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Method of simultaneously detecting dopamine and uric acid based on poly (L-glutathione) modified glassy carbon electrode

A glassy carbon electrode and glutathione technology, which is applied in the field of electrochemical analysis and detection, can solve the problems of complicated preparation steps, narrow linear range and high detection limit, and achieves good repeatability, less drug dosage, and simple and fast preparation method. Effect

Active Publication Date: 2019-07-02
NANJING UNIV OF SCI & TECH
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Problems solved by technology

For example, Xu et al. prepared a platinum-titanium alloy-modified glassy carbon electrode by a two-step dealloying method, and realized the simultaneous detection of DA and UA (Zhao, D., et al, 2016. A highly sensitive and stable electrochemical sensor for simultaneous detection towards ascorbic acid, dopamine, and uricacid based on the hierarchical nanoporous PtTi alloy. Biosens Bioelectron 82, 119-126.), but the preparation steps of this method are cumbersome and the detection limit is high
Ma et al prepared a 3D graphene hydrogel / gold nanoparticle composite modified glassy carbon electrode for the simultaneous determination of DA and UA (Zhu, Q., et al, 2017. 3D graphenehydrogel–gold nanoparticles nanocomposite modified glassy carbon electrode for the simultaneous determination of ascorbic acid, dopamine and uric acid. Sens. Actuators B: Chem, 238, 1316-1323.), but the method has a narrow linear range for DA and UA, which are 0.2-30μM and 1-60μM, respectively

Method used

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  • Method of simultaneously detecting dopamine and uric acid based on poly (L-glutathione) modified glassy carbon electrode
  • Method of simultaneously detecting dopamine and uric acid based on poly (L-glutathione) modified glassy carbon electrode
  • Method of simultaneously detecting dopamine and uric acid based on poly (L-glutathione) modified glassy carbon electrode

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Experimental program
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Effect test

Embodiment 1

[0026] (1) Use α-A1 of 1.0, 0.3, and 0.05 μm respectively 2 o 3 Polish the glassy carbon electrode, ultrasonically clean it with deionized water after each polishing, and finally dry it with high-purity nitrogen;

[0027] (2) Immerse the glassy carbon electrode in a solution containing 10mM L -In the electrolytic cell of glutathione phosphate buffer solution, deoxygenate with nitrogen;

[0028] (3) Electrochemical polymerization was carried out by cyclic voltammetry, and a three-electrode system was used. The glassy carbon electrode was used as the working electrode, the platinum sheet electrode was used as the counter electrode, and the saturated calomel electrode was used as the reference electrode. The low potential was -1.5V, and the high potential was 2.0V, sweep speed is 100mVs -1 , scanning 12 circles, the obtained modified electrode was rinsed with deionized water, and dried with nitrogen to obtain poly L - Glutathione modified electrodes.

[0029] (4) will gather...

Embodiment 2

[0031] Polymerization in the presence of uric acid L -Glutathione-modified glassy carbon electrode for dopamine detection.

[0032] (1) Use α-A1 of 1.0, 0.3, and 0.05 μm respectively 2 o 3 Polish the glassy carbon electrode, ultrasonically clean it with deionized water after each polishing, and finally dry it with high-purity nitrogen;

[0033] (2) Immerse the glassy carbon electrode in a solution containing 10mM L -In the electrolytic cell of glutathione phosphate buffer solution, deoxygenate with nitrogen;

[0034] (3) Electrochemical polymerization was carried out by cyclic voltammetry, and a three-electrode system was used. The glassy carbon electrode was used as the working electrode, the platinum sheet electrode was used as the counter electrode, and the saturated calomel electrode was used as the reference electrode. The low potential was -1.5V, and the high potential was 2.0V, sweep speed is 100mVs -1 , scanning 12 circles, the obtained modified electrode was rins...

Embodiment 3

[0037] dopamine in the presence of L - Glutathione-modified glassy carbon electrode for the detection of uric acid.

[0038] (1) Use α-A1 of 1.0, 0.3, and 0.05 μm respectively 2 O 3 Polish the glassy carbon electrode, ultrasonically clean it with deionized water after each polishing, and finally dry it with high-purity nitrogen;

[0039] (2) Immerse the glassy carbon electrode in 10 mM L - In the electrolytic cell of glutathione phosphate buffer solution, nitrogen is used to remove oxygen;

[0040] (3) Electrochemical polymerization is carried out by cyclic voltammetry, using a three-electrode system, the glassy carbon electrode is the working electrode, the platinum sheet electrode is the counter electrode, the saturated calomel electrode is the reference electrode, the low potential is -1.5V, and the high potential is -1.5V. 2.0V, sweep speed is 100mVs -1 , scanning for 12 times, the obtained modified electrode was rinsed with deionized water, and blown dry with nitroge...

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Abstract

The invention discloses a method of simultaneously detecting dopamine and uric acid based on a poly (L-glutathione) modified glassy carbon electrode. The method firstly immerses a clean glassy carbonelectrode in an L-glutathione phosphate buffer, nitrogen is supplied for deoxidization, cyclic voltammetry is adopted for electrochemical polymerization, a poly (L-glutathione) modified electrode is obtained, the poly (L-glutathione) modified electrode is then placed in a phosphate buffer of a to-be-detected sample, differential pulse voltammetry is adopted, electrochemical response current is detected, and according to the linear relationship between the response current and a dopamine concentration and the linear relationship between the response current and a uric acid concentration, the concentration of dopamine and the concentration of uric acid in the to-be-detected sample are calculated and obtained. The method disclosed in the invention has the advantages of high sensitivity, simple operation, good stability, good repeatability and the like, the linear detection ranges of dopamine and uric acid are 2 to 300 muM and 10 to 500 muM respectively, and practical application prospectsare achieved in clinical detection of dopamine and uric acid.

Description

technical field [0001] The invention belongs to the technical field of electrochemical analysis and detection, in particular to a polymer-based L - A method for simultaneously detecting dopamine and uric acid at a glutathione-modified glassy carbon electrode. Background technique [0002] Dopamine (DA) is an important neurotransmitter, which plays a role in regulating physiological and cognitive functions in the human body. Monitoring the real-time signal changes of biological dopamine is of great significance for discovering its function in the human body and further understanding the function of the brain. Uric acid (UA) is one of the end products of purine metabolism in the human body, and its concentration changes in body fluids can reflect the status of immune and metabolic functions in the human body. DA and UA usually coexist in body fluids (such as blood and urine), and some diseases such as gout, epilepsy, Parkinson's disease, etc. can be judged by their content i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/308G01N27/3277
Inventor 唐卫华闵梦珂唐键周杰
Owner NANJING UNIV OF SCI & TECH
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