Construction method of flavonoid fingerprint spectrum in ginkgo leaf extract or preparation thereof
A fingerprint and construction method technology, which is applied in the field of construction of flavonoid fingerprints in ginkgo biloba extract or its preparations, can solve the problems of complicated operation steps and insufficient process simplicity, achieve excellent separation effect, improve work efficiency, Avoid the effects of liquid phase conditions
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Embodiment 1
[0031] This embodiment provides a method for constructing a fingerprint of flavonoids in Shuxuening Injection, a preparation containing Ginkgo biloba extract, which includes the following steps:
[0032] Step a: Taking rutin as a reference substance, dissolving with 95% methanol in volume concentration to obtain a rutin reference substance solution with a concentration of 0.1 mg / mL.
[0033] Step b: Filter 1 mL of Shuxuening injection, a preparation containing Ginkgo biloba extract, with a 0.45 μm microporous filter membrane, and the obtained continuous filtrate is the test solution of the Ginkgo biloba extract injection.
[0034] Step c. Use high performance liquid chromatography to analyze the test solution and reference solution respectively by high performance liquid chromatography, wherein the chromatographic conditions of the high performance liquid chromatography are: the stationary phase is filled with phenylsilane bonded silica gel Chromatographic column, mobile phase A is 0...
Embodiment 2
[0040] This embodiment provides a method for constructing a fingerprint of flavonoids in Shuxuening Injection, a preparation containing Ginkgo biloba extract, as follows:
[0041] Using the method for constructing the fingerprint of Shuxuening injection as described in Example 1, the HPLC spectra of 13 batches of Shuxuening injection were established, such as figure 2 As shown, the fingerprints of 13 batches of samples were synthesized using the "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System" of the National Pharmacopoeia Commission, such as image 3 As shown, the HPLC fingerprint of Shuxuening injection composed of 36 common peaks is generated, and the chromatographic peak No. 15 is rutin. With reference to the control maps generated by 13 batches of samples, the similarity was calculated, and the similarity was all greater than 0.90.
Embodiment 3
[0070] This embodiment provides a method for constructing a fingerprint of flavonoids in Ginkgo biloba extract, which includes the following steps:
[0071] Step a: Taking rutin as a reference substance, dissolving in methanol with a volume concentration of 100% to obtain a rutin reference substance solution with a concentration of 0.08 mg / mL.
[0072] Step b: Dissolve the Ginkgo biloba extract in methanol with a volume concentration of 100% to prepare a solution with a concentration of 3 mg / mL, and filter to obtain the test solution of the Ginkgo biloba extract.
[0073] Step c. Use high performance liquid chromatography to analyze the test solution and reference solution respectively by high performance liquid chromatography, wherein the chromatographic conditions of the high performance liquid chromatography are: the stationary phase is filled with phenylsilane bonded silica gel Chromatographic column, mobile phase A is 0.1% formic acid aqueous solution, mobile phase B is acetonit...
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