Application of Galantamine in Prevention and Treatment of Fish Enteritis Caused by Soybean Meal Feed

A technology of galantamine and fish, applied in the application field of fish enteritis, to achieve the effect of maintaining tight junctions, reducing costs, and inhibiting intestinal inflammation

Active Publication Date: 2022-07-08
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding its anti-inflammatory effect, it has only been seen to have a certain effect on hyperemia.

Method used

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  • Application of Galantamine in Prevention and Treatment of Fish Enteritis Caused by Soybean Meal Feed
  • Application of Galantamine in Prevention and Treatment of Fish Enteritis Caused by Soybean Meal Feed
  • Application of Galantamine in Prevention and Treatment of Fish Enteritis Caused by Soybean Meal Feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Prediction of the efficacy of Galantamine, a functional ingredient for relieving enteritis

[0038] The KEGG pathway analysis of grass carp transcriptome suggests the existence of fish cholinergic anti-inflammatory pathway: the inventor's previous studies have shown that the intestinal inflammation of grass carp in the 40% soybean meal replacement fish meal group has self-recovery from the 5th week to the 7th week. ability. At each stage of the disease course, including 0 days, 3 weeks, 5 weeks, and 7 weeks, the post-intestinal tissue was taken to measure the transcriptome. The analysis of the KEGG pathway showed that the Acetylcholine pathway was involved in the occurrence and resolution of soybean meal-induced enteritis. are activated ( figure 2 ), suggesting that fish cholinergic anti-inflammatory pathways may exist.

[0039] Then, we used the CMap database to predict the efficacy of the cholinesterase inhibitor galantamine: as a cholinesterase inhibitor...

Embodiment 2

[0046] Example 2 Application of galantamine as feed additive in alleviating soybean meal-induced zebrafish enteritis

[0047] 1. Zebrafish SBMIE modeling and galantamine addition experimental feed formula and preparation method

[0048] Table 3 Zebrafish SBMIE modeling and galantamine supplemented experimental diet formula

[0049]

[0050] FM is the control fish meal feed, 50SBM is the soybean meal feed (50% alternative protein source) used in this example, 50SBM+0.5ppm Gal is the soybean meal feed supplemented with 0.5ppm galantamine, 50SBM+1ppm Gal is the soybean meal feed supplemented with 1ppm galantamine sensitive soybean meal feed.

[0051] The preparation method is as follows:

[0052] (1) Sieve fish meal, soybean meal, starch, flour, cellulose, mineral premix, vitamin premix, etc. through a 60-mesh sieve. Among them, there may be larger particles such as fish bones and beans in the fish meal, soybean meal, and mineral mixture. Skins, etc., should be crushed and ...

Embodiment 3

[0071] Example 3 Intramuscular injection of galantamine reduces the expression of intestinal inflammatory factors after soybean meal-induced enteritis and diseased grass carp

[0072] 1. The grass carp (grass carp SBMIE modeling method and feed formula, etc., refer to Integrative Transcriptomic Study Reveals Immune Mechanism for the Resilience to Soybean Meal Stress in Fish Gut and Liver, Frontier in Physiology) fed with 70% soybean meal feed group and fish meal group for 7 weeks. 10 tails, keep the temperature in the cage at 23-25℃, the dissolved oxygen is more than 5mg / L, and fasting;

[0073] 2. Intramuscular injection of galantamine hydrobromide (0.05-0.1 mg / kg fish body weight) on the back, 2 times a day for a total of 2 days, and intestinal tissue samples were taken on the 4th day;

[0074] 3. Fluorescence quantitative qPCR analysis was performed to detect the expression of intestinal inflammation-related immune genes. Statistical analysis of fluorescence quantitative d...

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Abstract

The invention discloses the application of galantamine in the prevention and treatment of soybean meal-induced fish enteritis. According to the neural regulation theory of intestinal mucosal immunity, combined with two methods of disease modeling and biological information analysis, it is speculated that galantamine is a potential prevention and treatment method. Soybean meal-induced enteritis drug; experiments have shown that galantamine can activate the expression of anti-inflammatory factors in the intestinal tract of farmed fish, inhibit intestinal inflammation, and maintain tight junctions of the intestinal epithelial barrier, whether through feed addition or back intramuscular injection , inhibiting the apoptosis of intestinal epithelium, thereby relieving enteritis caused by soybean meal feed, and fish can recover growth, so galantamine can be used as a new type of feed additive or injection with anti-inflammatory effect.

Description

technical field [0001] The invention belongs to the field of veterinary pharmacy, and specifically relates to the application of galantamine in the prevention and treatment of soybean meal-induced fish enteritis. Background technique [0002] Aquatic animal enteritis is one of the main diseases that cause economic losses in aquaculture. Aquatic animals, especially fish and shrimp, need to add animal protein such as fishmeal to their feed. However, the shortage of fishmeal resources has become a key factor restricting the sustainable development of my country's aquaculture industry. Therefore, the use of vegetable protein sources to replace fishmeal and improve its Utilization rate is an inevitable choice for the development of aquaculture. However, plant protein sources, such as soybean meal, cotton meal, etc., will generally induce intestinal inflammation, liver disease and growth decline in fish when the proportion of replacing fish meal is too large, which limits the appl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/55A61P29/00A61P1/00
CPCA23K50/80A23K10/37A23K20/132A61K31/55A61P29/00A61P1/00Y02P60/87
Inventor 张永安吴南王标
Owner INST OF AQUATIC LIFE ACAD SINICA
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