Calcium butyrate microcapsule as well as preparation method and application thereof

Abstract

The invention provides a calcium butyrate microcapsule as well as a preparation method and application thereof, and relates to the technical field of microcapsules. According to the method, bacteria in a sodium butyrate fermentation process are used as a capsule material, after clostridium butyricum fermentation is finished, the bacteria are subjected to wall breaking, butyric acid is adsorbed into cells again, and volatilization of butyrate odor is avoided through re-embedding of the bacteria on the butyric acid. The preparation process of the calcium butyrate microcapsule is simple, no extrachemical reagent is added, basically no toxic and harmful chemical reagent needs to be introduced, and the risk of solvent residues is avoided; the bacteria are used as a capsule material, so that the taste masking effect is better, and the slow release of a medicine can be realized to a certain extent; and bacterial protein, saccharides, vitamins, nutritional factors and the like in the bacteriacan be used as nutritional ingredients for animals.

Description

technical field

[0001] The invention belongs to the technical field of microcapsules, in particular to a calcium butyrate microcapsule and its preparation method and application. Background technique

[0002] Clostridium butyricum was first discovered and reported by Dr. Miyari Kinji of Chiba Medical University in Japan in 1933. Therefore, it is also known as Miyari bacteria. The classification belongs to the genus Clostridium and is a Gram-positive anaerobic bacillus. In 1935, Dr. Kingimiyairi of the Russian Institute of Microbiology isolated Clostridium butyricum from human feces and soil, and later found that its anaerobic cultured filtrate contained less fatty acids, which had a strong intestine-regulating effect, and it could inhibit The pathogenic bacteria in the intestinal tract can promote the growth of beneficial bacteria in the intestinal tract such as Bifidobacteria and Lactobacillus. In 1992, the Institute of Microbiology of the Heilongjiang Academy of Sciences i...

Examples

preparation example Construction

[0028] The invention provides a preparation method of calcium butyrate microcapsules, comprising the following steps: (1) inoculating Clostridium butyricum into a seed culture medium for cultivation, collecting the thalli in the culture solution, and obtaining the bacteria sludge after cleaning;

[0029] (2) Add NaCl solution and proteinase K to the bacterium slime, stir at 55-60°C for 1-3 hours, and then carry out wall-breaking treatment, collect the bacterium precipitate after breaking the wall, and freeze-dry to obtain Clostridium butyricum cell capsule material ;

[0030] (3) Butyric acid and Ca(OH) 2 The reaction generates a calcium butyrate solution, and the calcium butyrate solution is mixed with proteinase K to break the wall to obtain a wall breaking solution;

[0031] (4) mixing and stirring the wall-breaking liquid and the clostridium butyricum cell capsule material obtained in step (2), drying to obtain calcium butyrate microcapsules;

[0032] There is no chronol...

Embodiment 1

[0043] In the first step, Clostridium butyricum is inoculated into the seed medium for step-by-step amplified culture, and the obtained culture solution is centrifuged and washed to obtain the bacteria sludge;

[0044] Wherein, the primary medium composition (percentage by weight): 2% glucose, 1% yeast extract, 1% tuna extract, 1% peptone, 0.5% soluble starch, 0.5% sodium chloride, 0.05% glycerin, and the balance is water . Secondary medium composition (percentage by weight): 2.5% glucose, 2.0% corn steep liquor, 3.5% yeast extract, 1% beef extract, 0.2% ammonium sulfate, 0.5% bacterial protein, 0.2% potassium dihydrogen phosphate, Calcium chloride 0.2%, ammonium chloride 0.1%, magnesium sulfate 0.07%, manganese sulfate 0.02%, and the balance is water.

[0045] In the second step, NaCl solution was added to the prepared sludge to adjust the pH to 6.0, and proteinase K was added at the same time, and stirred at 55°C for 3 hours to break the wall; the broken bacteria were coole...

technical field

[0001] The invention belongs to the technical field of microcapsules, in particular to a calcium butyrate microcapsule and its preparation method and application. Background technique

[0002] Clostridium butyricum was first discovered and reported by Dr. Miyari Kinji of Chiba Medical University in Japan in 1933. Therefore, it is also known as Miyari bacteria. The classification belongs to the genus Clostridium and is a Gram-positive anaerobic bacillus. In 1935, Dr. Kingimiyairi of the Russian Institute of Microbiology isolated Clostridium butyricum from human feces and soil, and later found that its anaerobic cultured filtrate contained less fatty acids, which had a strong intestine-regulating effect, and it could inhibit The pathogenic bacteria in the intestinal tract can promote the growth of beneficial bacteria in the intestinal tract such as Bifidobacteria and Lactobacillus. In 1992, the Institute of Microbiology of the Heilongjiang Academy of Sciences i...

Examples

preparation example Construction

[0028] The invention provides a preparation method of calcium butyrate microcapsules, comprising the following steps: (1) inoculating Clostridium butyricum into a seed culture medium for cultivation, collecting the thalli in the culture solution, and obtaining the bacteria sludge after cleaning;

[0029] (2) Add NaCl solution and proteinase K to the bacterium slime, stir at 55-60°C for 1-3 hours, and then carry out wall-breaking treatment, collect the bacterium precipitate after breaking the wall, and freeze-dry to obtain Clostridium butyricum cell capsule material ;

[0030] (3) Butyric acid and Ca(OH) 2 The reaction generates a calcium butyrate solution, and the calcium butyrate solution is mixed with proteinase K to break the wall to obtain a wall breaking solution;

[0031] (4) mixing and stirring the wall-breaking liquid and the clostridium butyricum cell capsule material obtained in step (2), drying to obtain calcium butyrate microcapsules;

[0032] There is no chronol...

Embodiment 1

[0043] In the first step, Clostridium butyricum is inoculated into the seed medium for step-by-step amplified culture, and the obtained culture solution is centrifuged and washed to obtain the bacteria sludge;

[0044] Wherein, the primary medium composition (percentage by weight): 2% glucose, 1% yeast extract, 1% tuna extract, 1% peptone, 0.5% soluble starch, 0.5% sodium chloride, 0.05% glycerin, and the balance is water . Secondary medium composition (percentage by weight): 2.5% glucose, 2.0% corn steep liquor, 3.5% yeast extract, 1% beef extract, 0.2% ammonium sulfate, 0.5% bacterial protein, 0.2% potassium dihydrogen phosphate, Calcium chloride 0.2%, ammonium chloride 0.1%, magnesium sulfate 0.07%, manganese sulfate 0.02%, and the balance is water.

[0045] In the second step, NaCl solution was added to the prepared sludge to adjust the pH to 6.0, and proteinase K was added at the same time, and stirred at 55°C for 3 hours to break the wall; the broken bacteria were coole...