Method for evaluating effect of PM2.5 particles on free radical metabolic pathways in human lung cells

A technology based on free radicals and lung epithelial cells, applied in the field of evaluation based on molecular biology and analytical chemistry, can solve the problems of abnormal transcription, reduced activity, and reduced activity of antioxidant enzyme systems, achieving good specificity, small sample size, The effect of reducing the difference

Inactive Publication Date: 2019-06-04
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to small molecule metabolites involved in intracellular energy metabolism, enzymes that regulate various energy metabolism pathways also play an important role, PM 2.5 The free radicals produced by exposure act as functional signaling molecules, triggering the translocation of nuclear transcription factors to the nucleus, resulting in abnormal transcription of the antioxidant enzyme system, causing key enzymes regulating gl

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for evaluating effect of PM2.5 particles on free radical metabolic pathways in human lung cells
  • Method for evaluating effect of PM2.5 particles on free radical metabolic pathways in human lung cells
  • Method for evaluating effect of PM2.5 particles on free radical metabolic pathways in human lung cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The PM2.5 particles described in this example were taken from the urban area of ​​Dalian City.

[0025] Example: PM 2.5 Effects of full chemical composition particle exposure on free radical metabolism in human lung epithelial cells

[0026] First, culture and expose human lung epithelial cells, the steps are as follows:

[0027] Human lung epithelial cells were seeded on a six-well plate at a seeding density of 1×10 5 cells / mL, the volume of the culture medium in each well of the six-well plate is 3mL, the culture medium is serum-free, and added: bovine pituitary extract 2ml / 500ml, hydrocortisone 0.5ml / 500mL human epidermal growth factor 0.5ml / 500ml, adrenal gland Insulin 0.5ml / 500ml, Insulin 0.5ml / 500ml, Transferrin 0.5ml / 500ml, Iodothyronine 0.5ml / 500ml, Retinoic Acid 0.5ml / 500ml and Gentamicin / Aphotericin-B 0.5m / 500ml, to simulate the normal growth conditions of cells. Cultivate in the incubator for 24h (37°C, 5% CO 2 ), when the cells entered the logarithmic ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for evaluating an effect of PM2.5 full chemical component particles on free radical metabolic pathways in human lung cells. The method is characterized in that human lung-bronchial epithelial cells are exposed by means of the PM2.5 full chemical component particles, a liquid chromatography-mass spectrometry (LC- MS) can be used for effectively analyzing micromolecular metabolites in cells, enzymatic activity is quickly analyzed by combining with a biological detection method, and changes of the micromolecular metabolites related associated with intracellular free radical metabolism and activity changes of a rate-limiting enzyme that plays a key role in energy metabolism are integrated, in summary, a set of new methods for evaluating the interference on intracellular oxygen free radical metabolism after cells are exposed to toxic effect of exogenous pollution are constructed based on molecular biology. The method can analyze the influence of contaminantson the free radical metabolic pathways in the cells intuitively, and has the advantages of simple preprocessing method, rapid detection, good repeatability, comprehensive information acquisition andthe like.

Description

technical field [0001] The invention belongs to the field of atmospheric particle toxicity evaluation, and relates to an evaluation method based on molecular biology and analytical chemistry, which evaluates the cytotoxicity of small doses of environmental pollutants through the change of atmospheric particles on cellular energy metabolism pathways. Background technique [0002] Atmospheric fine particles (Atmospheric fine particles) have a complex composition, with an aerodynamic diameter of less than 2.5 μm particles (PM 2.5 , Particulate matter that can enter the lungs) is the core focused substance of this patent, which has the characteristics of long-distance environmental migration, environmental persistence, and bioaccumulation. PM 2.5 The particle components are complex and heterogeneous mixtures. The particles themselves belong to the class of insoluble particles, and there are large variable factors in time and space. The components contain many different chemical...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/50G01N33/53
Inventor 宋肖垚陈吉平耿柠波张海军张亦弛
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap