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A preparation method of natural silk fibroin microfibers regulated by dopamine

A technology of silk fibroin and dopamine, which is applied in the field of preparation of natural silk fibroin microfibers, can solve the problems of lack of reprocessing performance, limited material processing, insufficient dispersibility, etc., and achieves good water dispersibility and surface modifiability, good biocompatibility

Inactive Publication Date: 2021-03-16
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Natural polymer materials such as silk, cellulose, chitin, etc. are composed of complex layered structures spanning micro-scale to macro-scale, and have been used for thousands of years; due to their excellent macroscopic properties, the applications of these materials have now been extended to different fields, such as biomedicine, photonics and electronics; however, research and exploration from the microstructure are still relatively limited; especially for natural silk, due to the high crystallinity and complex hierarchical structure, direct extraction of microstructures from natural silk Fiber remains a challenge
[0003] To date, the chemical methods of alkali or formic acid / NaCl dissolution and the physical method of ultrasonication have been reported, but both have inherent limitations; alkali or formic acid / NaCl or formic acid dissolution, these fiber bundles are only stable for a short period of time: 6 hours; for ultrasonic methods, silk fibers are only exfoliated to produce microfiber mats and lack reprocessing properties, thus limiting the application of the material; secondly, the two Neither method strips silk fibers on a single microfibril scale, which is critical in defining the mechanical properties of silk fibroin microfibers and can be used as a first step in regeneration of new materials; optimal silk fibroin microfibrils in aqueous solution The dispersion of the above-mentioned methods has not been well guaranteed, which limits the subsequent material processing
[0004] To sum up, the traditional method of directly extracting microfibril fibers from natural silk mainly has the following three major defects; first, the complete one-dimensional morphology of microfibrils cannot be preserved on the micron scale, nor can they be preserved for a long time; second , the stripped microfibers are difficult to further modify and process, and the dispersibility in aqueous solution is insufficient; third, the reagents used in the traditional method of stripping microfibers are not safe

Method used

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  • A preparation method of natural silk fibroin microfibers regulated by dopamine
  • A preparation method of natural silk fibroin microfibers regulated by dopamine
  • A preparation method of natural silk fibroin microfibers regulated by dopamine

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preparation example Construction

[0029] A preparation method of natural silk fibroin microfibers regulated by dopamine, comprising the following steps:

[0030] Step 1: removing sericin from silk to obtain degummed silk fibroin;

[0031] The process of removing sericin is as follows:

[0032] Disperse the silk in an alkaline solution with a pH of 7.5-10, the concentration of the silk is 0.001-0.5g / L; then treat it at 80°C-95°C for 20min-100min; repeat the same treatment 2-5 times, The degummed silk fibroin is obtained after drying; the drying temperature is 40°C-70°C, and the drying time is 2h-5h.

[0033] The alkaline solution is one of sodium carbonate solution, potassium carbonate solution, sodium bicarbonate solution, potassium bicarbonate solution, sodium hydroxide solution, and potassium hydroxide solution.

[0034] Step 2: Disperse dopamine in alkaline solution A, mix evenly and add the degummed silk fibroin obtained in step 1. The mass ratio of dopamine to degummed silk fibroin is 1:100-1:2, and pol...

Embodiment 1

[0041] A preparation method of natural silk fibroin microfibers regulated by dopamine, comprising the following steps:

[0042] Preparation of degummed silk fibroin: Weigh 0.2g of sodium carbonate and put it in a 25ml beaker, add 20ml of deionized water and 0.2g of silk, stir gently for 1min, after mixing evenly, place the beaker in a water pot at 90°C for 40min and heat treatment , after simply filtering to remove excess sodium carbonate solution, repeat the above operation 3 times, and finally take out the silk and put it into a petri dish with a diameter of 10mm, and then put it in a drying oven at 60°C to dry it, and then it will be removed. Silk fibroin after sericin.

[0043] Preparation of polydopamine-modified silk fibroin microfibers: Weigh 0.01g of sodium hydroxide and place it in a 25ml plastic beaker, add 10ml of deionized water and 0.2g of degummed silk fibroin, stir gently for 1min, and weigh 0.02g after mixing evenly. g of dopamine was added into the solution, ...

Embodiment 2

[0047] A preparation method of natural silk fibroin microfibers regulated by dopamine, comprising the following steps:

[0048] Preparation of degummed silk fibroin: Weigh 0.2g of sodium carbonate and put it in a 25ml beaker, add 20ml of deionized water and 0.2g of silk, stir gently for 1min, after mixing evenly, place the beaker in a water pot at 90°C for 40min and heat treatment , after simply filtering to remove excess sodium carbonate solution, repeat the above operation 3 times, and finally take out the silk and put it into a petri dish with a diameter of 10mm, and then put it in a drying oven at 60°C to dry it, and then it will be removed. Silk fibroin after sericin.

[0049]Preparation of natural silk fibroin microfibers: Weigh 0.8g of sodium hydroxide and place it in a 25ml plastic beaker, add 0.2g of degummed silk fibroin, stir gently for 1min and place it in a water bath at 60°C for 3h after being heated for 3h, then place the beaker After taking it out from the wat...

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Abstract

The invention discloses a preparation method of dopamine-regulated natural silk fibroin microfiber, and belongs to the technical field of biological materials. The preparation method comprises the following steps: firstly, heating a prepared mixed water solution of sodium carbonate and domestic silkworm silk to remove a sericin component from silkworm silk, performing washing and drying, and removing human immunogenic sericin to obtain a degummed fibroin material; adding the degummed fibroin material into a mixed solution of tri-aminomethane and dopamine; standing in a dark place to obtain a polydopamine-coated degummed fibroin material so as to protect the ordered one-dimensional structure of fiber; adding a prepared alkali solution, and heating to open a chained structure from a local part; then performing ultrasonic treatment on the solution for liquid stripping to obtain a stable one-dimensional natural silk fibroin microfiber material. By adopting the preparation method, the polydopamine-modified degummed fibroin material plays a role of regulating and controlling in the stripping process, so that high fiber dispersion is realized. The polydopamine-coated silk fibroin microfiber has superior biocompatibility, mechanical performance and degradability, the surface of the material is subjected to functional modification.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a method for preparing natural silk fibroin microfibers regulated by dopamine. Background technique [0002] Natural polymer materials such as silk, cellulose, chitin, etc. are composed of complex layered structures spanning the microscale to the macroscale, and have been used for thousands of years; due to their excellent macroscopic properties, the applications of these materials have now been extended to different fields, such as biomedicine, photonics and electronics; however, research and exploration from the microstructure are still relatively limited; especially for natural silk, due to the high crystallinity and complex hierarchical structure, direct extraction of microstructures from natural silk Fiber remains a challenge. [0003] To date, the chemical methods of alkali or formic acid / NaCl dissolution and the physical method of ultrasonication have been reported, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D06M15/61D06M11/76D06M11/38D06M101/12
CPCD06M11/38D06M11/76D06M15/61D06M2101/12
Inventor 鲁雄曾炎贾占荣
Owner SOUTHWEST JIAOTONG UNIV