Method for evaluating effect of PM2.5 particles on free radical metabolic pathways in human lung cells

Abstract

The invention discloses a method for evaluating an effect of PM2.5 full chemical component particles on free radical metabolic pathways in human lung cells. The method is characterized in that human lung-bronchial epithelial cells are exposed by means of the PM2.5 full chemical component particles, a liquid chromatography-mass spectrometry (LC- MS) can be used for effectively analyzing micromolecular metabolites in cells, enzymatic activity is quickly analyzed by combining with a biological detection method, and changes of the micromolecular metabolites related associated with intracellular free radical metabolism and activity changes of a rate-limiting enzyme that plays a key role in energy metabolism are integrated, in summary, a set of new methods for evaluating the interference on intracellular oxygen free radical metabolism after cells are exposed to toxic effect of exogenous pollution are constructed based on molecular biology. The method can analyze the influence of contaminantson the free radical metabolic pathways in the cells intuitively, and has the advantages of simple preprocessing method, rapid detection, good repeatability, comprehensive information acquisition andthe like.

Description

technical field [0001] The invention belongs to the field of atmospheric particle toxicity evaluation, and relates to an evaluation method based on molecular biology and analytical chemistry, which evaluates the cytotoxicity of small doses of environmental pollutants through the change of atmospheric particles on cellular energy metabolism pathways. Background technique [0002] Atmospheric fine particles (Atmospheric fine particles) have a complex composition, with an aerodynamic diameter of less than 2.5 μm particles (PM 2.5 , Particulate matter that can enter the lungs) is the core focused substance of this patent, which has the characteristics of long-distance environmental migration, environmental persistence, and bioaccumulation. PM 2.5 The particle components are complex and heterogeneous mixtures. The particles themselves belong to the class of insoluble particles, and there are large variable factors in time and space. The components contain many different chemical...

AI Technical Summary

Problems solved by technology

In addition to small molecule metabolites involved in intracellular energy metabolism, enzymes that regulate various energy metabolism pathways also play an important role, PM 2.5 The free radicals produced by exposure act as functional signaling molecules, triggering the translocation of nuclear transcription factors to the nucleus, resulting in abnormal transcription of the antioxidant enzyme system, causing key enzymes regulating glycolysis including hexokinase, phosphofructokinase and pyruvate kinase; Changes in the expression and activity of catalase, superoxide dismutase, and glutathione peroxidase can also cause abnormal activity of peroxidase and heme oxidase and glutathione metabolic enzymes such as glucose- The activities of 6-phosphate dehydrogenase, glutathione reductase, glutathione peroxidase and protein tyrosine phosphatase were significantly reduced

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