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Chitosan and microorganism compound preparation as well as preparation method and application thereof

A compound preparation, chitosan technology, applied in the direction of drug combination, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problems of water quality deterioration, environmental and human unsafety, etc., to improve water quality, enhance immunity, Improve the effect of animal gut

Active Publication Date: 2018-11-16
INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the problems of deterioration of water quality in the high-density breeding mode of soft-shelled turtles and unsafe drugs for the prevention and treatment of soft-shelled turtle diseases to the environment and human body, the present invention provides a chitosan that can effectively improve the water quality of the breeding water, improve the immunity of soft-shelled turtles and increase their weight. Sugar-microbial compound preparation, safe and environmentally friendly

Method used

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  • Chitosan and microorganism compound preparation as well as preparation method and application thereof
  • Chitosan and microorganism compound preparation as well as preparation method and application thereof
  • Chitosan and microorganism compound preparation as well as preparation method and application thereof

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preparation example Construction

[0034] The present invention also provides a method for preparing the chitosan-microbial composite preparation of the above technical solution, which includes the following steps:

[0035] A. Anaerobic fermentation of Lactobacillus with MRS liquid medium to obtain Lactobacillus fermentation broth;

[0036] B. Mixed fermentation of Bacillus subtilis, Bacillus licheniformis and Bacillus polymyxa with the improved beef extract peptone medium to obtain the compound Bacillus fermentation broth;

[0037] In terms of mass percentage, the improved beef extract peptone medium includes beef extract 0.1-0.5%, peptone 0.3-0.8%, sucrose 0.1-0.6%, sodium chloride 0.05-0.4%, dipotassium hydrogen phosphate 0.01-0.05% , Potassium dihydrogen phosphate 0.02~0.08% and the balance of water, pH 6.8~7.2;

[0038] C. Mix the fermentation broth of Lactobacillus, the fermentation broth of Bacillus complex and chitosan, and adjust the pH to 6.8-7.0 to obtain a chitosan microbial composite preparation;

[0039] T...

Embodiment 1

[0054] The MRS liquid medium was prepared, and Lactobacillus (ATCC393) was inoculated into the MRS liquid medium with an inoculum amount of 10%, and anaerobic fermentation was carried out at 26° C. for 90 hours to obtain a fermentation broth of Lactobacillus containing 550 million bacteria / ml.

[0055] Preparation of improved beef extract peptone medium: beef extract 0.3%, peptone 0.5%, sucrose 0.3%, sodium chloride 0.2%, dipotassium hydrogen phosphate 0.03%, potassium dihydrogen phosphate 0.05%, pH 6.8-7.2 and the balance Inoculate Bacillus subtilis (CICC 20445), Bacillus licheniformis (CICC 23584) and Bacillus polymyxa (ATCC 842) with 10% of the inoculum amount, and mix at 27℃ and 20% dissolved oxygen concentration. After 75 hours of fermentation, a complex bacillus fermentation broth with a bacterial content of 520 million cells / ml is obtained.

[0056] After mixing 12 kg of Lactobacillus fermentation broth, 24 kg of complex Bacillus fermentation broth, 35 kg of bran, 20 kg of z...

Embodiment 2

[0059] The MRS liquid medium was prepared, and Lactobacillus (ATCC393) was inoculated into the MRS liquid medium with an inoculum amount of 10%, and anaerobic fermentation was carried out at 31° C. for 90 h to obtain a lactobacillus fermentation broth containing 500 million bacteria / ml.

[0060] Preparation of modified beef extract peptone medium: beef extract 0.3%, peptone 0.5%, sucrose 0.3%, sodium chloride 0.2%, dipotassium hydrogen phosphate 0.03%, potassium dihydrogen phosphate 0.05% and the balance of water, pH 6.8 ~7.2. Bacillus subtilis (CICC 20445), Bacillus licheniformis (CICC 23584) and Bacillus polymyxa (ATCC 842) were respectively inoculated with 10% inoculum, and mixed fermentation was carried out at 31℃ and 45% for 75h to obtain the bacteria-containing The amount of 260 million / ml compound bacillus fermentation broth.

[0061] After mixing 8 kg of Lactobacillus fermentation broth, 32 kg of compound Bacillus fermentation broth, 30 kg of bran, 20 kg of zeolite powder ...

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Abstract

The invention relates to the field of microorganism preparations, and provides a chitosan and microorganism compound preparation. The chitosan and microorganism compound preparation is prepared from chitosan, lactobacilli and compound bacilli, wherein the compound bacilli comprise bacillus licheniformis, bacillus subtilis and paenibacillus polymyxa, the number of the effective living bacteria is 1.5-2.5 hundred millions / ml, and the pH value is 6.8-7.0. In the chitosan and microorganism compound preparation, by compounding the chitosan, the lactobacilli and the bacilli, the contents of ammonianitrogen, phosphorus and nitrate in a breeding water body can be effectively reduced, so that the water quality of the breeding water body is improved; the chitosan and microorganism compound preparation can also effectively promote soft-shelled turtle growth, and weight gain is improved by at least 15% compared with that of a control group without the chitosan and microorganism compound preparation; meanwhile, the orrho-immunity protein content and the antioxidant capacity of soft-shelled turtles are improved at different degrees, so that the immunocompetence of the soft-shelled turtles is significantly improved.

Description

Technical field [0001] The invention relates to the field of microbial preparations, in particular to a chitosan microbial composite preparation and a preparation method and application thereof. Background technique [0002] Soft-shelled turtle is also known as group fish, foot fish, water fish, king ba, and turtle is rich in nutrients, containing protein, fat, calcium, iron, animal glue, keratin and multiple vitamins. It is a rare tonic. Turtle can also be used as medicine, and its back shell has the effects of nourishing yin and nourishing yang, dispelling masses and calming liver. Turtle is favored by consumers because of its tender meat, delicious taste and high nutritional value. [0003] At present, soft-shelled turtles mainly adopt artificial breeding methods, and artificially cultivated juvenile soft-shelled turtles are mainly cultivated in high density. In the high-density culture mode, the density of young turtles in greenhouses is 25 to 35 per square meter, and the den...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P37/04A61P43/00C02F3/30C02F3/34C02F101/30C02F103/20A61K31/722A61K35/742
CPCA61K31/722A61K35/742A61K35/747A61P37/04A61P43/00C02F3/30C02F3/34C02F2101/30C02F2103/20A61K2300/00
Inventor 张志红王菊华黄江丽何力方春林张国华圣平黄黄贺刚童水明
Owner INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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