Extraction method and application of Korla pear acidic polysaccharide

A technology of Korla fragrant pear and acidic polysaccharides, which is applied in the field of extraction of Korla fragrant pear acidic polysaccharides, can solve problems such as inflammation and achieve good activity, high extraction rate, and mild conditions

Active Publication Date: 2021-01-15
XINJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The traditional method of improving the immune regulation activity mediated by macrophages is mainly to intervene with lipopolysaccharide, but long-term and large-scale use of lipopolysaccharide can easily induce inflammatory reactions

Method used

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  • Extraction method and application of Korla pear acidic polysaccharide
  • Extraction method and application of Korla pear acidic polysaccharide
  • Extraction method and application of Korla pear acidic polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The extraction method of the Korla fragrant pear acidic polysaccharide of the present embodiment, the method is:

[0034] S1. Cut the Korla fragrant pear fruit into shreds after removing the core, place it in a cool and ventilated place for 1 month to dry, obtain the dried pulp, soak the dried pulp in an aqueous ethanol solution with a mass fraction of 95% to degrease Take it out after 8 hours to obtain degreased pulp, add distilled water a to the degreased pulp, then reflux and extract at a temperature of 90°C, and then filter to obtain filtrate a, add distilled water b to the filtered pulp residue a, Reflux and extract at a temperature of 90°C and filter to obtain filtrate b, add distilled water c to the filtered pulp residue b, reflux and extract at a temperature of 90°C and filter to obtain filtrate c, and combine filtrates a, Filtrate b and filtrate c to obtain extracts; reflux extraction time is 2h; the dosage ratio of degreased pulp and distilled water a is 1g: 4...

Embodiment 2

[0047] This example provides the application of two kinds of Korla fragrant pear acidic polysaccharides prepared in Example 1. The Korla fragrant pear acidic polysaccharides PSAP-1 and PSAP-2 are used to reduce airway inflammation of macrophages, neutrophils, The number of eosinophils and lymphocytes can reduce the content of IL-4, IL-5 and IL-13, and repair the damage of lung tissue.

[0048] Take 72 normal female BALB / c mice, body weight 18±5g, and divide them into 9 groups randomly, 8 mice in each group, normal control group, asthma model group, PSAP-1 low dose group (10mg / kg), PSAP-1 Medium-dose group (20mg / kg), PSAP-1 high-dose group (40mg / kg), PSAP-2 low-dose group (10mg / kg), PSAP-2 medium-dose group (20mg / kg), PSAP-2 high-dose group group (40mg / kg), dexamethasone positive control group. Except for the normal control group, the other 8 groups were sensitized by intraperitoneal injection of 0.2 mL of antigen solution (containing 100 μg of OVA and 200 μg of aluminum hydro...

Embodiment 3

[0066] This example provides the application of two kinds of Korla fragrant pear acidic polysaccharides prepared in Example 1. The Korla fragrant pear acidic polysaccharides PSAP-1 and PSAP-2 are used to improve the proliferation activity of RAW264.7 macrophages and promote RAW264. 7 The release of NO from macrophages can promote the secretion of cytokine IL-6 and cytokine TNF-α by RAW264.7 macrophages, and improve the immune regulation activity mediated by macrophages.

[0067] (1) Cell proliferation activity

[0068] Take the RAW264.7 macrophages in the logarithmic growth phase, and use 1×10 5 The density of cells / mL was inoculated in 96-well plates, and cultured in a carbon dioxide incubator for 24 hours until the cells adhered to the wall. After the supernatant was discarded, each concentration of PSAP-1 and PSAP-2 (3.9, 7.8, 15.6, 31.3, 62.5, 125, 250, 500, 1000, 2000 μg·mL -1 ) complete cell culture medium for cell intervention, and set a lipopolysaccharide positive co...

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Abstract

The invention provides an extraction method of Korla pear acidic polysaccharide, which comprises the following steps of denucleating Korla pear fruits, shredding, airing, degreasing, carrying out reflux extraction to obtain an extracting solution, evaporating, concentrating, adding an ethanol water solution, collecting the precipitate, dissolving with distilled water, dialyzing, concentrating, andcarrying out vacuum freeze drying to obtain the Korla ear polysaccharide. After three times of elution, two kinds of Korla pear acidic polysaccharides PSAP-1 and PSAP-2 are obtained. The invention further provides application of the PSAP-1 and the PSAP-2, and the PSAP-1 and the PSAP-2 are used for reducing the number of macrophages, neutrophils, eosinophils and lymphocytes of the airway inflammation, reducing the content of IL-4, IL-5 and IL-13 and repairing damage to lung tissue. The method is used for improving the proliferation activity of RAW264.7 macrophages, promoting NO release of theRAW264.7 macrophages, promoting the RAW264.7 macrophages to secrete cytokine IL-6 and cytokine TNF-alpha, and improving the macrophage mediated immunomodulatory activity.

Description

technical field [0001] The invention belongs to the technical field of Korla fragrant pear acidic polysaccharide, and in particular relates to an extraction method and application of the Korla fragrant pear acidic polysaccharide. Background technique [0002] The extraction methods of plant polysaccharides mainly include acid-base extraction, biological enzyme extraction, ultra-high pressure extraction and microwave extraction. The acid-base extraction method is easy to break the glycosidic bond, the cost of the biological enzyme extraction method is high, the ultra-high pressure extraction method is easy to destroy the internal structure of the polysaccharide, and the microwave extraction method has high requirements for equipment. [0003] The separation methods of plant polysaccharides mainly include: fractional precipitation, quaternary ammonium salt precipitation, electrophoresis and metal ion precipitation. The fractional precipitation method can only be used for the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K36/73A61K31/715A61P37/02A61P37/04A61P11/00
CPCC08B37/0003A61K36/73A61K31/715A61P37/02A61P37/04A61P11/00
Inventor 海力茜·陶尔大洪董彩霞周凯马晓丽杨飞陈春丽庞国凤汪芳聂昌宏热孜亚·吾甫尔李亚童李欢欢乔丽洁阿吉然·阿布拉米合热尼沙·阿木热江卡地尔亚·库尔班
Owner XINJIANG MEDICAL UNIV
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