Fondcl1 gene deletion mutant of watermelon Fusarium wilt RNAi component and its construction method

A watermelon wilt pathogen and gene deletion technology, applied in the field of bioengineering, can solve problems such as environmental pollution, lack of long-term antigens in disease-resistant breeding, and ecological destruction
CN108728369BActive Publication Date: 2019-09-10ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
Publication Date
2019-09-10

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Abstract

The invention discloses a fusarium oxysporum f.sp.niveum RNAi component FonDCL1 gene deletion mutant and a construction method thereof. According to the fusarium oxysporum f.sp.niveum RNAi component FonDCL1 gene deletion mutant and the construction method thereof, according to a reported Dicer like 1 protein sequence, homologous alignment is conducted on a Fon genomic sequence to obtain a fusariumoxysporum f.sp.niveum FonDCL1 gene, by adopting a homologous gene substitution principle, by means of a Split-marker strategy, the target gene FonDCL1 is substituted for a hygromycin B (HPH) gene DNAfragment, and by constructing a homologous gene recombinant fragment, through genetic transformation of a wild strain Fon1, the FonDCL1 gene deletion mutant is obtained. The FonDCL1 mutant has the advantages that the virulence to watermelon seedlings is enhanced, and the FonDCL1 mutant is more sensitive to abiotic stress factors of monensin sodium salt, hydroxycarbamide, a fluorescent whitening agent CFW, Congo red (CR), hydrogen peroxide and the like, so that a research foundation is provided for functional assignment of small RNA generated in processes of self growth and development and watermelon infection of the Fon1.
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Description

technical field

[0001] The invention relates to a FonDCL1 gene deletion mutant of the RNAi component of Fusarium wilt of watermelon and a construction method thereof, belonging to the technical field of bioengineering. Background technique

[0002] Fusarium wilt of watermelon is a watermelon vascular system disease caused by Fusarium oxysporum f.sp.niveum infection. The pathogen mainly invades through the root wound or the intercellular space at the top of the root hair. After growing in the cells, it enters the vascular bundle, decomposes and destroys the cells, accumulates pectin substances in the ducts, blocks the ducts, affects water transport, and causes plant wilting.

[0003] Fusarium wilt of watermelon can occur from seedlings to adult plants, and the incidence is the most serious in the stage of melon setting and the later stage of melon expansion. Injured seedlings can cause rotten seeds before emergence and become ill after emergence. The cotyledons and true leav...

Claims

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