Supported nano zinc oxide as well as preparation and application thereof

A nano-zinc oxide, loaded technology, applied in the field of environmental pollution control, can solve the problems such as the rebound of antibiotic-resistant bacteria, achieve the effect of inhibiting photoremediation, simple preparation process, and convenient recycling

Active Publication Date: 2020-10-02
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a risk of photoreactivation after UV sterilization, that is, the number of antibiotic-resistant bacteria rebounded in the treated system

Method used

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  • Supported nano zinc oxide as well as preparation and application thereof
  • Supported nano zinc oxide as well as preparation and application thereof
  • Supported nano zinc oxide as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 ZnO@Al 2 o 3 Preparation and Characterization of Particles

[0037] Concrete preparation steps are:

[0038] S1. Prepare the template: 16g epoxy resin, 18g polyethylene glycol 1000 and 14g polyethylene glycol 2000 are vigorously stirred under heating conditions, and after they are all melted, 4g diethylenetriamine is quickly added, and quickly poured into polytetrafluoroethylene Curing reaction at 70°C for 3 hours in an ethylene square mold to generate an organic solid template with large pores, and drying at room temperature for 24 hours. Cut the template into about 1 cm × 1 cm × 1 cm block particles for Al 2 o 3 preparation.

[0039] S2. Preparation of Al by template method 2 o 3 Particles: 5.625g Al(NO 3 ) 3 After dissolving in 100 mL of deionized water, slowly add dropwise to 100 mL of 0.5 wt% chitosan in acetic acid solution, and add 5 g of the template particles obtained in S1. After the mixture was stirred for 1 h, aqueous ammonia was added dr...

Embodiment 2

[0052] Example 2 ZnO@Al 2 o 3 granular for sterilization

[0053] Experimental strain: Escherichia coli (RP4-E.coli) carrying RP4 plasmid was used as the sterilization object. Three ARGs including tetA, aphA and blaTEM-1 were encoded on the RP4 plasmid, which made E. coli resistant to 10mg / L tetracycline, 50mg / L kanamycin sulfate and 100mg / L ampicillin, respectively.

[0054] Take 0.1 mL of glycerol bacteria liquid thawed from -80°C refrigeration, inoculate it into 100 mL of selective LB liquid medium containing three kinds of antibiotics, and incubate at 37°C with shaking at 200 rpm. The nutritive substances in the LB liquid medium are 10g / LNaCl, 10g / L tryptone and 5g / L yeast extract; the selectivity of the LB liquid medium is aimed at 10mg / L tetracycline, 50mg / L kanamycin sulfate and 100mg / L ampicillin. The stable growth period was reached after 12 hours of cultivation, and the number of bacteria did not change basically. Therefore, the bacterial liquid after 12 hours of...

Embodiment 3

[0063] Embodiment 3UV-ZnO@Al 2 o 3 Inhibition of visible light reactivation repair of antibiotic-resistant bacteria

[0064] The experimental device is the same as in Example 2.

[0065] The non-viable bacteria system after UV irradiation, including UV irradiation alone and UV-ZnO@Al 2 o 3 The system is placed in the experimental device, and the whole device is also placed in a constant temperature incubator at 25° C., and the visible light irradiation (VL) button is turned on. After 0, 5, 10, 20, 40, 60, 120, 180, and 240 min of VL irradiation, take 0.1 mL of bacterial solution, dilute it with PBS buffer 10 times, and inoculate it on selective LB solid medium overnight. Incubate and count (CFU / mL). The selectivity of the LB solid medium is: 10mg / L tetracycline, 50mg / L kanamycin sulfate and 100mg / L ampicillin.

[0066] In this example, photoremediation experiments were carried out on bacterial liquids of different sterilization systems, in order to compare and obtain a s...

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Abstract

The invention provides supported nano-zinc oxide as well as a preparation method and application thereof. A carrier of the supported nano zinc oxide is aluminum oxide, wherein ZnO accounts for 9%-11%of the total mass; the particle size of the ZnO@Al2O3 particles is 3-5 [mu] m, and the surface of the ZnO-coated Al2O3 particles is needle-like zinc oxide with the length of 400-500 nm. The hydrothermal method is simple in process. The supported nano-zinc oxide has the effects of killing bacteria, inactivating antibiotic resistance genes and inhibiting repair under visible light. The supported nano-zinc oxide is convenient to recycle, agglomeration can be avoided, dispersion is facilitated, and more importantly, nano-particles cannot enter aquatic organisms to generate toxic action. ZnO and Al2O3 under ultraviolet irradiation are used as a sterilization system, and the sterilization temperature is 5-40 DEG C; wherein the ultraviolet irradiation time is 3 to 240 minutes, and the concentration of ZnO@Al2O3 is 10 to 250 mg / L. The effectiveness and durability of sterilization are improved. Meanwhile, a model is established for a mechanism of killing antibiotic-resistant bacteria and inactivating antibiotic-resistant genes by the supported nano-zinc oxide, so that theoretical support is provided for practical application of zinc oxide in the aspect of sterilization.

Description

technical field [0001] The invention belongs to the technical field of environmental pollution control, and in particular relates to a loaded nano zinc oxide and its preparation and application. Background technique [0002] Antibiotic resistance pollution caused by overuse of antibiotics has become an important environmental pollution problem. Antibiotic-resistant contamination includes antibiotic-resistant bacteria (ARBs) and resistance genes (ARGs). Currently, ARBs and ARGs have been found to be widely present in various environmental media, and antibiotic resistance contamination has been found even in many places where antibiotics were not detected. An important reason for the persistent and widespread distribution of antibiotic-resistant pollution is the vertical and horizontal transfer of ARGs. Therefore, how to effectively control antibiotic resistance pollution in the environment is an important environmental problem that needs to be solved at present. [0003] T...

Claims

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Application Information

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IPC IPC(8): B01J23/06B01J21/04B01J35/00B01J37/00B01J37/10C02F1/30C02F1/50A01N25/08A01N59/16A01P1/00
CPCB01J23/06B01J21/04B01J37/0018B01J37/10C02F1/30C02F1/50A01N25/08A01N59/16C02F2305/10C02F2303/04B01J35/39
Inventor 郭平王旭贾晓慧左闻达申艳萍刘亦博程成袁伟琳
Owner JILIN UNIV
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