Iron-substituted tungsten phosphorus polyacid polydopamine nano mimic enzyme as well as preparation method and application thereof
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A dopamine nano and enzyme-mimicking technology, applied in the field of nanotechnology and catalytic materials, can solve problems such as analysis and inability to other biological substances
Active Publication Date: 2020-06-30
JILIN UNIV
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However, this nano-hybrid material can only be used for the identification and sensing of folic acid overexpressed tumor cells, and cannot be used for the analysis of other b...
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Embodiment 1
[0110] Embodiment 1 Synthesis of iron-substituted tungstophosphopolyacid polydopamine nanozyme of the present invention
[0111] 1) Preparation of precursor Na 8 [HPW 9 o 34 ]
[0112] Weigh 120g of sodium tungstate dihydrate and dissolve it in 150mL of water at 100°C, add 3mL of concentrated phosphoric acid to it under stirring, and then add 22mL of glacial acetic acid dropwise under vigorous stirring. This operation is completed within 30min, and a white precipitate Gradually appear, then filter, stir and wash the precipitate in 250mL 4mol / L NaCl solution, filter again, and dry at high temperature to obtain the desired substance NaCl 8 [HPW 9 o 34 ].
[0113] 2) Preparation of K 10 P 2 W 18 Fe 4 (H 2 O) 2 o 68
[0114]Dissolve 1.5 mmol of ferric chloride or ferric nitrate in 10 mL of water, and add 0.75 mmol of solid Na to the solution 8 [HPW 9 o 34 ], stir, mix and heat the two to homogeneous phase, filter the precipitate while it is hot, add 5g of solid po...
Embodiment 2
[0119] Example 2 Experiment of iron-substituted tungstophosphopolyacid polydopamine nano-mimetic enzyme catalyzing hydrogen peroxide oxidation of its substrate
Embodiment 2A
[0120] Example 2A Experiment of iron-substituted tungstophosphopolyacid polydopamine nano-mimetic enzyme catalyzing hydrogen peroxide oxidation of o-phenylenediamine (OPD)
[0121] (1) Take 800μL, 0.1M, pH4 phosphate buffer solution, add 500μL, 0.9mM o-phenylenediamine (OPD), 500μL, 250mM H 2 o 2 and 200 μL 25 μg / ml iron-substituted tungstophosphopolyacid polydopamine nano-mimetic enzyme, and then mix the above solution evenly; react at room temperature for 10 minutes.
[0122] (2) Measure above-mentioned mixed solution with ultraviolet-visible absorption spectrophotometer and measure ultraviolet absorption spectrum at 350-700nm respectively, experimental result is as follows image 3 (a) shown. Measure the emission spectrum at 450-700nm with a fluorescence spectrophotometer, and the experimental results are as follows image 3 (b) shown.
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Abstract
The invention discloses an iron-substituted tungsten phosphorus polyacid polydopamine nano mimic enzyme as well as a preparation method and application thereof. The invention belongs to the field of nanotechnology and catalytic materials. The structural formula of the iron-substituted tungsten phosphorus polyacid polydopamine nano mimic enzyme is K10P2W18Fe4 (H2O) 2O68/PDA, and the iron-substituted tungsten phosphorus polyacid polydopamine nano mimic enzyme is obtained by preparing a precursor Na8 [HPW9O34], preparing K10P2W18Fe4 (H2O) 2O68 and preparing the iron-substituted tungsten phosphorus polyacid polydopamine nano mimic enzyme. The material disclosed by the invention has a catalytic function of peroxide mimic enzyme and can be used as novel peroxide mimic enzyme; the iron-substituted tungsten phosphorus polyacid polydopamine nano mimic enzyme can replace horse radish peroxidase to be applied to immunoassay, biological detection and clinical diagnosis; in addition, the iron-substituted tungsten phosphate polydopamine nano mimic enzyme prepared by the invention can be used for colorimetric or fluorescent detection of hydrogen peroxide, glutathione and escherichia coli, and thedetection sensitivity is relatively good.
Description
technical field [0001] The invention belongs to the field of nanotechnology and catalytic materials, and in particular relates to an iron-substituted tungstophosphopolyacid polydopamine nano-mimetic enzyme with peroxidase activity, its preparation method, use method and application. The iron-substituted tungstophosphate polydopamine nano-mimetic enzyme is a nano-structure composite material. More specifically, the present invention provides a method for detecting hydrogen peroxide, glutathione and Escherichia coli O157:H7 using iron-substituted tungstophosphate polydopamine nanomimetic enzymes. Background technique [0002] All life phenomena in nature are related to enzymes. Enzymes are biomolecules with catalytic functions, mainly proteins. Natural enzymes have high catalytic efficiency and specific substrates, which play an important role in biochemistry and have been widely studied. As an important natural enzyme, horseradish peroxidase (HRP) can be combined with antib...
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