Preparation method and use method of inorganic hybrid self-repairing hydrogel
A self-healing, hydrogel technology, used in pharmaceutical formulations, prostheses, drug delivery, etc., can solve the problems of uneven dispersion of manganese dioxide nanomaterials, poor tissue affinity of hydrogels, etc., and achieve efficient removal of active free base, good biocompatibility, and the effect of alleviating hypoxia
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[0026] Preparation method of inorganic hybrid self-healing hydrogel
[0027] Step 1: Dissolve tetramethylammonium hydroxide in 3% hydrogen peroxide aqueous solution, and quickly pour it into the divalent manganese salt solution, and keep stirring.
[0028] Step 2: Centrifuge the obtained black precipitate, wash and dry it with deionized water, and then use the method of ultrasonic mechanical exfoliation to obtain two-dimensional manganese dioxide nanosheets with a single-layer structure.
[0029] Step 3: mixing polylysine and manganese dioxide nanosheets to obtain polylysine-modified manganese dioxide composite nanosheets.
[0030] Step 4: Modification of phenolic groups to both ends of polyether F127, self-assembly in water to obtain F127 aldehyde micelles.
[0031] Step 5: Blend the polylysine-modified manganese dioxide composite nanosheets with the F127 modified with phenolic groups at both ends at 4°C, and form a gel at 37°C to obtain a hybrid self-healing hydrogel.
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Embodiment 1
[0044] Example 1: Preparation of two-dimensional manganese dioxide nanosheets modified by polylysine
[0045]Dissolve 0.378g manganese dichloride in 10ml deionized aqueous solution, weigh 2.2g tetramethylammonium hydroxide and dissolve it in 3% hydrogen peroxide solution, add it to the above manganese dichloride solution quickly, 600 Continue stirring per minute, react for 12 hours to obtain a black manganese dioxide precipitate, remove unreacted manganese dichloride and tetramethylammonium hydroxide with deionized water, and ultrasonically peel off for 30 minutes to obtain a monolayer structure of manganese dioxide. Dimension nanosheets, the resulting final product was freeze-dried. Disperse the above-mentioned manganese dioxide nanosheets into deionized water, prepare a 10 mg / ml solution, mix it with a 20 mg / ml polylysine solution, stir thoroughly for 12 hours, and wash it three times with deionized water after centrifugation to obtain The product was lyophilized.
Embodiment example 2
[0046] Example 2: Preparation of polyether F127 aldehyde modified with phenolic groups at both ends
[0047] Dry 12.6 g of polyether F127 with an average molecular weight of 12.6 kDa at 80° C. for 12 hours, add 120 ml of dichloromethane, place in an ice bath, and quickly add 0.875 ml of triethylamine. Under the protection of nitrogen atmosphere, 0.32 ml of methanesulfonyl chloride was dissolved in 20 ml of dichloromethane, and slowly added to the above mixture, and reacted at room temperature for 24 hours. After the reaction, 150 ml of deionized water was added, and extracted three times with 100 ml of dichloromethane. Wash the organic phase twice with 100 ml of 1M hydrochloric acid solution and saturated brine respectively, and then add anhydrous sodium sulfate to remove excess water. Ether was added to the concentrated mixture to precipitate the product, and after three purifications, the product was vacuum-dried. The obtained intermediate product, 0.74 g of p-hydroxybenza...
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