A compound bacterial agent for degrading food waste, its application and a method for degrading food waste
A technology of food waste and compound bacteria, which is applied in the field of compound bacteria and food waste degradation, can solve the problems of high preparation cost, complex bacterial components, single weight loss rate, etc.
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Embodiment 1
[0016] Screening and identification of strains suitable for degrading food waste components
[0017] (1) Take 10g of soil samples collected from various places and add them into a sterilized Erlenmeyer flask with small glass beads and 100mL of sterile water, shake at 30°C and 200r / min for 30min, and carry out the bacterial suspension gradient in the ultra-clean bench dilution, the concentration gradient is 10 -4 、10 -5 、10 -6 、10 -7 、10 -8 、10 -9 , inoculate 200 μL of the bacterial suspension of each concentration on the LB plate, seal the plate with a sealing film and invert it, place it in a constant temperature incubator at 37°C for 24 hours, select typical colonies on a plate with a suitable number of colonies, and separate and purify After that, they were inoculated into starch selection medium, oil selection medium, protein selection medium, and cellulose selection medium respectively, and spread evenly with a sterilized coating rod, sealed the culture dish with a s...
Embodiment 2
[0031] Preparation and Compounding of Microbial Agents for Efficient Degradation of Kitchen Waste
[0032] Food waste simulation medium: starch 5g, casein 5g, soybean oil 6.5g, carboxymethyl cellulose 1.0g, water 80g, K 2 HPO 4 0.2g, KCl 0.1g, MgSO 4 0.05g, FeSO 4 ·7H 2 O 0.01 g.
[0033] in
[0034]
[0035] A is the initial content of a certain component, and B is the remaining content of a certain component.
[0036] (1) Streak the frozen Bacillus amyloliquefaciens and Bacillus subtilis glycerol tube plates with plate-activated LB medium, and culture them at 37°C for 12 hours. Pick well-growing colonies into 700 mL of LB fermentation medium in a 2L shake flask, culture at 37°C for 12 hours at 200 r / min. After 24 hours, the fermentation medium was centrifuged at 8000 r / min for 10 minutes to obtain the sludge of Bacillus amyloliquefaciens and Bacillus subtilis.
[0037] (2) Streak the frozen Aspergillus niger glycerin tube with plate-activated PDA medium, incubat...
Embodiment 3
[0043] Optimum Proportion Selection of Mixed Bacteria
[0044] (1) Mix three kinds of bacteria according to a certain ratio, and mix wet bacteria body with straw powder, peat powder and diatomite, wherein the ratio of straw powder, peat powder and diatomite is 50:30:20. Dry the mixture at 40°C to obtain the bacterial agent. Wherein the thallus (calculated as the wet thallus obtained by removing the culture medium after single bacterium culture) accounts for 15% of the weight of the bacterium agent.
[0045] (2) Insert the bacterial agent into the simulated kitchen waste medium with 1% input amount (mass ratio), and cultivate it at 45°C. The structure is shown in Table 3.
[0046] table 3
[0047]
[0048] As can be seen from Table 3, when Bacillus amyloliquefaciens: Bacillus subtilis: Aspergillus niger is compounded at a ratio of 70:10:20, the degradation rate of starch in 24 hours is 88.9%, the degradation rate of protein in 24 hours is 91.2%, and the degradation rate of...
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