Unlock instant, AI-driven research and patent intelligence for your innovation.

Casein purification method and preparation method of iron-protein succinylate

A technology of iron protein succinate and purification method, which is applied in the preparation methods of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problem of low iron absorption rate, nausea, vomiting, preparation method and characterization research of iron protein succinate. less problem

Active Publication Date: 2020-12-11
杭州汉库医药科技有限公司
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The second-generation iron supplement solves the problem of low iron absorption rate and generally improves iron absorption, but there are still shortcomings, such as ferrous lactate has a strong iron taste, and can cause nausea, vomiting, upper abdominal pain, etc. Severe gastrointestinal adverse reactions; ferrous gluconate has astringent taste and caramel smell, and the side effects are similar to ferrous lactate, and other second-generation iron supplements also have similar adverse reactions
The cost of third-generation iron supplements is relatively high and cannot be popularized now. Although the absorption rate of iron has been improved, it is still not satisfactory
But this patent only considers that the amount of said protein impurity is below about 15%, preferably below about 10%. The protein impurity is reduced through physical purification, but it cannot completely eliminate the impurity or reduce the impurity content to an extremely low level.
[0013] Iron protein succinate oral solution is currently on the market in China, but the relevant patents are mostly focused on the development of preparations, and there are few studies on the preparation method and characterization of iron protein succinate, and the knowledge of its microstructure is negligible

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Casein purification method and preparation method of iron-protein succinylate
  • Casein purification method and preparation method of iron-protein succinylate
  • Casein purification method and preparation method of iron-protein succinylate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Extraction and purification of embodiment 1 casein

[0071] (1) Take an appropriate amount of milk, cool it to about 4°C, centrifuge at 3000r / min for 10min, and remove the upper fat layer;

[0072] (2) Put the lower emulsion in a beaker, heat it to about 40°C, slowly add an appropriate amount of hydrochloric acid under stirring, adjust the pH to about 4.7, and produce a large amount of flocculents. Cool to room temperature, centrifuge at 3000r / min for 10min, and discard the supernatant.

[0073] (3) The precipitate was washed three times with purified water, centrifuged at 3000r / min for 10min, and the supernatant was discarded to obtain crude casein.

[0074] (4) Mash the sediment, add an appropriate amount of ethanol, stir and let it stand for a while, transfer the suspension to the Buchner funnel for suction filtration; continue to mash the filter cake, add an appropriate amount of ether, stir and let it stand for a while, transfer the suspension to Buchner funnel s...

Embodiment 2

[0078] The preparation of embodiment 2 iron protein succinate

[0079] (1) Weigh a certain amount of casein, add an appropriate amount of sodium carbonate solution to dissolve it.

[0080] (2) After the protein is completely dissolved, heat to 45°C, add an appropriate amount of succinic anhydride, add an appropriate amount of sodium carbonate solution and maintain the pH at about 8.0, and after acylation for 2~3 hours (the degree of acylation is 90%), succinic acid is obtained Crude protein.

[0081] (3) Add hydrochloric acid to the crude protein succinic acid solution to acidify slowly to pH 2.2, and a white precipitate is precipitated, which is separated by filtration; the precipitate is washed with water and redissolved in sodium carbonate solution, then acidified to pH 2.2 by adding hydrochloric acid and Precipitation of white precipitate; repeated several times until obtaining refined succinic acid protein.

[0082] (4) Add an appropriate amount of sodium carbonate solu...

Embodiment 3

[0086] The release medium of embodiment 3 iron protein succinate

[0087] According to the special properties of this product, the release rate of this product is an important criterion to distinguish the quality of the product. The iron release of iron protein succinate in different media was investigated (see attached figure 1 ), dissolution conditions: paddle method, 50rpm, 900ml medium.

[0088] Among them, the blue curve uses hydrochloric acid solution (pH1.2) for 0~2h, and phosphate solution (pH6.8) for 2~4h. It can be found that iron protein succinate is almost not released under this condition.

[0089] Changing the investigation conditions, the red curve uses artificial gastric juice for 0~2h, and artificial intestinal juice for 2~4h. It can be found that iron protein succinate is almost not released in artificial gastric juice, but is released rapidly in artificial intestinal juice. Therefore, artificial intestinal juice can be used as the release medium of iron pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle sizeaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of biological medicines, and particularly relates to a casein purification method and a preparation method of iron-protein succinylate. The casein purification method disclosed by the invention comprises the following steps: 1) properly hydrolyzing casein by adopting acid protease or neutral protease; 2) hydrolyzing impurity protein and polypeptide by adopting amode of combining aminopeptidase and carboxypeptidase; and 3) removing amino acids and oligopeptide generated after hydrolysis according to an isoelectric precipitation method, and controlling the molecular weight of amino acids and oligopeptide with bitter taste. The casein purified by the method is high in utilization rate and low in bitterness, gastrointestinal discomfort is relieved, and ironcan be quickly released. Besides, the purified casein is applied to a preparation method of the iron-protein succinylate, succinyl protein is obtained through acylation of the purified casein, and a proper succinyl protein and ferric iron combined complexing constant K is obtained by controlling the proper acylation degree, so that the purposes of releasing the effective dosage of iron and controlling the cost are achieved.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for purifying casein and a method for preparing iron protein succinate. Background technique [0002] Iron deficiency is a widespread worldwide nutritional deficiency that seriously threatens human health. Normal people need to take in 1-1.5mg of iron from the outside world every day to maintain the balance of iron metabolism in the body. The daily intake of iron for pregnant and lactating women Need to reach 2 ~ 4mg, so pregnant women and lactating women are more likely to cause insufficient iron intake. [0003] The most representative iron-deficiency disease is iron-deficiency anemia. According to the World Health Organization (WHO), one-third of the world's people suffer from iron-deficiency anemia, regardless of developed or developing countries. The proportion is very high. According to statistics, the incidence rate of iron deficiency anemia is 10% in men,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/47C07K1/36C07K1/30C07K1/24C07K1/107A61P3/02
CPCA61P3/02C07K14/4732C12P21/06
Inventor 孙军陆慧沈子良吴佳佳寿宇捷
Owner 杭州汉库医药科技有限公司