A kind of dressing prepared by using freeze-dried calcium alginate/vaterite calcium carbonate composite microspheres and its preparation method
A technology of composite microspheres and calcium alginate, which is applied in bandages, medical science and other directions, can solve the problems of easy infection of wounds and slow recovery speed, and can promote repair and healing, avoid frequent dressing changes, and prevent bacteria from breeding and infecting wounds. Effect
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Embodiment 1
[0040] A kind of dressing that utilizes freeze-dried calcium alginate / vaterite calcium carbonate composite microspheres to prepare, antibacterial drug in the present embodiment is rifamycin sodium as an example, cell growth factor is mouse basic fibroblast growth factor, The dressing is prepared as follows:
[0041] (1) Preparation of vaterite calcium carbonate suspension by co-precipitation method: adding Na concentration of 50mM in a beaker with a capacity of 100mL 2 CO 3 +8mg / mL casein 20mL, 600rpm magnetic stirring, magnet diameter 40mm, at the same time add 20mL50mM CaCl in the separatory funnel 2 , Open the cock just above the beaker and add evenly. Stir magnetically for 20 minutes. After the stirring is completed, the carbonate ions and calcium ions in the solution react completely at this time to obtain CaCO 3 Suspension, i.e. vaterite calcium carbonate suspension, in which CaCO 3 The concentration is 2.5mg / mL.
[0042] (2) Preparation of rifamycin sodium solution...
Embodiment 2
[0053] (1) Prepare a kind of dressing that utilizes freeze-dried calcium alginate / vaterite calcium carbonate composite microspheres to prepare according to the step in Example 1, wherein for the convenience of observation and detection, when preparing the internal phase, trypan blue (dye ) to replace bacteriostatic drugs or cell growth factors as loaded drugs, observe the prepared freeze-dried composite microspheres, and its morphology under an optical microscope is as follows: figure 2 shown. The light microscope image of the freeze-dried composite microspheres after re-swelling is shown in image 3 shown.
[0054] From figure 2 and image 3 It can be seen from the figure that after lyophilization, the composite microspheres re-swelled to form a hydrogel state, and their spatial structure was more plump and three-dimensional, which did not change much compared with that before lyophilization.
[0055] (2) Referring to the method and steps in (1), trypan blue was used in...
Embodiment 3
[0059] The antibacterial effect of the composite microspheres loaded with drugs was tested as follows:
[0060] (1) Staphylococcus aureus was inoculated in culture medium (LB, 1% peptone, 0.5% yeast extract and 1% NaCl), and shaken at 200 rpm for 24 hours at 37°C. Take a portion of the bacterial suspension and dilute it using the gradient method. The bacterial concentration after counting is 8*10 8 CFU / mL. Draw 100 μL and spread evenly on the LB agar medium in the 90mm Petri dish.
[0061] (2) Taking the composite microsphere A prepared in Example 1, the composite microsphere A and the composite microsphere B mixed in 1:1 as the test object, the composite microsphere Place A and composite microsphere C on a 90mm filter paper sheet, then transfer the filter paper sheet to the center of the medium in step (1), and culture for 24 hours. In order to analyze the sustained antibacterial effect of the experimental group, the above-mentioned filter paper pieces were transferred to...
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