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A kind of preparation method of single particle double emission ratio fluorescent probe and its application

A ratiometric fluorescent probe, dual emission technology, applied in the fields of chemistry and nanomaterials science, can solve the problems of expensive, cumbersome and time-consuming equipment, cumbersome processing, avoid instability problems, simple operation, good selectivity and sensitivity. sexual effect

Active Publication Date: 2022-08-09
中科合肥智慧农业谷有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, common detection methods mainly include high performance liquid chromatography, gas chromatography-mass spectrometry, etc., but these methods have certain disadvantages, such as cumbersome sample pretreatment process, expensive and cumbersome equipment, and long time-consuming, so simple and fast detection methods are urgently needed

Method used

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  • A kind of preparation method of single particle double emission ratio fluorescent probe and its application
  • A kind of preparation method of single particle double emission ratio fluorescent probe and its application
  • A kind of preparation method of single particle double emission ratio fluorescent probe and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Preparation of aminated blue carbon dots

[0044]Dissolve 0.6g polyethyleneimine and 1g citric acid in 30mL ultrapure water, transfer it to a 50mL reaction kettle, react at 200°C for 6h, cool to room temperature, dialyze it with a dialysis bag with a molecular weight cut-off of 1KDa for 48h, and store it later Refrigerator at 4°C.

[0045] 2. Preparation of red cadmium telluride quantum dots

[0046] Mix 0.0638g tellurium powder and 0.10g NaBH 4 Mixed with 4 mL of ultrapure water, continuously introduced nitrogen to maintain an oxygen-free environment; stirred the reaction in an ice bath for 8 to 10 h, the reaction solution gradually changed from black to pink to white, and NaHTe was formed in the upper layer solution; 0.2284 g of chlorinated Cadmium and 210μL of mercaptopropionic acid were dissolved in 100mL of ultrapure water, the pH of the solution was adjusted to 9 with 1M NaOH, and then the oxygen in the solution was removed by bubbling with nitrogen; 0.5-1M d...

Embodiment 2

[0057] 1. Preparation of aminated blue carbon dots

[0058] The preparation process of this step is the same as that in Example 1.

[0059] 2. Preparation of red cadmium telluride quantum dots

[0060] The preparation process of this step is the same as that in Example 1.

[0061] 3. Preparation of carboxylated red CdTe quantum dot-embedded silica nanoparticles

[0062] The preparation process of this step is the same as that in Example 1.

[0063] 4. Construction of ratiometric fluorescent probes

[0064] The preparation process of this step is the same as that in Example 1.

[0065] 5. Preparation of Gold Nanoparticle Quenching Ratio Fluorescent Probes

[0066] The preparation process of this step is the same as that in Example 1.

[0067] 6. The mixed system of fluorescence quenching is used for the detection of Fumei Shuang

[0068] To the mixed system of gold nanoparticles and ratio probes, add the solution of fumetidine with concentrations of 0.04, 0.1, 0.4, 0.8, ...

Embodiment 3

[0072] 1. Preparation of aminated blue carbon dots

[0073] The preparation process of this step is the same as that in Example 1.

[0074] 2. Preparation of red cadmium telluride quantum dots

[0075] The preparation process of this step is the same as that in Example 1.

[0076] 3. Preparation of carboxylated red CdTe quantum dot-embedded silica nanoparticles

[0077] The preparation process of this step is the same as that in Example 1.

[0078] 4. Construction of ratiometric fluorescent probes

[0079] The preparation process of this step is the same as that in Example 1.

[0080] 5. Preparation of Gold Nanoparticle Quenching Ratio Fluorescent Probes

[0081] The preparation process of this step is the same as that in Example 1.

[0082] 6. The mixed system of fluorescence quenching is used for the detection of Fumei Shuang

[0083] To the mixed system of gold nanoparticles and ratio probes, add the solution of fumetidine with concentrations of 0.04, 0.1, 0.4, 0.8, ...

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Abstract

The invention discloses a preparation method and application of a single-particle dual-emission ratio fluorescent probe, wherein the preparation method of the single-particle dual-emission ratio fluorescent probe is to first prepare silicon oxide for carboxylation-embedded red CdTe quantum dots by a method, Aminated blue carbon dots were then covalently coupled to the surface to construct a dual-emission ratiometric fluorescent probe. The fluorescence quenching system constructed by the ratio fluorescent probe combined with gold nanoparticles of the present invention can be used for fluorescence-enhanced detection of pesticide fumetidine. Based on the fluorescence resonance energy transfer between gold nanoparticles and carbon dots, blue fluorescence is quenched, and red fluorescence is quenched. Silicon oxide is used as the internal standard, and after adding Fomeshuang, the strong bonding between gold nanoparticles and sulfur atoms leads to their agglomeration, the blue fluorescence recovers, and the process of turning off and then turning on the blue fluorescence is realized, so as to realize the detection of Fomeshuang. The method has high sensitivity, good selectivity, strong anti-interference ability and low detection limit, and can also be used for the detection of actual samples.

Description

technical field [0001] The invention relates to a preparation method and application of a single-particle double-emission ratio fluorescent probe, belonging to the field of chemistry and nanomaterial science. Background technique [0002] As a typical fungicide and preservative, Pesticide Fumei Shuang is widely used in fruits, vegetables and crops to prevent diseases and insect pests of various crops, thereby improving their yield and quality. However, the abuse of Fumei Shuang will lead to serious pesticide residue problems, resulting in the pollution of drinking water and groundwater, making humans suffer from diseases on the skin and mucous membranes, and seriously affecting human body and physical and mental health. Therefore, it is of great significance to rapidly and quantitatively detect Famex. [0003] At present, the common detection methods mainly include high performance liquid chromatography, gas chromatography-mass spectrometry, etc., but these methods have cer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02C09K11/88B82Y20/00B82Y30/00G01N21/64
CPCC09K11/025C09K11/883B82Y20/00B82Y30/00G01N21/643
Inventor 蒋长龙杨亮王振洋张淑东刘变化
Owner 中科合肥智慧农业谷有限责任公司