Check patentability & draft patents in minutes with Patsnap Eureka AI!

A preparation method of secreted platelet-rich gel for chronic skin injury treatment

A skin-damaged, platelet-rich technology, applied in biochemical equipment and methods, skin diseases, medical raw materials derived from mammals, etc., can solve problems such as volatile loss, immune rejection, and unsatisfactory effects, and achieve simple separation. , The skin has a wide range of sources, and the effect of enhancing the concentration efficiency

Active Publication Date: 2020-12-15
武汉天晴干细胞有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many growth factor-related products and platelet lysate products have been clinically proven to have a certain effect on the treatment of chronic wounds, but most of these products cannot achieve continuous factor secretion and supply. Some are heterologous, and there is a possibility of immune rejection, so the effect is not ideal in clinical practice

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A preparation method of secreted platelet-rich gel for chronic skin injury treatment
  • A preparation method of secreted platelet-rich gel for chronic skin injury treatment
  • A preparation method of secreted platelet-rich gel for chronic skin injury treatment

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0048] Embodiment 1: A preparation method of secreted platelet-rich gel for chronic skin injury treatment is carried out according to the following steps:

[0049] 1. Blood collection and separation of high-concentration PRP

[0050] Peripheral whole blood was collected in sodium citrate blood collection tubes according to the area of ​​chronic skin damage according to the following formula;

[0051] V≥2S~5S; among them, V is the blood collection volume, the unit is mL; S is the wound area, the unit is cm 2 ;

[0052] The collected peripheral blood is separated by the separation method of PRP:

[0053] A. Transport the collected peripheral whole blood to the separation room at 2-8°C. After mixing the peripheral whole blood, take a part and count the platelet concentration for 3 times, and take the average value as the platelet concentration;

[0054] B. Centrifuge the mixed peripheral whole blood to collect platelet-rich plasma. The centrifugation condition is 150-200g for ...

specific Embodiment approach 2

[0074] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the composite collagenase solution contains 0.05-0.1% type I collagenase and 0.03-0.05% IV collagenase by mass percentage. a-MEM solution. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0075]Embodiment 3: This embodiment differs from Embodiment 1 in that: disinfection, rinsing, and cell separation operations are all carried out in a biosafety environment, and all instruments used are sterilized by high-pressure steam. Others are the same as in the first embodiment.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of a secretory platelet-rich gel for treatment of chronic skin injury, and relates to the technical field of activating of platelet-rich gel, which aims at solving the problems of quick degradation and short action time in the existing liquid PRP (platelet-rich plasma) and traditional gel. The preparation method comprises the following steps of collecting a special volume of peripheral blood by blood collecting consumables of sodium citrate anticoagulant according to the injury area of a patient, and the plasma with high platelet content is separated by a special PRP separating technique; collecting the donated fresh and healthy skin tissues to perform primary skin mesenchymal stem cell culture, collecting the fourth and fifth generation type cells, and freeze-drying for standby use. The secretory platelet-rich gel comprises the PRP, skin mesenchymal stem cell, a medical CaCl2 (calcium chloride) solution and platelet-lack plasma containinggentamycin sulfate; after mixing, the PRP and the skin mesenchymal stem cell are spread into a sterile bottle dish, and the gel is prepared by utilizing the inhibiting function of Ca<2+> on the sodiumcitrate anticoagulant.

Description

technical field [0001] The invention relates to a preparation method of secreted platelet-rich gel. Background technique [0002] Chronic skin injury refers to the inability to pass through the normal healing stages due to a variety of systemic and local factors, and cannot repair and restore the integrity of anatomical structure and function in a timely and orderly manner. The reasons include the following four aspects: (a) wound infection or a large amount of necrotic tissue retention; (b) vascular insufficiency and poor local microcirculation in the wound; (c) reduced content and activity of growth factors and out-of-control regulation among various growth factors; (d) cell repair In the case of excessive apoptosis, the structure of cell membrane receptors changes, the response to signals is slow, and there is a response barrier between growth factors and receptors. Clinically, chronic skin injuries mostly refer to wounds caused by various reasons that have received trea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/16A61K35/19A61K35/28A61K9/06A61P17/02C12N5/0775
CPCA61K9/06A61K35/16A61K35/19A61K35/28A61P17/02C12N5/0668C12N2500/25C12N2500/38A61K2300/00
Inventor 刘春香张怡李妍刘艳青
Owner 武汉天晴干细胞有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com