Erythrocyte membrane-coated co-supporting gambogic acid and indocyanine green albumin nanoparticle and preparation method and application thereof

A technology of indocyanine green white and red blood cell membrane, applied in the field of bioengineering, can solve the problems of loss of near-infrared absorption ability, structural transformation, optical property change, etc., and achieve the effects of significant curative effect, stable properties and less adverse reactions.

Inactive Publication Date: 2019-03-01
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, ICG is prone to structural changes in physiological media, such as self-aggregation and irreversible

Method used

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  • Erythrocyte membrane-coated co-supporting gambogic acid and indocyanine green albumin nanoparticle and preparation method and application thereof
  • Erythrocyte membrane-coated co-supporting gambogic acid and indocyanine green albumin nanoparticle and preparation method and application thereof
  • Erythrocyte membrane-coated co-supporting gambogic acid and indocyanine green albumin nanoparticle and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: Preparation of albumin gambogic acid indocyanine green nanoparticles

[0030] With bovine serum albumin (BSA) as the substrate, gambogic acid and indocyanine green are adsorbed by hydrophobic force, and the albumin gambogic acid indocyanine green nanoparticles are cross-linked by glutaraldehyde chemical cross-linking agent (GI NPs, the drug-loaded nanocores). The specific method is as follows:

[0031] Dissolve gambogic acid and indocyanine green in 1mL of methanol, and prepare the ratios of different concentrations; weigh bovine serum albumin (BSA) and dissolve it in 10mL of pH 7.4 phosphate buffer, and prepare bovine serum albumin (BSA) with different concentrations. Serum albumin solution. The methanol solution in which gambogic acid (GA) and indocyanine green (ICG) were dissolved was dropped into the stirred bovine serum albumin solution, 20 μL of glutaraldehyde was added for cross-linking, and the stirring was continued in the dark for 24 hours. Fi...

Embodiment 2

[0032] Embodiment 2: the extraction of erythrocyte membrane

[0033] The whole blood of the mouse was put into a centrifuge and centrifuged at 1000rpm and 4°C for 10min to remove the plasma and white blood cell layer; the obtained precipitate, namely red blood cells, was washed with pH 7.4 PBS buffer, and then added with sterilized ultra Pure water, placed in an ice bath for 30-60 minutes to rupture the red blood cells and release hemoglobin, centrifuge at 5000rpm for 10 minutes; finally wash with sterilized ultrapure water for several times to obtain the red blood cell membrane.

Embodiment 3

[0034] Example 3: Preparation of erythrocyte membrane-wrapped albumin gambogic acid indocyanine green nanoparticles

[0035] Take an appropriate amount of red blood cell membrane and disperse it in sterile ultrapure water, add the corresponding proportion of albumin gambogic acid indocyanine green nanoparticles, 100W ultrasound for 3min, and centrifuge at 1000rpm for 5min to obtain gambogic acid and indocyanine green Red blood cell membrane-wrapped albumin gambogic acid indocyanine green nanoparticles (RBCm-GINPs, i.e. red blood cell membrane-wrapped gambogic acid and indocyanine green albumin nanoparticles of the present invention with a content of 22-26% of its mass) ).

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Abstract

The invention discloses an erythrocyte membrane-coated co-supporting gambogic acid and indocyanine green albumin nanoparticle and a preparation method and application thereof. The nanoparticle comprises a drug-loaded nano core and an erythrocyte membrane wrapped outside the drug-loaded nano core. The nanoparticle has high biocompatibility and dispersibility, heat is generated under the irradiationof lasers, tumor cells can be killed, the nanoparticle also has great fluorescence performance, a combination therapy mode of photothermal therapy and chemotherapy under a fluorescence imaging mode can be guided, and through the modification of erythrocytes on the surface of the nanoparticle, long circulation in a living body is achieved, so that the therapeutic effect of the drug-loaded nanoparticle is improved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to red blood cell membrane-wrapped and co-loaded gambogic acid and indocyanine green albumin nanoparticles, a preparation method and application thereof. Background technique [0002] In recent decades, cancer has become one of the most serious diseases that endanger human health. Global population characteristics predict that the incidence of cancer will continue to increase in the next few decades, and by 2025, there will be more than 20 million new cancer cases per year. However, traditional radiotherapy and chemotherapy cannot improve the current situation of high cancer mortality and high treatment costs, and new treatment options are urgently needed to be developed to meet the needs of patients. [0003] As a type of hyperthermia, photothermal therapy uses laser light to irradiate photothermal agents to quickly convert light energy into heat energy, so that...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/46A61K41/00A61K47/64A61K49/00A61P35/00A61K31/352
CPCA61K9/5068A61K31/352A61K41/0052A61K49/0034A61K49/0097A61K47/643A61P35/00A61K2300/00
Inventor 刘源岗王士斌王沛
Owner HUAQIAO UNIVERSITY
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