Functional microbial fermented feed, and production method and application thereof
A technology of functional microorganisms and fermented feed, which is applied in the fields of animal nutrition and microbial fermentation to achieve the effect of promoting growth, improving the breeding environment and improving the attractiveness of food.
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Embodiment 1
[0115] (1) Determination of color, smell and inhibition zone in the formula combination of fulvic acid powder, Phellodendron chinensis powder, Coptis chinensis powder, Scutellaria baicalensis powder, Astragalus root powder and Radix isatidis powder:
[0116] 5-15 parts by weight of fulvic acid powder, 1-3 parts by weight of Phellodendron sichuanensis powder, 1-3 parts by weight of Coptis chinensis powder, 1-3 parts by weight of Scutellaria baicalensis powder, 1-3 parts by weight of Radix Astragali powder, 1-3 parts by weight of Radix Radix Radix parts by weight, take it out and set aside.
[0117] Grind the fulvic acid powder, Phellodendron sichuanensis powder, Coptis chinensis powder, Scutellaria baicalensis powder, Astragalus root powder, Radix Radix Radix powder into 100 mesh respectively, and mix them evenly according to the proportion for later use.
[0118] Put 1 gram of powder into 1000ML of water for each combination, that is, dilute 1000 times, then boil and place for...
Embodiment 2
[0126] The formulation of Bacillus subtilis fermenter seed medium formula and confirmation of its fermentation conditions
[0127] Bacillus subtilis (Bacillus subtilis) bacterial microorganism (mainly decomposes proteins to synthesize amino acids and peptides). The specific operation steps of single colony purification and optimization, solid eggplant-shaped flat bottle seed culture and fermenter tank seed culture are as follows. Bacillus subtilis has fast propagation speed, high temperature resistance, acid and alkali resistance, extrusion resistance, good stability and wide application range, etc. features.
[0128] Single colony isolation: spread the activated Bacillus subtilis slant strain on a petri dish filled with newly prepared glucose beef extract peptone medium, and cultivate it in a constant temperature incubator at 30°C for 24 hours to obtain a single colony of Bacillus subtilis. Colony seeds;
[0129] Solid eggplant-shaped flat bottle seed culture: Bacillus subt...
Embodiment 3
[0143] Experiments on the optimal ratio of fermentation raw materials and control of fermentation conditions in the solid state fermentation process:
[0144] In terms of parts by weight, the fermentation raw materials are composed of: 10-30 parts by weight of soybean meal, 5-15 parts by weight of fulvic acid powder, 10-30 parts by weight of corn steep liquor powder, 10-30 parts by weight of wheat bran, and 10-30 parts by weight of rice bran powder. Parts by weight, 1 to 3 parts by weight of Phellodendron Phellodendri powder, 1 to 3 parts by weight of Rhizoma Coptidis Rhizome powder, 1 to 3 parts by weight of Scutellaria baicalensis powder, 1 to 3 parts by weight of Radix Astragali powder, and 1 to 3 parts by weight of Radix Radix Radix powder;
[0145] The weight composition of beneficial microbial flora is as follows: 30-40 parts by weight of Bacillus subtilis seed liquid, 30-40 parts by weight of Bacillus licheniformis seed liquid, 20-30 parts by weight of Lactococcus lactis...
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