Preparation method and application of milk-borne active peptide

Abstract

The invention discloses a preparation method and application of milk-borne active peptide. Soybean protein and casein are used as raw materials, and by an enzymolysis and microbial fermentation combined method, a milk-borne active peptide product is obtained. The preparation method comprises the following steps of adding distilled water to the soybean protein according to a certain mass proportion, performing soaking pretreatment, adjusting pH of a system, adding acid protease, and performing a reaction; adding the distilled water to the casein according to a certain proportion, adjusting pH of a system, adding trypsin, and performing a reaction; and then mixing soybean protein enzymatic hydrolysate with casein enzymatic hydrolysate, performing inoculation according to the proportion of 5%, performing fermentation, controlling fermentation pH to be about 7.0, fermentation temperature to be 40-50 DEG C and fermentation time to be 100-120 hours, and after the fermentation is completed, performing low-temperature drying so as to obtain the milk-borne active peptide for animals. The milk-borne active peptide has important promoting effects on maturity and development of functions of digestive tracts of the animals. The milk-borne active peptide for the animals disclosed by the invention can notably increase the food consumption, improve the growth properties of livestock and poultry, and enhance immunocompetence and antioxidant ability of organisms.

Description

technical field [0001] The invention belongs to the field of animal feed, and in particular relates to a preparation method and application of a milk-derived active peptide. Background technique [0002] The animal intestine is not only a place for digestion and absorption of nutrients, but also an important immune organ. Maintaining normal intestinal function is crucial to animal growth and development. Under the conditions of nutrient deficiency, infection, stress and pathology, etc., animals can cause damage to intestinal structure and function, translocation of bacteria and endotoxin, and intestinal mucosal barrier dysfunction, etc., which seriously affect animal health and production performance. Production brings significant economic losses. [0003] At present, soybean protein or casein only adopts a single method of fermentation or enzymatic hydrolysis to prepare active peptides. Among them, the small peptides produced by enzymatic hydrolysis have a heavy bitter tas...

AI Technical Summary

Problems solved by technology

Under the conditions of nutrient deficiency, infection, stress and pathology, etc., animals can cause damage to intestinal structure and function, translocation of bacteria and endotoxin, and intestinal mucosal barrier dysfunction, etc., which seriously affect animal health and production performance. produce significant economic losses

[0003] At present, soybean protein or casein only adopts a single method of fermentation or enzymatic hydrolysis to prepare active peptides. Among them, the small peptides produced by enzymatic hydrolysis have a heavy bitter taste and poor palatability; the small peptides produced by microbial fermentation have a low yield and average Large molecular weight, it is difficult to fully exert potential biological functions

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