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Sainfoin anther callus differentiation culture medium

A technology of differentiation medium and callus, which is applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., and can solve the problems of low rate of differentiation into seedlings, low frequency of callus induction, difficulties in haploid plants, etc. Problems, to achieve the effect of increasing the rate of acquisition, great practical significance and practical value, and increasing the rate of differentiation into seedlings

Inactive Publication Date: 2019-09-10
郭根霞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are not many domestic studies on the culture of red bean grass anthers. Although there are reports of obtaining haploid plants, the problems of low callus induction frequency and low rate of differentiation into seedlings produced by anther culture have not been well resolved. , it is still very difficult to obtain haploid plants through anther culture, and it is impossible to apply it to breeding practice

Method used

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  • Sainfoin anther callus differentiation culture medium
  • Sainfoin anther callus differentiation culture medium
  • Sainfoin anther callus differentiation culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take the anther callus of common red bean grass and Caucasian red bean grass as test materials. When the callus grows to 3-4mm in size, transfer it to the differentiation medium and place it at a temperature of 24°C, a light intensity of 2000 lx, and a light time of 14 hours. Cultivate for 30 days under the condition of / d, and count the differentiation rate of green seedlings. Green seedling differentiation rate = (the number of calli that differentiated into adventitious buds or seedlings / the total number of inoculated calli) × 100%.

[0017] The composition and concentration of the differentiation medium are: KNO 3 2825mg / L, (NH 4 ) 2 SO 4 340mg, Ca (NO 3 ) 2 208mg / L, MgSO 4 ·7H 2 O 179 mg, KH 2 PO 4 206mg / L, K 2 HPO 4 169mg / L, KI 0.81mg / L, H 3 BO 3 4.6mg / L, MnSO 4 4H 2 O 21.5mg / L, ZnSO 4 ·7H 2 O 8.75mg / L, Na 2 MoO 4 2H 2 O 0.23mg / L, CuSO 4 ·5H 2 O2.5mg / L, CoCl 2 ·6H 2 O 0.025mg / L, iron glycinate 29.4mg / L, inositol 100mg / L, niacin 1.3mg / L,...

Embodiment 2

[0018] Embodiment 2: the effect of titanium citrate on the callus differentiation of red bean herb anthers

[0019] Preparation of other components is the same as the differentiation medium in Example 1, and the concentration of titanium citrate is respectively 0, 0.5, 1.0, 1.5, 2.0, 2.5, 7 kinds of differentiation medium of 3.0 mg / L, according to the method in Example 1 The anther calluses of Ormosia sativa and Caucasian sargassum were respectively transferred to these differentiation mediums, and the differentiation rate of green shoots was counted. The results are shown in Table 1.

[0020]

[0021] Titanium citrate is a plant growth regulator. After the plant absorbs it, the chlorophyll content in the body increases, photosynthesis is enhanced, the content of catalase is increased, the activity of nitrate reductase is enhanced, and it can also accelerate the growth of plant roots and promote the growth of plants to the soil Absorption of macronutrients and micronutrient...

Embodiment 3

[0022] Embodiment 3: the influence of sodium nitroprusside on callus differentiation of red bean herb anthers

[0023] Preparation of other components is the same as the differentiation medium in Example 1, and the concentration of sodium nitroprusside is respectively 6 kinds of differentiation medium of 0, 10, 20, 30, 40, 50 μmol / L, and the common red bean is made according to the method in Example 1 The anther calluses of Grass and Caucasian Ormosia were transferred to these differentiation media respectively, and the differentiation rate of green shoots was counted. The results are shown in Table 2.

[0024]

[0025] Sodium nitroprusside is a commonly used nitric oxide donor. As an important signaling molecule, nitric oxide participates in the regulation of various physiological processes in plant tissue cells, and has a wide range of effects on plant growth and development. It can be seen from Table 2 that when 10-40 μmol / L sodium nitroprusside was added to the differen...

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Abstract

The invention discloses a sainfoin anther callus differentiation culture medium, which comprises KNO3, (NH4)2SO4, Ca(NO3)2, MgSO4*7H2O, KH2PO4, K2HPO4, KI, H3BO3 and other trace elements, iron glycinate, inositol, nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride, valine, taurine, glutamine, tea polyphenol, ZT, diethylaminoethyl hexanoate, titanium citrate, lanthanum nitrate, polyvinylpyrrolidone, sodium nitroprusside, colchicine, maltose, plant gel and mulberry leaf juice. According to the sainfoin anther callus differentiation culture medium, the green seedling differentiationrate of the sainfoin anther callus is greatly improved, and thus the acquisition rate of regenerated plants from flower culture is improved, and a foundation is laid for the practical application ofthe sainfoin haploid breeding.

Description

technical field [0001] The invention relates to a callus differentiation medium of red bean herb anthers, in particular to a differentiation medium for improving the rate of differentiation of red bean grass anther callus into seedlings, and belongs to the field of forage plant biotechnology. Background technique [0002] Legume grass refers to the forage group composed of legume forage plants, also known as legume grass. Leguminous herbage has high protein content, good palatability, symbiosis with rhizobia, can fix nitrogen in the atmosphere to provide nitrogen nutrition for itself, and improve soil fertility. It is often used as green manure and nectar source plants. There are many kinds of leguminous forages, mainly including alfalfa, red bean grass, clover, sweet clover, milk vetch, lotus root, etc. Among them, red bean grass has high nutritional value and rich crude protein content, and is known as the "grass queen". Red bean grass is a perennial herbaceous plant belo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 郭根霞
Owner 郭根霞
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