A kind of bovine lactoferrin peptide-human lysozyme fusion protein, gene and application thereof

A bovine lactoferrin peptide and fusion protein technology, applied in the field of bovine lactoferrin peptide-human lysozyme, can solve the problems of high purification cost and unhelpful product function, achieve broad antibacterial spectrum, strong antibacterial activity, and improve piglet growth performance Effect
CN108794635BActive Publication Date: 2021-03-19ZHEJIANG UNIV OF TECH
9 Cites 1 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG UNIV OF TECH
Publication Date
2021-03-19

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses bovine lactoferricin-human lysozyme fused protein. The bovine lactoferricin-human lysozyme fused protein is characterized by comprising porcine myofibrillar protein antioxidative peptide, bovine lactoferricin, hen egg white protein antioxidative peptide, porcine muscle myosin antioxidative peptide, flexible peptide and human lysozyme which are connected in series. Through anionic antioxidative peptide derived from fusion expression animals, the positive charge of antimicrobial peptide is neutralized, and inhibition to host bacteria by antimicrobial peptide is reduced; antioxidative peptide is beneficial to the improvement of storage stability of the antimicrobial peptide, the antibacterial activity to escherichia coli (ATCC 25922) by the fused protein is only reduced by 7.7 percent after the fused protein is laid for 20d at 4 DEG C, and the loss of activity to the escherichia coli (ATCC 25922) by the fused protein is only reduced by 15.4 after the fused proteinis laid for 30d.
Need to check novelty before this filing date? Find Prior Art

Description

[0001] (1) Technical field

[0002] The invention relates to bovine lactoferrin peptide-human lysozyme, gene and application thereof.

[0003] (2) Background technology

[0004] The abuse of antibiotics in the field of medical treatment and animal breeding has caused the prevalence of drug-resistant bacteria, posing a serious threat to human health. There is an urgent need to develop new antibacterial drugs or feed additives to replace antibiotics. Antimicrobial peptides and lysozymes are a class of natural peptides and proteins with bactericidal activity, which have been widely used in clinical medicine and food industry as antibiotic substitutes.

[0005] The antibacterial mechanism of antimicrobial peptides is that positively charged antimicrobial peptides bind to negatively charged bacterial cell membranes, causing bacterial death by causing membrane perforation or inhibiting the physiological metabolic process of bacteria. The N-terminus of bovine lactoferrin contains tw...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More