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UTMD-based dual drug-loading nano platform and preparation method and application thereof

A dual-drug-loading and nano-technology, which is applied in the direction of pharmaceutical formulations, preparations for in vivo tests, and medical preparations with non-active ingredients, can solve the problem of not finding dendrimer binding carbon points and loading anticancer drugs, etc. problem, to achieve the effect of inhibiting tumor growth, reducing the level of ATP, and inhibiting MDR

Inactive Publication Date: 2019-05-31
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are no domestic and foreign literatures and patents about dendrimers combined with carbon dots and loaded with anticancer drugs and combined with UTMD technology

Method used

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  • UTMD-based dual drug-loading nano platform and preparation method and application thereof
  • UTMD-based dual drug-loading nano platform and preparation method and application thereof
  • UTMD-based dual drug-loading nano platform and preparation method and application thereof

Examples

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Embodiment 1

[0061] (1) Dissolve 0.2g of 4-amino ammonium salicylate in 10mL of ultrapure water, then transfer the mixture to a polytetrafluoroethylene reactor for heating reaction, the reaction temperature is 180°C, and the reaction time is 3 hours , the reaction system was naturally cooled to room temperature, centrifuged for 20 minutes (4000rpm) to remove excessively carbonized particles, then filtered through a microporous membrane with a pore size of 220 nm, and finally freeze-dried to obtain yellow carbon dot y-CDs.

[0062] (2) Add CDI solution (43.7mg, 15mL DMSO) to TPGS solution (17.73mg, 6mL DMSO), stir at room temperature for 6 hours, then add G5 (15.24mg, 7mL DMSO) solution dropwise to the solution, and stir to react 3 day, and then use a dialysis bag with a molecular weight cut-off of 5000 to dialyze the aqueous solution (2 L / time, 3 times / day), and freeze-dry to obtain G5-TPGS.

[0063] (3) Add y-CDs solution (4.54mg, 4mL) to G5-TPGS solution (13.62mg, 7mL), stir at room temp...

Embodiment 2

[0066] Get the y-CDs prepared in Example 1 to be dissolved in ultrapure water, and measure the ultraviolet-visible light spectrum and the fluorescence excitation spectrum of the y-CDs aqueous solution as shown in figure 2 As shown in a, it can be observed that there is a characteristic peak of y-CDs at 282nm, and the maximum excitation wavelength of y-CDs is located at 490nm. The emission spectra of y-CDs at different excitation wavelengths are as follows: figure 2 As shown in b, it can be observed that the maximum emission wavelength of y-CDs is 547 nm, and the emission wavelength does not change with the excitation wavelength. The fluorescence lifetime spectrum of y-CDs is as follows figure 2 As shown in c, it can be seen that the fluorescence lifetime of y-CDs is 2.31 ns under the conditions of excitation wavelength of 490 nm and emission wavelength of 536 nm. The surface functional groups of y-CDs were studied by Fourier transform (FTIR) spectroscopy, and the infrared...

Embodiment 3

[0074] Dissolve the (G5-TPGS@y-CDs)-DOX prepared in Example 1 with pH=7.4 and pH=5.5 buffers respectively to prepare a solution with a concentration of 1mg / mL, take 1mL into a dialysis bag for fixation, and place Place in a container containing 9mL of buffer solution of different pH and shake in a shaker at 37°C. Samples were taken at different time points. Take 1mL of the liquid outside the dialysis bag each time, measure its absorbance at 480nm, and then add 1mL of the corresponding buffer solution to the outside of the dialysis bag. This method was used to obtain the kinetic curves of DOX released from (G5-TPGS@y-CDs)-DOX under different pH conditions in vitro, such as Figure 7 As shown, in 72 hours, the release amount of DOX in a weakly acidic environment (pH=5.5) was 52.11%, which was greater than 36.85% released in a physiological environment (pH=7.4). It shows that this drug-loading system has a certain pH responsiveness, and the release rate in the weakly acidic env...

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Abstract

The invention relates to a UTMD-based dual drug-loading nano platform and a preparation method and application thereof. The method comprises synthesizing carbon dots y-CDs by using a one-step hydrothermal method; connecting an anticancer drug TPGS on a dendrimer at the amino terminal to obtain G5-TPGS; combining G5-TPGS with y-CDs by non-covalent interaction to form a nano hybrid material G5-TPGS@y-CDs; loading an anticancer drug doxorubicin DOX to obtain (G5-TPGS@y-CDs)-DOX and then combining the UTMD technology. The platform is simple, easy to operate and separate, low in cost and commercialized in raw material source, and has good development prospects.

Description

technical field [0001] The invention belongs to the technical field of functional hybrid nanomaterials, in particular to a dendrimer / carbon dot double-loaded drug nano platform enhanced based on UTMD technology and its preparation method and application. Background technique [0002] As a kind of optical imaging, fluorescence imaging has the advantages of high temporal / spatial resolution, high soft tissue contrast, imaging contrast and correlation with biomolecules, rich information, suitable for multi-parameter composite measurement, and low price. in the diagnosis of tumors. Carbon dots (CDs), as a new type of biological imaging quantum dots, can be used for fluorescence imaging. Due to its excellent characteristics such as low toxicity, good biocompatibility, good water solubility, high stability, and high fluorescence tunability, it has a good application prospect in fluorescence imaging (Wang W, et al., Sci. China Chem. 2014, 57(4):522-539). It has been reported that...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K47/59A61K47/52A61K31/704A61K31/355A61P35/00G01N21/64
Inventor 史向阳李丹范钰林丽洲杜联芳
Owner DONGHUA UNIV
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