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A kind of priming agent for biogas residue aerobic fermentation and preparation method thereof

An aerobic fermentation and priming agent technology, applied in the field of agricultural waste recycling, can solve the problems of slow heating rate of biogas residues, poor quality of aerobic fermentation products, unfavorable large-scale treatment of biogas residues aerobic fermentation, etc. Effects of aerobic fermentation efficiency of slag, improvement of aerobic fermentation product quality, efficiency and harmless level

Active Publication Date: 2021-09-21
ACADEMY OF PLANNING & DESIGNING OF THE MINIST OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Biogas residue is rich in nutrients and is an excellent raw material for organic fertilizer. However, biogas residue is mainly composed of cellulose and other refractory organic matter, with low C / N ratio, high moisture content, and small porosity, which lead to aerobic fermentation of biogas residue. The heating rate of the process is slow, the high temperature period is short, it is not conducive to decomposing, and the quality of aerobic fermentation products is poor, etc.
Patent CN201810265985.9 provides a rapid aerobic fermentation method for biogas residues, but in this method, the aerobic fermentation process has large ventilation and high energy consumption, which is not conducive to the large-scale treatment of biogas residues aerobic fermentation
Studies have shown that in the aerobic fermentation process with biogas residue as the main raw material, the aerobic fermentation mixture can heat up quickly within 24 hours in summer, but the temperature of the material in winter rises slowly.

Method used

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  • A kind of priming agent for biogas residue aerobic fermentation and preparation method thereof
  • A kind of priming agent for biogas residue aerobic fermentation and preparation method thereof
  • A kind of priming agent for biogas residue aerobic fermentation and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0047] (1) Mix Bacillus, Lactobacillus, Enterococcus, Brevibacillus, Aromatobacter, and Acetobacter according to the ratio of 20:44:15:8:8:5, and add it to the microbial culture medium (into 1L of water Add 6g of beef extract, 15g of peptone, 6g of sodium chloride, and prepare by sterilizing at 121°C for 15 minutes), and incubate at 25°C for 24 hours to obtain a composite microbial agent; the number of viable bacteria per gram of the compound microbial agent can reach 1× 10 9 above;

[0048] (2) Mix biochar, wood acetic acid and microbial culture medium evenly in the ratio of 10g: 15ml: 75ml to obtain a high-efficiency immobilization medium.

[0049] (3) Take 1g of compound microbial agent and place it in a 250ml Erlenmeyer flask, add 8ml of high-efficiency immobilization medium to ensure that each gram contains 1×10 beneficial bacteria 9 Above, adsorb and fix in a 150rpm shaker at 25°C for 24h, centrifuge at 4000r / min for 15min, pour off the supernatant, and freeze-dry the ...

Embodiment 2

[0051] (1) Mix Bacillus, Lactobacillus, Enterococcus, Brevibacillus, Aromatobacter, and Acetobacter according to the ratio of 20:44:13:9:9:5, and add it to the microbial culture medium (into 1L of water Add 6 g of beef extract, 15 g of peptone, 6 g of sodium chloride, and sterilize at 121° C. for 15 minutes to prepare), and incubate at 25° C. for 28 hours to obtain a composite microbial bacterial agent.

[0052] (2) Mix biochar, wood acetic acid and microbial culture medium evenly in the ratio of 8g: 18ml: 72ml to obtain high-efficiency immobilized culture medium.

[0053] (3) Take 1g of compound microbial agent and place it in a 250ml Erlenmeyer flask, add 10ml of high-efficiency immobilization medium to ensure that each gram contains more than 1 billion beneficial bacteria, absorb and fix it in a 150rpm shaker at 25°C for 30h, 4000r / Centrifuge for 20 minutes at 1 min, pour off the supernatant, and freeze-dry the lower layer of sediment to become the primer for biogas residu...

Embodiment 3

[0055] (1) Mix Bacillus, Lactobacillus, Enterococcus, Brevibacillus, and Aroma-like bacteria at a ratio of 20:45:15:10:10 (excluding Acetobacter), and add them to the microbial culture medium (to each Add 6 g of beef extract, 15 g of peptone, and 6 g of sodium chloride into 1 L of water, prepare by sterilizing at 121° C. for 15 minutes, and incubate at 25° C. for 26 hours to obtain a composite microbial agent.

[0056] (2) Mix biochar, wood acetic acid, and microbial culture medium evenly in the ratio of 10g: 20ml: 70ml to obtain a high-efficiency immobilized medium.

[0057] (3) Take 1g of compound microbial agent and place it in a 250ml Erlenmeyer flask, add 10ml of high-efficiency immobilization medium to ensure that each gram contains more than 1 billion beneficial bacteria, absorb and fix it in a 150rpm shaker at 25°C for 30h, 4000r / Centrifuge at 1 min for 15 min, pour off the supernatant, and freeze-dry the lower layer to become the priming agent of the present inventio...

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Abstract

The invention provides a primer for biogas residue aerobic fermentation and a preparation method thereof. The priming agent includes compound microbial bacterial agent, biochar, wood acetic acid and microbial culture medium. The composite microbial bacterial agent is prepared by mixing and culturing different species of microbial bacterial agents; the microbial bacterial agent includes: bacillus, lactic acid bacteria, enterococcus, brevibacillus, flavor-like bacteria and acetic acid bacteria. The present invention aims at key technical bottlenecks such as slow heating rate, short high temperature period, and unfavorable decomposing process in the biogas residue aerobic fermentation process, and combines the principles of physics, chemistry and microbiology for the change of organic matter and microbial community structure in the biogas residue aerobic fermentation process , research and develop environmentally friendly, high-efficiency and low-cost biogas residue aerobic fermentation initiators to shorten the heating time of biogas residue aerobic fermentation, prolong the high temperature period, improve the quality of aerobic fermentation products, improve the efficiency of biogas residue aerobic fermentation, and contribute to the treatment and resources of biogas residues Provide technical support for chemical utilization.

Description

technical field [0001] The invention belongs to the technical field of resource utilization of agricultural waste, and in particular relates to a primer for aerobic fermentation with biogas residue as a main raw material and a preparation method thereof. Background technique [0002] Aerobic fermentation is an effective way of resource utilization of organic waste. Its essence is to make organic matter mineralized, humified and harmless through high-temperature fermentation under the action of microorganisms under certain conditions of moisture, C / N ratio and ventilation. The process of becoming decomposed fertilizer. The characteristics of fermentation raw materials, C / N ratio, moisture, temperature, ventilation conditions, pH value, etc. are closely related to the growth and activity of aerobic fermentation microorganisms, which in turn affect the efficiency of aerobic fermentation and the maturity of fermented products. A complete aerobic fermentation process can be divi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F11/08
CPCC05D9/00C05F11/08C05F11/00
Inventor 沈玉君孟海波隋斌张曦程红胜宋立秋郑圣尉
Owner ACADEMY OF PLANNING & DESIGNING OF THE MINIST OF AGRI
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