A kind of method for irradiating fresh-keeping Cordyceps sinensis
A technology for fresh Cordyceps sinensis and Cordyceps sinensis is applied in the field of preservation of Cordyceps sinensis, which can solve the problems of complicated operation and high equipment dependence, and achieve the effects of reducing the growth rate of polyphenol oxidase activity and the decreasing rate of soluble protein content.
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Embodiment 1
[0063] At 0 ° C, the head, subtots and worms collected by the mud in the origin were cleaned in the head, subtots and worms collected by the mud, and the fresh winter insects after cleaning was placed in a mass of 0.1% in the calcium lactate solution. Ultrasonic cleaning 5 times (power 250W, frequency 120 Hz, temperature 0 ° C, time 5 / time), 0. ° C drained surface moisture.
[0064] Preparation of preservative: 3 mmol / l sorbitol, 3 mmol / lvc, 6 μmol / L plant acid, L-cysteine concentration of 0.4 mg / kg, 30 mg / mg Ethanol extract, 50mg / L shell Sugar, 100mg / L Lactic Acid Streptococcus Antibacterial Peptide (Nisin, Manufacturer: Shandong Freda Biotechnology Co., Ltd.).
[0065] Preparation of ethanol extract of Cordyceps sinensis fermentation: Costiocyterami cultured in liquid fermentation medium (including potato, soy, sucrose, glucose, protein, yeast cream, mineral salt and vitamin), 26 ° C culture 7d, The supernatant was centrifuged to obtain a fermentation of Cordyc...
Embodiment 2
[0069] At 10 ° C, the head, subtots and worms collected by the mud with linear water were cleaned in sequential chili-chords, and the fresh winter worms after cleaning were placed in a mass percentage concentration of 0.5%. Ultrasonic cleaning 1 time (power 1200W, frequency 40 kHz, temperature 10 ° C, time 5 min / time), 10 ° C drained surface moisture.
[0070] Preparation of preservative: 1 mmol / l sorbitol, 1 mmol / L is also Vc, 9 μmol / L plant acid, L-cysteine concentration of 0.8 mg / kg, 100 mg / ml Cordyceps extract, 200mg / L Shell oligosaccharide, 200 mg / l Streptomyxide antibacterial peptide (ε-polylysine, ε-polylysine, manufacturer: Zhengzhou Bai Na Buddha Biological Engineering Co., Ltd.);
[0071] Preparation of ethanol extract of Cordyceps Summer Grass Fermentation Liquid: Turning Cordyceps Separami is activated in liquid fermentation medium (including potato, soybeans, sucrose, glucose, protein, yeast cream, mineral salt and vitamin), 7d at 24 ° C, The superna...
Embodiment 3
[0075] At 5 ℃, the origin of the linear water collected with a soil Cordyceps head, child seat, and the mixture was washed worms; Cordyceps cleaned fresh mass percent was placed 0.5% calcium lactate solution ultrasonic cleaning three times (power 600W, frequency 80Hz, temperature between 5 ℃, time 7min / times), 5 ℃ drain surface moisture.
[0076] Preservation solution preparation: 2mmol / L sorbitol, 2mmol / L iso Vc, 8μmol / L phytic acid, L- cysteine concentration of 0.6mg / kg, 60mg / mL of fermentation broth sinensis ethanol extract, 150mg / L chitooligosaccharide, 150mg / L Pediococcus Peptide (PediocinPA-1, to the Pediococcus acidilactici P.acidilactici, Pediococcus beer P.cerevisicae, Pediococcus pentosaceus Pediococcus sp P.pentosaceus like broth).
[0077] Preparation of ethanol extract of Cordyceps sinensis fermentation broth: After activation Cordyceps strain was inoculated in a liquid fermentation medium (including potato, soybean, sucrose, glucose, peptone, yeast ...
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