UTMD-based dual drug-loading nano platform and preparation method and application thereof
A dual-drug-loading and nano-technology, which is applied in the direction of pharmaceutical formulations, preparations for in vivo tests, and medical preparations with non-active ingredients, can solve the problem of not finding dendrimer binding carbon points and loading anticancer drugs, etc. problem, to achieve the effect of inhibiting tumor growth, reducing the level of ATP, and inhibiting MDR
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Embodiment 1
[0061] (1) Dissolve 0.2g of 4-amino ammonium salicylate in 10mL of ultrapure water, then transfer the mixture to a polytetrafluoroethylene reactor for heating reaction, the reaction temperature is 180°C, and the reaction time is 3 hours , the reaction system was naturally cooled to room temperature, centrifuged for 20 minutes (4000rpm) to remove excessively carbonized particles, then filtered through a microporous membrane with a pore size of 220 nm, and finally freeze-dried to obtain yellow carbon dot y-CDs.
[0062] (2) Add CDI solution (43.7mg, 15mL DMSO) to TPGS solution (17.73mg, 6mL DMSO), stir at room temperature for 6 hours, then add G5 (15.24mg, 7mL DMSO) solution dropwise to the solution, and stir to react 3 day, and then use a dialysis bag with a molecular weight cut-off of 5000 to dialyze the aqueous solution (2 L / time, 3 times / day), and freeze-dry to obtain G5-TPGS.
[0063] (3) Add y-CDs solution (4.54mg, 4mL) to G5-TPGS solution (13.62mg, 7mL), stir at room temp...
Embodiment 2
[0066] Get the y-CDs prepared in Example 1 to be dissolved in ultrapure water, and measure the ultraviolet-visible light spectrum and the fluorescence excitation spectrum of the y-CDs aqueous solution as shown in figure 2 As shown in a, it can be observed that there is a characteristic peak of y-CDs at 282nm, and the maximum excitation wavelength of y-CDs is located at 490nm. The emission spectra of y-CDs at different excitation wavelengths are as follows: figure 2 As shown in b, it can be observed that the maximum emission wavelength of y-CDs is 547 nm, and the emission wavelength does not change with the excitation wavelength. The fluorescence lifetime spectrum of y-CDs is as follows figure 2 As shown in c, it can be seen that the fluorescence lifetime of y-CDs is 2.31 ns under the conditions of excitation wavelength of 490 nm and emission wavelength of 536 nm. The surface functional groups of y-CDs were studied by Fourier transform (FTIR) spectroscopy, and the infrared...
Embodiment 3
[0074] Dissolve the (G5-TPGS@y-CDs)-DOX prepared in Example 1 with pH=7.4 and pH=5.5 buffers respectively to prepare a solution with a concentration of 1mg / mL, take 1mL into a dialysis bag for fixation, and place Place in a container containing 9mL of buffer solution of different pH and shake in a shaker at 37°C. Samples were taken at different time points. Take 1mL of the liquid outside the dialysis bag each time, measure its absorbance at 480nm, and then add 1mL of the corresponding buffer solution to the outside of the dialysis bag. This method was used to obtain the kinetic curves of DOX released from (G5-TPGS@y-CDs)-DOX under different pH conditions in vitro, such as Figure 7 As shown, in 72 hours, the release amount of DOX in a weakly acidic environment (pH=5.5) was 52.11%, which was greater than 36.85% released in a physiological environment (pH=7.4). It shows that this drug-loading system has a certain pH responsiveness, and the release rate in the weakly acidic env...
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