A kind of 3D printing flexible porous support material and preparation method thereof

Abstract

The invention provides a method for preparing a 3D printing flexible porous support material, comprising the following steps: a) preparing 3D printing ink; the solute of the 3D printing ink is one or more of PLA, PLGA, PCL and PC; The solvent of the 3D printing ink is one or more of NMP, DMF and DMSO; b) under the power of an air source, use the 3D printing ink obtained in step a) to perform 3D printing on the receiving device to obtain a fiber support; c) performing solvent replacement on the fibrous scaffold obtained in step b), and freeze-drying to obtain a 3D printed flexible porous scaffold material. The preparation method has a wide selection of materials, a simple preparation process, and can be carried out at low temperature; and the surface of the prepared fiber has a micro-nano pore structure, which is conducive to the loading of active substances such as growth factors and drugs, and the rough The fiber surface is conducive to cell adhesion and proliferation.

Description

technical field [0001] The invention relates to the technical field of regenerative medicine, and more specifically, relates to a 3D printing flexible porous scaffold material and a preparation method thereof. Background technique [0002] The structural and morphological characteristics of materials determine biological processes such as cell adhesion, proliferation, and differentiation. The macrostructure regulates the adhesion and proliferation of cells, and the microstructure can affect the biological behavior of cells through the release characteristics of the growth factors and drugs loaded and the exchange process of nutrients in the surrounding cells. Therefore, an ideal tissue engineering scaffold should fully simulate the mechanical properties and biological properties of the extracellular matrix, so that the prepared scaffold has good biocompatibility and biodegradability, and can realize the precise structure of multi-level structure or gradient structure. contr...

AI Technical Summary

Problems solved by technology

FDM printing technology uses polymer fibers with a diameter of 1.75-3mm. The polymer material is melted and extruded at the print head, and then forms a device structure on the receiving platform; this requires the polymer to have good processability, and it The melt state and solidification state of the polymer can change rapidly. If the heating time is too long or the temperature is too high during the melting process, it will easily lead to the degradation of the polymer, and the viscosity of the polymer is too high when it is melted, which also brings difficulties to processing, and Under high temperature conditions, active substances such as growth factors are easily inactivated; these shortcomings limit the application of this technology in the field of tissue engineering

SLA printing technology uses light-curing molding technology, which can quickly solidify and form, and the printing accuracy is very high, but in this method, the selection of materials is very limited, and a series of light-curing initiators need to be added to the printing materials. Body and other components, such printed devices will cause residual monomers and initiators and other substances, and it is difficult to remove, which will cause great risks when implanted in the body.

SLS technology uses laser sintering method for 3D printing, and the printing process temperature is very high. This method is suitable for 3D printing of metal materials, and is not suitable for loading growth factors and drugs required for tissue engineering scaffolds.

At the same time, the surface of the device prepared by the above methods all presents a solid smooth structure without surface pores of micro-nano structure

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