High purity soybean oligopeptide having unique solubility and preparation method and application

A soybean oligopeptide, high-purity technology, applied in the field of deep processing of soybean protein, can solve the problems of strong hygroscopicity, poor sensory perception, and insignificant efficacy, so as to improve product yield, ensure high efficiency, and compact preparation process Effect

Inactive Publication Date: 2019-06-07
广州合诚实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether soybean meal is used as a raw material or protein isolate is used as a raw material to produce soybean peptides, there are the following problems: (1) the protein purity is low, and the protein content of soybean peptide products reaching the first-class standard stipulated in GB/T 22492 is greater than or equal to 90%. Dry basis), high ash content (on dry basis, GB/T 22492 first-level standard requires ≤6.5%, high sodium salt content, some people such as hypertensive people are restricted in eating), and contain a small amount of carbohydrates Compound (ranging from 1% to 3%), strong hygroscopicity and easy to be affected by moisture; (2) The content of peptides and oligopeptides is low, and GB/T22492 stipulates that the content of peptides (calculated on a dry basis) is ≥55%, ≥80% peptides A segment with a relative

Method used

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  • High purity soybean oligopeptide having unique solubility and preparation method and application
  • High purity soybean oligopeptide having unique solubility and preparation method and application
  • High purity soybean oligopeptide having unique solubility and preparation method and application

Examples

Experimental program
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Example Embodiment

[0055] Example 1

[0056] (1) Dispersion and hydration of soy protein isolate powder: Use deionized water to prepare soy protein isolate powder into a protein slurry with a mass percentage of 10%, and use a mixer to shear and disperse the soy protein powder evenly and hydrate at a speed of 2500 rpm. 15min;

[0057] (2) Enzymatic hydrolysis: adjust the pH value of the protein slurry to 9.0 with a sodium hydroxide solution with a concentration of 3mol / L, and add Alcalase 2.4L (commercial enzyme from Novozymes Biotechnology Co., Ltd.) equivalent to 1.0% of the protein powder mass Stir at 53°C (stirring speed is 36rpm) for 6.5h to hydrolyze, then add Neutrase 0.8L (commercial enzyme from Novozymes) equivalent to 0.6% of protein powder mass, 480L type α at medium temperature equivalent to 0.6% of protein powder mass -Amylase (commercial enzyme from Novozymes Biotechnology Co., Ltd.), stirred and hydrolyzed at 53°C for 1.5h;

[0058] (3) Enzyme inactivation and activated carbon adsorptio...

Example Embodiment

[0062] Example 2

[0063] (1) Dispersion and hydration of soy protein isolate powder: Use deionized water to prepare soy protein isolate powder into a protein slurry with a mass percentage of 5%, and use a mixer to shear and disperse the soy protein powder evenly and hydrate at a speed of 2000 rpm. 20min;

[0064] (2) Enzymatic hydrolysis: adjust the pH value of the protein slurry to 8.0 with sodium hydroxide solution with a concentration of 1mol / L, and add Alcalase 2.4L (commercial enzyme from Novozymes Biotechnology Co., Ltd.) equivalent to 0.5% of the protein powder mass Stir at 50℃ (stirring speed is 36rpm) and hydrolyze for 10h, then add Neutrase 0.8L (commercial enzyme from Novozymes) equivalent to 0.2% of protein powder mass, medium temperature 480L α-type equivalent to 0.2% of protein powder mass Amylase (commercial enzyme from Novozymes Biotechnology Co., Ltd.) was stirred and hydrolyzed at 50°C for 2h;

[0065] (3) Enzyme inactivation and activated carbon adsorption and d...

Example Embodiment

[0069] Example 3

[0070] (1) Dispersion and hydration of soy protein isolate powder: Use deionized water to formulate soy protein isolate powder into a protein slurry with a mass percentage of 15%. Use a mixer to shear and disperse the soy protein powder evenly and hydrate at a speed of 3000 rpm. 10min;

[0071] (2) Enzymatic hydrolysis: adjust the pH value of the protein slurry to 10.0 with sodium hydroxide solution with a concentration of 5mol / L, and add Alcalase 2.4L (commercial enzyme from Novozymes Biotechnology Co., Ltd.) equivalent to 1.0% of the mass of protein powder. Stir at 55℃ (stirring speed is 36rpm) to hydrolyze for 3h, then add Neutrase 0.8L (commercial enzyme from Novozymes Biotechnology Co., Ltd.) equivalent to 1.0% of the mass of protein powder, 1.0% medium temperature 480L α-amylase (Novi (Commercial enzyme from Xin Biotechnology Co., Ltd.) was stirred and hydrolyzed at 55°C for 1h;

[0072] (3) Enzyme inactivation and activated carbon adsorption and decoloriza...

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Abstract

The invention discloses a high purity soybean oligopeptide having unique solubility and a preparation method and application. Soy protein isolate powder is subjected to hydration, enzymolysis, enzymedeactivation, charcoal absorption for decoloring, and solid-liquid separation to obtain liquid, and the obtained liquid is subjected to ultrafiltration, nanofiltration, concentration, sterilization, and drying, so that the high purity soybean oligopeptide having unique solubility is obtained. The protein content of the oligopeptide is greater than or equal to 94%, the content of peptide is greaterthan or equal to 91%, the peptide of which the relative molecular weight is smaller than 1000Da is 83.7%-85.6%, free amino acid is 0.32%-0.41%, and the sodium content is 48.7-56.6mg/100g. In the dissolving process, high sensory experience of being free from aggregation, enabling peptide powder to directly and quickly dissolve in the surface of an aqueous solution like snowslide, being free from bubbles, and the like is presented in the dissolving process; the high purity soybean oligopeptide is free from peculiar smell, free from bitterness, and soft in mouth feel, can be used as superior rawmaterials of health-care foods, special medical foods and common foods, and is suitable for various crowds.

Description

technical field [0001] The invention relates to a deep processing technology of soybean protein, in particular to a high-purity soybean oligopeptide with unique solubility, its preparation method and application. Background technique [0002] Soybean peptide is the product of soybean protein produced by enzymatic hydrolysis or microbial fermentation. Compared with soybean protein, it is easy to absorb, can quickly replenish the body's energy, has low allergenicity, strong heat resistance, acid resistance, low solution viscosity, and good emulsification. In addition, it has the characteristics of enhancing immunity, anti-fatigue, anti- Oxidation, lowering blood pressure, lowering blood fat, lowering cholesterol, promoting fat metabolism, promoting mineral absorption and other unique physiological effects. Therefore, soybean peptide has become a new and most potential deep-processing product of soybean protein, and has very broad prospects in the fields of functional food (es...

Claims

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Application Information

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IPC IPC(8): A23J3/16A23J3/34A23L33/18
Inventor 周雪松曾建新蒋文真
Owner 广州合诚实业有限公司
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