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Chaetoceros muelleri culture medium composition and culture method, and photobioreactor

A technology of photobioreactor and Chaetoceros mouvii, which is applied in the direction of photobioreactor, specific-purpose bioreactor/fermenter, combination of bioreactor/fermenter, etc. Stability, the inability to achieve stable high-quality and high-yield, low utilization rate of carbon dioxide, etc., to achieve the effect suitable for large-scale production, small error, and small workload

Pending Publication Date: 2019-09-20
GUANGXI ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Outdoor lighting has low light rate, large loss of light energy, and insufficient supply at night
The use of artificial light sources has the disadvantage of consuming a large amount of electric energy, which is not conducive to reducing the cost of large-scale cultivation of microalgae
[0007] 2. The open culture mode is easy to cause microalgae to be polluted by other miscellaneous algae and pathogens, which affects the purity, yield and stability of the culture, and cannot achieve stable, high-quality and high-yield
[0008] 3. The utilization rate of carbon dioxide is not high
At present, a pipeline system with an outlet is mostly used to pass air into the microalgae suspension, but when this method is used to cultivate CO 2 In the culture medium, the solubility is low, the bubble diameter is large, and the residence time is short, which is not conducive to the absorption of algae

Method used

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  • Chaetoceros muelleri culture medium composition and culture method, and photobioreactor
  • Chaetoceros muelleri culture medium composition and culture method, and photobioreactor
  • Chaetoceros muelleri culture medium composition and culture method, and photobioreactor

Examples

Experimental program
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Effect test

Embodiment 1

[0052] A culture medium composition and an efficient culture method of Chaetoceros mouchii, the preparation method of the culture medium composition is as follows:

[0053] (a1) Mother liquor A: Put sodium nitrate, potassium dihydrogen phosphate, and sodium silicate into double-distilled water in turn. When putting them in, put in the next raw material after the previous raw material is completely dissolved, and sterilize to obtain the mother liquor A;

[0054] (a2) Mother liquor B: put ferric citrate and sodium molybdate into double distilled water, heat and stir until boiling, filter the solution with filter paper after cooling, and sterilize to obtain mother liquor B;

[0055] (a3) Mother liquor C: Put manganese chloride, copper sulfate, zinc sulfate, cobalt chloride and EDTA-disodium into double-distilled water, completely dissolve and sterilize to obtain mother liquor C;

[0056] (a4) Mother solution D: Sterilize with double distilled water, add VB after cooling 1 and V...

Embodiment 2

[0066]A culture medium composition and efficient culture method of Chaetoceros mouchii, the difference between the preparation method of the culture medium composition and Example 1 is that the dark precipitation time of the seawater in step (a5) is 5.5 days; the The components and concentrations of the medium composition are as follows:

[0067] Sodium nitrate 78mg / L, potassium dihydrogen phosphate 6mg / L, sodium silicate 39mg / L, ferric citrate 4.2mg / L, manganese chloride 1.6×10 -1 mg / L, copper sulfate 0.7×10 -3 mg / L, zinc sulfate 2.3×10 -2 mg / L, sodium molybdate 7.1×10 -3 mg / L, cobalt chloride 1.2×10 -2 mg / L, EDTA-disodium 4.3×10 -3 mg / L, VB 1 0.07mg / L, VB 12 4.6×10 -4 mg / L;

[0068] Described cultivation method comprises the steps:

[0069] (b1) In a photobioreactor with a volume of 500L, add a potassium permanganate solution with a concentration of 9 ppm and soak for 6 minutes, then rinse it off with sterilized seawater. The disinfection method of the sterilized se...

Embodiment 3

[0073] A culture medium composition and efficient culture method of Chaetoceros mouchii, the difference between the preparation method of the culture medium composition and Example 1 is that the dark precipitation time of the seawater in step (a5) is 6 days; The components and concentrations of the medium composition are as follows:

[0074] Sodium nitrate 82mg / L, potassium dihydrogen phosphate 7mg / L, sodium silicate 42mg / L, ferric citrate 4.5mg / L, manganese chloride 1.7×10 -1 mg / L, copper sulfate 0.9×10 -3 mg / L, zinc sulfate 2.6×10 -2 mg / L, sodium molybdate 7.2×10 -3 mg / L, cobalt chloride 1.4×10 -2 mg / L, EDTA-disodium 4.6×10 -3 mg / L, VB 1 0.10mg / L, VB 12 4.8×10 -4 mg / L;

[0075] Described cultivation method comprises the steps:

[0076] (b1) In a photobioreactor with a volume of 500L, add a potassium permanganate solution with a concentration of 10ppm and soak for 3 minutes, then rinse it with sterilized seawater. The disinfection method of the sterilized seawater is...

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Abstract

The invention discloses a chaetoceros muelleri culture medium composition and a high-efficiency culture method, wherein the culture medium composition is composed of sodium nitrate, potassium dihydrogen phosphate, sodium silicate, ferric citrate, manganese chloride, copper sulfate, zinc sulfate, sodium molybdate, cobalt chloride, EDTA-disodium, VB1, VB12 and the like. The culture method comprises the steps: putting the culture medium composition into a disinfected closed photobioreactor, fully aerating for 10-15 min, then adding a first-order algae seed solution with the inoculation density of 0.5-1.4*10<6> cell / mL, controlling the temperature of the material in the photobioreactor to be 25-34 DEG C, the salinity to be 0.5-4%, the pH to be 7.7-8.3 and the illumination intensity to be 3500-7000 Lx, determining to introduce air and carbon dioxide into the material respectively according to the change of the pH value in water bodies, and culturing for 3-5 d for harvesting.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a culture medium composition, a culture method and a photobioreactor of Chaetoceros mouroii. Background technique [0002] As a renewable biomass energy, microalgae has also received more and more attention. There are also related researches on the industrialization of large-scale cultivation of microalgae and some progress has been made. However, due to the large-scale cultivation of microalgae, it is easy to Due to the influence of environmental conditions and other factors, the existing microalgae industrialization model still has shortcomings such as low efficiency, high cost and unstable output. [0003] Chaetoceros muelleri belongs to Bacillariophyta, Centricae, Biddulphiales, Chaetoceraceae, Chaetoceros. It is a small marine planktonic diatom with small cells and thin cell walls. Most of them live alone, and there are also 2-3 cells connected to form groups. Chaetoce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12M1/38C12M1/34C12M1/04C12M1/00C12R1/89
CPCC12N1/12C12M21/02C12M23/22C12M23/24C12M23/38C12M23/48C12M29/06C12M31/10C12M39/00C12M41/26C12M41/32C12M41/12
Inventor 张彬朱昔恩熊建华谢达祥赵永贞陈晓汉韦嫔媛陈田聪彭金霞何苹萍
Owner GUANGXI ACADEMY OF FISHERY SCI
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