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Fe3O4 nanozyme with improved catalytic activity, and preparation method and application thereof

A technology for improving catalytic activity and catalytic activity, which is applied in the field of nanomaterials and biomedical nanotechnology, can solve the problems of low catalytic activity of enzymes and limit practical application value, etc., and achieve high biocompatibility, easy modification and labeling, and storage at room temperature stable effect

Active Publication Date: 2018-12-21
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fe 3 o 4 The application range of nanozymes is getting wider and wider, but the catalytic activity of its enzyme-like enzymes is lower than that of natural enzymes, which limits its practical application value. It is necessary to develop new methods to further optimize and improve its catalytic activity.

Method used

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  • Fe3O4 nanozyme with improved catalytic activity, and preparation method and application thereof
  • Fe3O4 nanozyme with improved catalytic activity, and preparation method and application thereof
  • Fe3O4 nanozyme with improved catalytic activity, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: metal element doping improves Fe 3 o 4 Preparation of nanozymes

[0038] Test materials: 3,3',5,5'-tetramethylbenzidine (3,3',5,5'-Tetramethylbenzidine, TMB), ferric chloride, and ethylene glycol were all ordered from Sigma, and kept away from light at room temperature Store for later use. 30%H 2 o 2 , sodium acetate, calcium chloride, magnesium chloride, copper chloride, zinc chloride, manganese chloride, chromium chloride, nickel chloride, cobalt chloride, tin chloride and lead chloride are all ordered from Shenggong Company and stored at room temperature spare.

[0039] experiment method:

[0040] Metal element-doped nanozymes were prepared by hydrothermal synthesis. First, 0.82 g of anhydrous ferric chloride was completely dissolved in 40 ml of ethylene glycol by magnetic stirring to form a clear solution. Next, slowly add 3.6 g of sodium acetate trihydrate, and stir rapidly to form a uniform suspension. Then add 0.1g of inorganic metal salts: ...

Embodiment 2

[0045] Example 2: Metal element doping enhances Fe 3 o 4 Nanozyme peroxidase activity

[0046] Test materials: 3,3',5,5'-tetramethylbenzidine (3,3',5,5'-Tetramethylbenzidine, TMB) was ordered from Sigma, and stored at room temperature in the dark for future use. 30% H2O2 and sodium acetate were ordered from Shenggong Company and stored at room temperature for future use.

[0047] Method: The enzymatic kinetic parameters of the catalytic substrate H2O2 are measured. The detection method is to take an appropriate amount of nanozyme, dissolve it in 200 μl of 0.1M sodium acetate buffer (pH 4.5), add 4 μl of TMB solution (10mg / ml, dissolve in DMSO), adjust H 2 o 2 The microplate reader detects the light absorption value at 652nm, the time scan is 300s, and the reaction temperature is 37°C. For nanozyme, the concentration gradient of adding H2O2 is 0, 18.625, 37.125, 74.25, 148.5, 297, 594, 1188mM. GraphPadPrism7 software was used to statistically analyze the experimental data...

Embodiment 3

[0049] Example 3: Metal element doping enhances Fe 3 o 4 Nanozyme catalase activity

[0050] Test materials: 30% H2O2 and sodium acetate were ordered from Shenggong Company and stored at room temperature for future use.

[0051] Method: Determination of the enzymatic kinetic parameters of the catalytic substrate H2O2. The detection method is to take an appropriate amount of nanozyme, dissolve it in 5ml of 0.1M sodium acetate buffer solution (pH 7.4), add different amounts of H2O2, and use a dissolved oxygen meter to detect oxygen. generated speed. GraphPad Prism7 software was used for statistical analysis of experimental data, and Michaelis-Menten kinetics was used to analyze catalytic parameters and compare catalytic efficiencies.

[0052] Using a dissolved oxygen meter to detect the effect of metal doping on Fe 3 o 4 effects on catalase-like activity. Such as Figure 6 As shown, with Fe 3 o 4 Compared to Co-Fe 3 o 4 The catalase activity of Mn-Fe was significantly...

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Abstract

The invention provides a Fe3O4 nanozyme with improved catalytic activity, and a preparation method and application thereof. By doping metal elements in Fe3O4 nanozyme, the dismutase activity of peroxidase, catalase and / or superoxide can be improved; the metal element doped Fe3O4 nanozyme can be prepared in large quantities, has low cost, good stability at room temperature, good safety, and good economic value and application prospect.

Description

technical field [0001] The invention belongs to the field of nanomaterials and biomedical nanotechnology, in particular to a Fe with improved catalytic activity 3 o 4 Nanozyme and its preparation method and application. Background technique [0002] Enzyme is a kind of organic molecule with catalytic effect produced by living cells. It has the characteristics of high catalytic efficiency, specific substrate, and mild reaction conditions. However, since the chemical nature of enzymes is proteins, they are prone to structural changes and inactivation in non-physiological environments such as acids, alkalis, and heat. For this reason, scientists have been seeking to prepare artificial mimic enzymes by chemical synthesis for application in non-physiological environments. How to improve the catalytic efficiency of simulated enzymes has always been one of the core issues in this field. [0003] Since 1993, there have been occasional reports of nanomaterial-like enzyme-catalyze...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J23/755B01J23/78B01J23/835B01J23/86B01J23/889
CPCB01J23/755B01J23/78B01J23/835B01J23/862B01J23/8892
Inventor 唐燕阴银燕高利增
Owner YANGZHOU UNIV
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