Lactobacillus paracasei and application thereof
A Lactobacillus paracasei technology, applied to Lactobacillus paracasei and its application fields, can solve the problems of weak post-acidification characteristics of strains, complex fermented milk system, low survival rate of probiotics, etc., so as to reduce cholesterol level and maintain intestinal tract. The effect of micro-ecosystem balance, improvement of constipation and diarrhea problems
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Embodiment 1
[0037] 1. Material method
[0038] 1.1 Strains
[0039] 48 strains of Lactobacillus paracasei were randomly selected from free strains for screening. The experimental strains were isolated from healthy human intestines or traditional fermented foods. After 16S rDNA sequence analysis, all of them were Lactobacillus paracasei.
[0040] 1.2 Main reagents
[0041] Table 1 Main reagent information
[0042]
[0043] Physiological saline: 9.0g / L NaCl solution, sterilized at 121°C for 15min.
[0044] Phenolphthalein ethanol solution: 0.5g phenolphthalein / 100ml absolute ethanol, mix well and set aside.
[0045] 12% skim milk: weigh 12% skim milk powder, make up the remaining 88% with water, mix well, and sterilize at 121°C for 15 minutes.
[0046] MRS liquid medium: 48g MRS broth powder / 1L water, heat to boil to dissolve, sterilize at 121°C for 15min.
[0047] MRS solid medium: 63g MRS agar powder / 1L water, heat and boil to dissolve, sterilize at 121°C for 15 minutes, cool to 5...
Embodiment 2
[0109] The specific steps of making fermented milk are as follows:
[0110] (1) Activate strains in advance: activate the screened "weak acidifying bacteria", that is, a kind of Lactobacillus paracasei CG-C1, in a sterile environment, absorb the original strain of bacteria-preserving emulsion in MRS liquid medium, 37 ° C Cultured at a constant temperature for 12 hours, activated, and activated continuously for three generations. Carry out Gram staining and microscopic examination to the third-generation working bacteria, place the qualified bacteria in a 4°C refrigerator for temporary storage, and activate with 2% inoculum to obtain 100ml of the third-generation working bacteria. After passing the microscopic examination, it was stored in a 4°C refrigerator for later use.
[0111] (2) Calculating the amount of raw materials: Calculate the required amount of raw materials according to the ratio of 3% protein, 32.9% skim milk powder, and 7% sucrose.
[0112] (3) Raw material p...
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