and uric acid when high-concentration ascorbic acid exists
Although the invention can realize the simultaneous detection of ascorbic acid, dopamine, uric acid, tryptophan and nitrite, but the detection limit is high, and it is difficult to realize trace detection

Method used

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  • Electrochemical sensor for sensitively detecting dopamine (DA) and uric acid (UA) simultaneously and preparation method
  • Electrochemical sensor for sensitively detecting dopamine (DA) and uric acid (UA) simultaneously and preparation method
  • Electrochemical sensor for sensitively detecting dopamine (DA) and uric acid (UA) simultaneously and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A preparation method of a modified glassy carbon electrode, comprising:

[0037] Preparation of porous carbon exfoliated by microwave

[0038] First, synthesize graphene oxide by the Hummers method, and then use a microwave oven to heat the graphene oxide for 1 minute under the condition of 100W to obtain exfoliated graphene oxide carbon. Add 400 mg of microwave-exfoliated graphite oxide to 20 mL of 7 mol / L potassium hydroxide solution. The microwave-exfoliated graphite oxide / potassium hydroxide mixed solution was stirred for 24 hours, then filtered and dried, then activated at a high temperature of 800°C for 1 hour under an argon atmosphere, and then the sample was washed with deionized water and dried at 65°C. Finally, the pyrolyzed sample was heat-treated at 800°C for 2 hours in a vacuum atmosphere to obtain the prepared microwave-exfoliated porous graphitic carbon. Microwave exfoliation of porous carbon is a kind of sheet-like structure ( image 3 a and b), with ...

Embodiment 2

[0042] Optimization of electrode dispensing volume

[0043] Repeat Example 1, use a pipette gun to pipette 2, 3, 4, 5, 6, 7, 8, 9 μL of the electrode modification solution obtained in Example 1, drop-coat it on the surface of the polished glassy carbon electrode, and apply the obtained modified electrode For the detection of 10μmol / L dopamine and uric acid. The oxidation peak currents of dopamine and uric acid both increased first and then decreased with the increase of the dripping amount, and the peak current reached the maximum when the dripping amount was 5 μL.

Embodiment 3

[0044] The simultaneous selective detection of embodiment 3 dopamine and uric acid:

[0045] One end of the glassy carbon electrode, reference electrode, and counter electrode prepared by microwave exfoliation of porous carbon materials prepared in Example 2 were immersed in 0.1 mol / L of uric acid containing 10 μmol / L, 10 μmol / L dopamine and 1000 μmol / L ascorbic acid respectively. In phosphate buffer solution (pH=7.0), the other ends of the three electrodes were connected to the electrochemical workstation. Cyclic voltammetry was selected on the electrochemical workstation, the scan rate was set to 100mV / s, and the scan range was set to -0.2V-0.8V.

[0046] Run the cyclic voltammetry technique on the electrochemical workstation, and record the current-voltage curve by the electrochemical workstation, such as Figure 4 As shown, the cyclic voltammetry curves of dopamine and uric acid are obtained, from Figure 4 It can be seen that compared with the bare glassy carbon electro...

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Abstract

The invention aims at providing an electrochemical sensor for sensitively and selectively detecting dopamine (DA) and uric acid (UA) simultaneously and a preparation method. The sensor structurally comprises an electrochemical working station, an electrolytic cell and electrodes, wherein the electrodes include a platinum-wire counter electrode, a silver / silver chloride reference electrode and a working electrode; the working electrode means a glass-carbon electrode modified by microwave-peeled porous carbon. The electrochemical sensor provided by the invention has excellent properties such ashigh sensitivity, good selectivity, wide linear range and low detection limit in detecting the DA and the UA since the microwave-peeled porous carbon for modifying the electrode has excellent properties such as large specific surface area, porous structure, excellent electrocatalytic activity property and good conductivity. The electrochemical sensor can be used for qualitatively and quantitatively detecting the contents of the DA and the UA in actual biological samples.

Description

technical field [0001] The invention relates to the field of electrochemical sensors for trace biomolecules, in particular to a method for preparing a microwave-exfoliated porous carbon-modified glassy carbon electrode for trace analysis of dopamine and uric acid. Background technique [0002] Dopamine, uric acid, and ascorbic acid (AA) usually co-exist in organisms and play an important role in the physiological process of organisms. Some new metabolic abnormalities of organisms are related to the abnormal content of dopamine, uric acid, and ascorbic acid in organisms related. For example, some neurodegenerative diseases in the human body, including schizophrenia, Alzheimer's disease and Parkinson's disease syndrome, are all related to abnormal levels of dopamine in the human body. Excess uric acid can lead to gout and hyperuricemia. Diseases such as scurvy, immunodeficiency, and inefficient free radical scavenging activity have been associated with abnormal levels of asc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/36
Inventor 袁群惠吴淑程干为侯易
Owner HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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