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Preparation, application method and screening method of Pseudomonas Monterey YS-3

A Monterey pseudo, YS-3 technology, applied in the related application field of Monterey Pseudomonas YS-3, can solve problems such as influence, and achieve the effect of good removal effect

Inactive Publication Date: 2019-12-03
SHENZHEN CHANGLONG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biological denitrification is mainly based on denitrifying bacteria. Under facultative anaerobic conditions, nitrate and nitrite are used as electron acceptors to generate nitrogen. However, in high latitude areas, low temperature has a great impact on biological denitrification technology. Impact

Method used

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  • Preparation, application method and screening method of Pseudomonas Monterey YS-3
  • Preparation, application method and screening method of Pseudomonas Monterey YS-3
  • Preparation, application method and screening method of Pseudomonas Monterey YS-3

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Screening to obtain Pseudomonas monteilii (Pseudomonas monteilii) YS-3, the screening process is as follows:

[0050] Step 1), take activated sludge from the A / A / O treatment process A pool outlet of a municipal sewage plant in Harbin;

[0051] Step 2), inoculating 5g of activated sludge into an inorganic salt medium with a volume fraction of 0.5% nitrate nitrogen, and cultivating it on a shaking table with a temperature of 8°C and a rotating speed of 150 rpm for 3 days to obtain the first bacterium liquid;

[0052] Step 3), absorbing 5% of the first bacterial solution in step 2) and transferring it to an inorganic salt medium with a volume fraction of nitrate nitrogen of 1.5%, cultivating on a shaker at a temperature of 8°C and a rotation speed of 150 rpm for 3 day, get the second bacterial solution;

[0053] Step 4), absorbing 5% of the second bacterial solution in step 3) and transferring it to an inorganic salt medium with a volume fraction of nitrate nitrogen of 2...

Embodiment 2

[0057] The strains screened in Example 1 were identified.

[0058] In Example 1 of the present invention, three strains were screened, which were named HW-1, YS-2, and YS-3 in the present invention. Among them, YS-3 was identified as Pseudomonas monteilii.

[0059] Pseudomonas monteilii (Pseudomonas monteilii) YS-3 grows well on agar plate medium, such as figure 1 As shown, the colonies are regular in shape, white and flat, uniform in size, 2 to 4 mm in diameter, wrinkled on the surface, smooth on the edge, Gram staining is negative, motile, and grow aerobically. The growth temperature of the strain is 10-20°C, pH 7-9, and the optimum growth conditions: the growth temperature is 30°C, pH 8.

[0060] DNA was extracted from the single colony obtained in step 5) in Example 1, and the strain was subjected to DNA sequencing, and its 16S rRNA gene sequence was shown in SEQ ID No.1. In the present invention, the bacterium is also called Pseudomonas monteilii (Pseudomonas monteilii...

Embodiment 3

[0062] The nitrogen compound degradation ability of Pseudomonas monteilii YS-3 bacteria obtained in Example 1 was determined. Specific steps are as follows:

[0063] Step 1), get the Pseudomonas monteilii (Pseudomonasmonteilii) YS-3 obtained in Example 1 and the bacterium liquid of HW-1 and YS-2 obtained by screening at the same time, centrifuge at 4000 rpm for 10 minutes, discard Clear, add actual water to the precipitate and resuspend to obtain Pseudomonas monteilii (Pseudomonas monteilii) YS-3, HW-1, YS-2;

[0064] Step 2), respectively take 30mL of Pseudomonas monterei and the HW-1 and YS-2 screened at the same time according to step 1, and then add them to the test bottles with numbers 2, 3, and 4 respectively, and the number 1 is Control group without bacteria. And in each test bottle, adding nitrate nitrogen is 25.83mg / L municipal treatment water and sodium acetate trihydrate (carbon-to-nitrogen ratio is 10) to total volume is 100mL, number 1 Pseudomonas monterei ( T...

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Abstract

The invention relates to the field of sewage treatment, and a preparation of Pseudomonas Monterey YS-3 that is prepared by using Pseudomonas Monterey YS-3 through a nutrition culture medium. The nutrition culture medium is any one of beef extract peptone culture medium, LB nutrition culture medium and inorganic salt culture medium containing a carbon source; the beef extract peptone culture mediumcomprises 3 g / L of beef extract, 10 g / L of peptone and 5g / L of sodium chloride, and the pH of the beef extract peptone culture medium is 7.0-8.0; the LB nutrition culture medium comprises 5.0 g / L ofyeast powder, 10.0 g / L of sodium chloride and 10.0 g / L of peptone, and the pH of the LB nutrition culture medium is 7.2-7.6; and the inorganic salt culture medium comprises 10 g / L of sodium nitrate, 0.08 g / L of sodium molybdate, 0.12 g / L of ferrous sulfate heptahydrate, 0.2 g / L of magnesium sulfate heptahydrate, 0.12 g / L of calcium chloride, 1 g / L of potassium dihydrogen phosphate, and 1g / L of diammonium hydrogen phosphate, and the pH of the inorganic salt culture medium is 7.2-7.6.

Description

technical field [0001] The present invention relates to the field of sewage treatment, and the present invention relates to Pseudomonas monteellii and application thereof, specifically, relates to Pseudomonas monteilii (Pseudomonas monteilii) YS of nitrogen-containing compounds in sewage treatment waste water in the north in winter -3 for its related applications. Background technique [0002] In recent years, with the improvement of people's living standards and the rapid development of the economy, the nitrogen content discharged into the water body is increasing day by day, and the nitrogen pollution in the sewage is also becoming more and more serious. Nitrogen control has also received more and more attention. Among the many methods for denitrification of wastewater, biological denitrification technology is widely used because of its environmental protection and effective characteristics. Biological denitrification is mainly based on denitrifying bacteria. Under facul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C02F101/16C12R1/38
CPCC12N1/20C12N1/02C02F3/34C02F2101/16B01F25/40
Inventor 董红红
Owner SHENZHEN CHANGLONG TECH CO LTD
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