Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of separation and utilization process of threonine waste mother liquor

A technology of waste mother liquor and threonine, which is applied in the field of threonine mother liquor treatment, can solve the problems of high cost, high cost of medium raw materials, large one-time investment, etc., and achieve load reduction, remarkable ecological and economic benefits, and rapid adaptation Effect

Active Publication Date: 2022-03-29
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no mature complete set of technology applied to production practice at home and abroad.
The main problem is that the one-time investment is too large, or the daily operation cost is too high, most manufacturers cannot bear it, and have to maintain the status quo of excessive emissions for a long time
[0004] The applicant's previous research mainly includes two aspects: 1. The use of waste water as fertilizer preparation is suitable for enterprises with fertilizer production qualifications and capabilities, but for other amino acid production enterprises, the purpose of fertilizer production cannot be achieved; 2. The use of waste water The treatment system purifies it; the heating and concentration process can obtain feed products and remove pollutants at the same time, but the cost is high, time-consuming and laborious, resource recovery is out of touch with wastewater treatment, and there are disadvantages such as easy deterioration of organic matter after high-temperature evaporation and concentration
Studies in the prior art have also shown that amino acid industrial wastewater can be used to produce feed bacterial protein. For example, the Chinese patented technology "Using High Salt Amino Acid Wastewater Fermentation to Produce Feed Debariae hansenii" discloses a method for recycling amino acids A method for fermenting waste water to produce bacterial protein. The method uses waste water to prepare a culture medium. The culture medium is composed of high-salt amino acid sewage: 125g / L-330g / L, carbon source: 30-100g / L, diphosphate Potassium Hydrogen (KH 2 PO 4 ): 5-15g / L, yeast powder: 0-10g / L, magnesium sulfate (MgSO 4 ): 3-9g / L, calcium carbonate (CaCO 3 ): 0.75-2g / L, ferrous sulfate (FeSO 4 ): 1.5-4g / L, and the pH of the medium is 3.5-7.5. This method effectively treats wastewater and prepares bacterial protein; however, this method also has some defects: the cost of medium raw materials is high, and the use of waste mother liquor is limited. and other defects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of separation and utilization process of threonine waste mother liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kind of separation and utilization process of threonine waste mother liquor, it comprises the following steps:

[0033] Threonine waste mother liquor is first filtered through an inorganic ceramic membrane with a molecular weight cut-off of 10,000 Da and a filtration temperature of 32°C to collect bacterial protein, and the filtrate is discharged into an acid-base adjustment tank, and the pH of the liquid is adjusted to 6.5 by adding KOH solution;

[0034]The waste liquid discharged from the acid-base adjustment tank enters the microbial reaction tank, then adds 5wt% rice straw powder, 2wt% wheat bran and 1wt% soybean meal, stirs at 300rpm for 120min, and then inoculates Scenedesmus obliquus according to the inoculum amount of 3%. After cultivating for 12 hours, inoculate the Trichoderma reesei liquid according to the inoculation amount of 6%, after continuing to cultivate for 12 hours, inoculate the Aspergillus niger liquid according to the inoculum amount of 8%, cont...

Embodiment 2

[0044] A kind of separation and utilization process of threonine waste mother liquor, it comprises the following steps:

[0045] Threonine waste mother liquor is first filtered through an inorganic ceramic membrane with a molecular weight cut-off of 10,000 Da and a filtration temperature of 32°C to collect bacterial protein, and the filtrate is discharged into an acid-base adjustment tank, and the pH of the liquid is adjusted to 6.5 by adding KOH solution;

[0046] The waste liquid discharged from the acid-base adjustment tank enters the microbial reaction tank, then adds 5wt% rice straw powder, 2wt% wheat bran and 1wt% soybean meal, stirs at 300rpm for 120min, and then inoculates Scenedesmus obliquus according to the inoculation amount of 5%. After cultivating for 18 hours, inoculate the Trichoderma reesei liquid according to the inoculum of 8%, after continuing to cultivate for 12 hours, inoculate the Aspergillus niger liquid according to the inoculum of 10%, continue to cult...

Embodiment 3

[0056] Synergistic effect test between each microorganism of the present invention:

[0057] 1, test group is embodiment 1; Control group 1 adopts Trichoderma reesei single bacterial strain, all the other are with embodiment 1; Control group 2 adopts Aspergillus niger single bacterial strain, all the other are with embodiment 1; Detected cellulase in culture respectively , glucosidase, acid protease enzyme activity, the specific results are shown in Table 1:

[0058] Table 1

[0059] group Cellulase U / g Glucosidase U / g Alkaline protease U / g test group 3207 413 7516 Control group 1 2639 308 ----- Control group 2 ----- ----- 5745

[0060] Conclusion: Trichoderma reesei and Aspergillus niger selected in the present invention have a better synergistic effect, which greatly improves the enzyme activity of cellulase, glucosidase and acid protease, and contributes to the improvement of sugars and waste proteins in waste liquid. and degrad...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of threonine mother liquor treatment, and discloses a process for separating and using threonine waste mother liquor, which includes the following steps: 1) filtering and adjusting pH, 2) adding agricultural waste, and 3) microbial treatment. The process of the invention can effectively treat the pollutants in the waste mother liquor, and at the same time prepare the feed bacteria and algae protein, save energy and reduce emissions, and simultaneously improve the economic benefits of the enterprise.

Description

technical field [0001] The invention belongs to the technical field of threonine mother liquor treatment, and in particular relates to a process for separating and using threonine waste mother liquor. Background technique [0002] L-threonine is an essential amino acid that constitutes human, animal and plant proteins, and is mainly used in medicine, chemical reagents, food fortifiers, feed additives, etc. According to relevant statistics, the world threonine production was only 4,000 tons in 1993, increased to 25,000 tons in 1999, exceeded 40,000 tons in 2002, and exceeded 200,000 tons in 2015. The rapid development of threonine reflects its importance in the field of amino acid fermentation. [0003] At present, there are three main production methods of threonine: fermentation method, protein hydrolysis method and chemical synthesis method, among which microbial fermentation method has become the mainstream method for threonine production. The production of threonine by...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/12C02F9/14C02F3/34C02F3/32C05F5/00C12R1/89C12R1/885C12R1/685C02F103/36
CPCC12N1/12C12N1/14C02F3/325C02F3/34C02F9/00C05F5/008C02F2305/06C02F2103/36C02F1/66C02F1/44Y02A40/20
Inventor 赵兰坤边建军赵春晓张超垒
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products