A kind of anti-infection silica biological tissue adhesive and its application

A technology of biological tissue and mesoporous silica, applied in the field of biomedicine, can solve the problems of toxic side effects of antibiotics, abuse, and limited antibacterial effect

Active Publication Date: 2022-06-07
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In addition, in most cases, trauma and infection always occur at the same time. Due to the impact of trauma environment and other factors, the incidence of post-injury infection is extremely high. At the same time, new secondary infection and cross-infection may be caused in the follow-up treatment
At present, the commonly used method to control bacterial infection is the application of antibiotics, but the antibacterial effect of antibiotics on local wounds is often limited, and there are also problems such as drug resistance, toxic side effects, and antibiotic abuse.

Method used

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  • A kind of anti-infection silica biological tissue adhesive and its application
  • A kind of anti-infection silica biological tissue adhesive and its application
  • A kind of anti-infection silica biological tissue adhesive and its application

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0042] Example 1 Preparation of mesoporous silica

[0043] Sample 1

[0044]Weigh 0.3g of CTAB into a 500mL three-neck flask, add 96mL of distilled water, protect the interior of the reaction device with nitrogen, stir magnetically at 70°C for 1 hour to fully dissolve it, weigh 45mL of n-octane, at a rate of 2 drops / second Drop into the above CTAB solution system. Continue to stir for 20 minutes after dripping to form a uniform emulsion. Measure 3.2 mL of tetraethyl orthosilicate TEOS dropwise into the above solution at a rate of 2 drops / sec. After dropping, 0.066 g of lysine was weighed and added to the above solution. Then, 8.9 mL of methyl methacrylate monomer was weighed and dropped into the above solution at a rate of 2 drops / sec. After dripping, 113.4 mg of azobisisobutyramidine hydrochloride AIBA was weighed and added to the reaction solution. After the addition was completed, the reaction was continued for 4 hours under magnetic stirring in a water bath at 70°C. ...

example 2

[0055] Example 2 Preparation of lysozyme-loaded mesoporous silica nanoparticles

[0056] 40 mg of lysozyme was weighed and placed in a 10 mL vial, 8 mL of PBS solution with pH 6.8 was added, and the lysozyme was fully dissolved by magnetic stirring at 200 rpm for 1 hour at 4°C. Then, 20 mg of the mesoporous silica nanoparticles prepared in Example 1 were weighed and placed in the above-mentioned lysozyme solution, and continued to be magnetically stirred at 200 rpm for 3 hours at 4°C. After stirring, the mixture was centrifuged at 8000 rpm for 3 minutes, and the supernatant was removed to obtain mesoporous silica nanoparticles loaded with lysozyme protein.

[0057] During the above preparation process of lysozyme-loaded mesoporous silica nanoparticles, samples were taken at 15 minutes, 30 minutes, 45 minutes, 1 hour, 1.5 hours, 2 hours, and 3 hours, respectively. The sampling method was as follows: 500 μL of the stirring solution was drawn and centrifuged at 8000 rpm for 3 mi...

example 3

[0060] Example 3 Drug release assay of lysozyme-loaded mesoporous silica nanoparticles

[0061] The remaining solution after loading lysozyme for 3 hours in Example 2 was centrifuged at 8000 rpm for 3 minutes, the solid precipitate was transferred to a 50 mL flat-bottomed flask, and 25 mL of pH 6.8 phosphate buffer was added. medicine for 24 hours. Samples were taken at 15 minutes, 30 minutes, 1 hour, 1.5 hours, 2 hours, 3 hours, 4 hours, and 5 hours, respectively. The sampling method is as follows: suck 1000μL of stirring solution and centrifuge at 8000rpm for 3 minutes, then take 500μL of supernatant and store it at 4°C for testing, add 500μL of PBS pH6.8 to the remaining liquid and solid, blow evenly, and add back to release the drug. system.

[0062] The 500 μL supernatant obtained at each time point was mixed with the Coomassie brilliant blue staining solution described in Example 2 and shaken up, and after 3 minutes of dark staining, the ultraviolet-visible absorbance ...

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Abstract

The invention relates to the preparation of a drug-loaded antibacterial nanometer mesoporous silicon dioxide biological tissue adhesive and its application in tissue adhesion. The preparation of the drug-loaded mesoporous silica includes the following steps: preparing an oil-in-water emulsion with n-octane, cetyltrimethylammonium bromide and water; The monomer is polymerized in the oil phase droplets, and the styrene is removed by demulsification, centrifugation and high-temperature calcination to obtain mesoporous silica nanoparticles; the prepared silica is added to the lysozyme solution to load the lysozyme, and the centrifugal drying Finally, the anti-infection silica biotissue adhesive is prepared. The invention aims to solve the disadvantages of secondary injury caused by surgical suture or limitations that certain organs and tissues are not suitable for suturing, poor effect of traditional organic bioadhesives and the like in wound treatment. It has the advantages of rapid action and no secondary trauma, and has broad application prospects in the field of wound treatment.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a preparation method and application of a novel biological adhesive, in particular to the preparation and application of an anti-infective nanometer mesoporous silica loaded with lysozyme. Background technique [0002] Injuries such as chronic intractable wounds and visceral surgical incisions place a heavy burden on patients and healthcare systems. Currently, surgical sutures and polymer adhesives are used as treatments for wounds caused by visceral surgery such as liver, lung, and heart. However, for soft tissues such as liver and lung, surgical suturing will cause certain damage to the organs, and will also leave incision gaps; polymer adhesives also have limited effects in the humid in vivo environment and control the polymerization or cross-linking in the body. The reaction conditions are too complicated and other reasons limit its application range. The study found that a drop of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L24/10A61L24/02A61L24/00
CPCA61L24/108A61L24/02A61L24/0015A61L24/001A61L2300/404A61L2400/12A61L2300/254A61L2300/602A61L2300/412Y02A50/30
Inventor 高亦鲲王思玲林伟康刘迎春
Owner SHENYANG PHARMA UNIVERSITY
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