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Method and sampling device for separating and purifying water body micro-organism strains

A technology of microbial strains and collection devices, which is applied in biochemical equipment and methods, biological material sampling methods, and separation of microorganisms. problems, to achieve the effect of easy removal, not easy to decline microbial metabolic function, and small footprint of equipment

Active Publication Date: 2019-07-19
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the purification of microbial strains, the existing methods mainly include ten-fold dilution separation method, plate streaking method, plate coating method, puncture separation method, single cell separation method and ultramicro membrane separation method, etc., but these methods often The process is fine and the procedures are cumbersome, especially the operator is required to have a very high level of operation, which will not only greatly reduce the purification efficiency of pure microbial strains, but also cause the loss of the activity of some microbial strains

Method used

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  • Method and sampling device for separating and purifying water body micro-organism strains
  • Method and sampling device for separating and purifying water body micro-organism strains
  • Method and sampling device for separating and purifying water body micro-organism strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Step 1, the NH of 1.0g / L 4 Cl, 0.3g / L of K 2 HPO 4 , 0.3g / L of KH 2 PO 4 , 0.1g / L KCl, 0.1g / L CaCl 2 2H 2 The NaCl of 0,30g / L, the yeast extract of 0.1g / L, the cysteine ​​of 0.5g / L, the sodium thiosulfate of 3.16g / L and 1ml trace element nutrient solution are mixed to obtain the culture medium solution of 1L, Wherein the trace element nutrient solution contains copper, manganese, zinc, iron, magnesium, molybdenum and boron, and the culture medium solution is adjusted to pH 7.6 with 10M KOH solution. In terms of the percentage of volume in milliliters, add 0.5% agar powder, heat and dissolve to prepare a solution;

[0057] Step 2, uniformly disperse polyvinyl alcohol fibers with a length of 10 mm in 5% NaOH solution, stir at 50° C. for 12 hours, collect the fibers and dry them;

[0058] Step 3, according to the ratio of the quality of the modified polyvinyl alcohol fiber silk to the mass of the BM solid medium solution, uniformly mix 1% of the modified polyvinyl a...

Embodiment 2

[0068] Step 1, the NH of 1.0g / L 4 Cl, 0.3g / L of K 2 HPO 4 , 0.3g / L of KH 2 PO 4 , 0.1g / L KCl, 0.1g / L CaCl 2 2H 2 The NaCl of 0,30g / L, the yeast extract of 0.1g / L, the cysteine ​​of 0.5g / L, the sodium thiosulfate of 3.16g / L and 1ml trace element nutrient solution are mixed to obtain the culture medium solution of 1L, Wherein the trace element nutrient solution contains copper, manganese, zinc, iron, magnesium, molybdenum and boron, and the culture medium solution is adjusted to be 7 with the KOH solution of 10M, and the culture medium solution after adjusting the pH according to the mass grams of agar powder In terms of the percentage of volume in milliliters, add 0.5% agar powder, heat and dissolve to prepare a solution;

[0069] Step 2, uniformly disperse polyvinyl alcohol fibers with a length of 5 mm in 5% NaOH solution, stir at 60° C. for 8 hours, collect the fibers and dry them;

[0070] Step 3, according to the ratio of the quality of the modified polyvinyl alcohol...

Embodiment 3

[0080] Step 1, the NH of 1.0g / L 4 Cl, 0.3g / L of K 2 HPO 4 , 0.3g / L of KH 2 PO 4 , 0.1g / L KCl, 0.1g / L CaCl 2 2H 2 The NaCl of 0,30g / L, the yeast extract of 0.1g / L, the cysteine ​​of 0.5g / L, the sodium thiosulfate of 3.16g / L and 1ml trace element nutrient solution are mixed to obtain the culture medium solution of 1L, Wherein the trace element nutrient solution contains copper, manganese, zinc, iron, magnesium, molybdenum and boron, and the KOH solution of 10M is used to adjust the culture medium solution to a pH of 7.3, and the culture medium solution after adjusting the pH according to the mass grams of agar powder In terms of the percentage of volume in milliliters, add 0.5% agar powder, heat and dissolve to prepare a solution;

[0081] Step 2, uniformly dispersing polyvinyl alcohol fibers with a length of 8mm in 5% NaOH solution, stirring at 55°C for 10h, collecting the fibers and drying them;

[0082] Step 3, according to the ratio of the quality of the modified poly...

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Abstract

The present invention relates to a method and a sampling device for separating and purifying water body micro-organism strains. The method comprises the following steps: step 1, a sample to be separated and purified and a calcium carbonate solution are added to a sodium alginate solution to obtain a precipitate and a suspension; step 2, the suspension is added to an oil phase A composed of sorbitan fatty acid ester and liquid paraffin to obtain a mixed system A; step 3, 6-10 ml of glacial acetic acid is added to the mixed system A, reaction is conducted at 22-25 DEG C for 8-12 min to obtain amixed system B, a calcium chloride solution is added to the mixed system B to obtain microcapsules, and the microcapsules are washed with sterilized water to obtain a microcapsule micro-organism strain bank; and step 4, the microcapsule micro-organism strain bank is inoculated one by one into porous plates to be cultured to obtain single colonies, and the porous plates are loaded with a BM liquidculture medium solution loaded with the micro-organism strains to be separated and purified. The method combines an emulsified micro-balloon method and a porous plate method, and can be used for database foundation and separation for environment functional micro-organism ecological germplasm resources.

Description

technical field [0001] The invention relates to the field of separation and cultivation of water body microorganisms, in particular to a method for separation and purification of water body microorganism strains and a sampling device. Background technique [0002] The purification of microbial strains refers to the process of separating different types of microorganisms mixed together to obtain a single strain required for production and research. It is a key technology for the application and research of microbial industries and is widely used in microbial anti-corrosion, Industrial fields and experimental research fields such as water pollution control, mineral smelting, and functional bacterial agent development. In the specific purification, the corresponding culture medium is generally prepared according to the strains to be purified for separate isolation. In the purification of microbial strains, the existing methods mainly include ten-fold dilution separation method...

Claims

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Application Information

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IPC IPC(8): C12N1/02C12M1/26
CPCC12M33/04C12N1/02
Inventor 朱超杨永林王慧琴马宏瑞贾柳陈喆倩
Owner SHAANXI UNIV OF SCI & TECH
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