Aptamer modified triazine covalent organic framework composite material, preparation method and application

A technology of covalent organic framework and composite material, applied in the field of aptamer-modified triazine covalent organic framework composite material and preparation, can solve the problems of large specific surface area and selectivity limitation, and achieve large specific surface area and stable material properties. , the effect of good crystallization performance

Active Publication Date: 2021-07-27
SUN YAT SEN UNIV
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  • Abstract
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Problems solved by technology

[0003] In analytical chemistry, although it has a large specific surface area, adjustable pore size, and orderly structure, it has a high adsorption capacity or enrichment factor for the target substance, but because its structure itself is an ordered framework structure without functional modification, so In the process of complex sample pretreatment, its selectivity is limited, so it is of great significance to improve the selectivity of materials

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  • Aptamer modified triazine covalent organic framework composite material, preparation method and application
  • Aptamer modified triazine covalent organic framework composite material, preparation method and application
  • Aptamer modified triazine covalent organic framework composite material, preparation method and application

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preparation example Construction

[0033] The preparation method of the aptamer-modified triazine covalent organic framework composite material of the present invention comprises the following steps:

[0034] S1: Preparation of triazine covalent organic framework;

[0035] S2: Disperse the triazine covalent organic framework in a solvent, apply ultrasound and stir, add dropwise an aqueous chloroauric acid solution, centrifuge after stirring, and vacuum dry at room temperature after cleaning to obtain the triazine covalent organic framework of composite gold nanoparticles. It is a dark green product;

[0036] S3: Disperse the triazine covalent organic framework of composite gold nanoparticles in the prepared aptamer solution, shake the reaction in a water bath at a constant temperature, wash with PBS buffer, and obtain the aptamer-modified triazine covalent organic framework composite material .

Embodiment 1

[0039] A preparation method of an aptamer-modified triazine covalent organic framework composite material:

[0040] S1: Take 31.9 mg of 2,4,6-tris(4-aminophenyl)-1,3,5-triazine and 25.6 mg of 2,5-dimethoxyterephthalaldehyde, dissolve them in a solvent O-DCB:DMAC=9:1 (2mL), ultrasonically dispersed for 5min, added 0.2mL of 6mol / L acetic acid, freeze-pump-thaw cycle three times, reacted at 120°C for 3 days, and the product was washed with methanol, di Chloromethane was washed three times respectively, and vacuum-dried at room temperature for 24 hours to obtain a triazine covalent organic framework, and its transmission electron microscope picture is as follows: Figure 1A As shown, the XRD pattern is as figure 2 As shown in a, from Figure 1A It can be seen that the morphology presents a sea cucumber-like structure that is a triazine covalent organic framework, and the protrusions on the surface increase the specific surface area of ​​the material. figure 2 a There is a peak ...

Embodiment 2

[0044] Insulin Enrichment Selectivity Test of Aptamer Modified Triazine Covalent Organic Framework Composite

[0045] The aptamer-modified triazine covalent organic framework composite prepared in Example 1 was applied to the study of the anti-interference effect of the material. In this example, substances with different molecular weights in serum were selected to study the influence of different types of interfering substances on the process of material enrichment of insulin. The selected interfering substances were human immunoglobulin, lysozyme, biotin and the mixture of the first three substances. interferer. During the experiment, the concentration of insulin was 50 ng / mL, and the amounts of several other substances were 50 times that of insulin. Add 10 μL of 20 mg / mL aptamer-modified triazine covalent organic framework composites to 190 μL of insulin and each interferent solution in four times, vortex and shake for 1 h, then centrifuge at 8000 rpm for 6 min, remove the...

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Abstract

The invention relates to an aptamer-modified triazine covalent organic framework composite material and its preparation method and application. The aptamer-modified triazine covalent organic framework composite material of the present invention includes a triazine covalent organic framework, gold nanoparticles and aptamers, and the gold nanoparticles are immobilized on the triazine covalent organic framework On, the aptamer is bound to the surface of the gold nanoparticle. When the sequence of the aptamer is 5′‑SH‑(CH 2 ) 6 ‑(ACAG4TGTG4) 2 -3', the composite material of the present invention can be applied to the enrichment of insulin in biological samples. The aptamer-modified triazine covalent organic framework composite material of the present invention combines the advantages of large specific surface area of ​​covalent organic framework, many adsorption sites and specific recognition of aptamers, has good selectivity, and can Provide an economical, efficient, and highly sensitive bioanalytical method.

Description

technical field [0001] The invention relates to the fields of biomedicine and biological analysis, in particular to an aptamer-modified triazine covalent organic framework composite material, a preparation method and an application. Background technique [0002] Covalent organic frameworks are a new class of materials, which originated from the concept of framework chemistry and are two-dimensional or three-dimensional porous crystal structures composed of organic subatoms through strong covalent bonds. It has the advantages of large specific surface area, adjustable pore size, easy functional modification, and stable chemical properties. These characteristics make it widely used in gas separation and storage, heterogeneous catalysis, drug delivery, chromatographic immobilization separation, etc. Compared with common inorganic microporous materials, covalent organic frameworks can construct materials with different topological structures and microporous properties through d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/22B01J20/30G01N23/20G01N23/2251G01N27/64G01N33/74
CPCB01J20/226G01N23/20075G01N23/2251G01N27/64G01N33/74
Inventor 胡玉玲彭毅李攻科
Owner SUN YAT SEN UNIV
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