Composite microbial preparation for fermented feed and fermented expanded pellet feed

A technology of extruded pellet feed and compound microorganisms, applied in animal feed, animal feed, application, etc., can solve the problems of poor probiotic effect and easy inactivation of probiotics, and achieve strong degradation ability, good feeding effect, and acid production capacity strong effect

Pending Publication Date: 2021-02-05
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to pro

Method used

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  • Composite microbial preparation for fermented feed and fermented expanded pellet feed
  • Composite microbial preparation for fermented feed and fermented expanded pellet feed
  • Composite microbial preparation for fermented feed and fermented expanded pellet feed

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030]Example 1: Isolation, screening and gene sequencing of Lactobacillus salivarius LS strain

[0031]Source of strain: healthy intestinal contents of grass carp (aquaculture base of Jiangxi Agricultural University).

[0032]Method: Dissecting healthy grass carp in an ultra-clean workbench, take the contents of the intestinal tract and directly dilute it to 10 times in sterile normal saline.-2-10-8Concentration, take 0.1ml and spread it on MRS solid medium containing 1% calcium carbonate, and incubate at 30°C for 24-48h. Pick a single colony that produces a calcium lysis ring from the MRS solid medium containing 1% calcium carbonate and purify it on the ordinary MRS solid medium. After purification twice, perform Gram staining and biochemical identification. The single colony grown on MRS The colonies were inoculated in the corresponding liquid medium and cultured at 30°C and 180r / min for 24-48h.

[0033]Spot a single strain in the MRS liquid medium on the MRS solid medium with 1% calcium ...

Example Embodiment

[0038]Example 2: Isolation, screening and gene sequencing of Bacillus subtilis B1 strain

[0039]Source of strains: termite intestinal material (termites are collected from the campus woods of Jiangxi Agricultural University); healthy grass carp (aquaculture base of Jiangxi Agricultural University) intestinal contents; one strain of Bacillus subtilis and one strain of Bacillus amyloliquefaciens , The two strains have antibacterial effect on Aeromonas hydrophila, and they are separated and preserved by our laboratory.

[0040]Indicator bacteria: Aeromonas hydrophila AH-S1, Aeromonas sobria AS-S2, isolated from the liver of diseased rice field eel, preserved by our laboratory.

[0041]Medium:

[0042]Guaiacol primary screening medium: yeast extract 10g / L, glucose 20g / L, guaiacol 2.5g / L, agar 15g / L, adjust pH to 7.0, autoclave at 121°C for 20 min.

[0043]RB brilliant blue medium: RB brilliant blue 0.03g / L, yeast extract 5g / L, tryptone 10g / L, sodium chloride 10g / L, adjust pH to 7.0, autoclave at 121°...

Example Embodiment

[0061]Example 3: Optimization of the compound ratio of three strains in the compound microbial preparation

[0062]Compound microbial preparation:

[0063]A Bacillus subtilis B1 strain capable of degrading lignin, isolated from the intestinal material of termites, obtained through isolation and screening in Example 2, and the deposit number is CGMCC NO.20405;

[0064]A Lactobacillus salivarius (Lactobacillus salivarius) LS strain was isolated from the intestinal contents of healthy grass carp, obtained by isolation and screening in Example 1, and the deposit number is CGMCC NO.14458;

[0065]A strain of Saccharomyces cerevisiae J1 was purchased from the General Microbiology Center of China Microbial Species Collection Management Committee, and the preservation number is CGMCC NO.2.388.

[0066]Feed formula:

[0067]Feed raw materials and processing pellets are provided by Dayunong Biotechnology Co., Ltd. The test feed is an extruded pellet feed with a particle size of 1.0-1.2mm. The composition of th...

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Abstract

The invention relates to the technical field of fermented feeds, and particularly discloses a composite microbial preparation for a fermented feed and a fermented expanded pellet feed. The composite microbial preparation consists of three bacterial liquid composite proportions of a self-screened and preserved bacillus subtilis B1 strain with the preservation number of CGMCC NO.20405, a self-screened and preserved lactobacillus salivarius LS strain with the preservation number of CGMCC NO.14458 and a saccharomyces cerevisiae J1 strain with the preservation number of CGMCC NO.2.388 purchased from a preservation center. The composite microbial preparation is used for preparing the fermented expanded pellet feed. According to the fermented expanded pellet feed, a preparation process of firstlyprocessing and granulating the feed, then carrying out composite fermentation by using the composite microbial preparation and not needing to process and granulate again after fermentation is adopted, the inactivation of viable bacteria is avoided, and the loss of nutrient substances in the processing process is also reduced. The feed has the characteristics of good metabolite, sufficient nutritional value, high viable organism content, good feeding effect and the like.

Description

technical field [0001] The invention belongs to the technical field of fermented feed, in particular to a compound microbial preparation for fermented feed and fermented puffed granular feed. Background technique [0002] Probiotics generally refer to a type of live bacteria that can survive in the intestinal tract of animals and are beneficial to animal health. With the advent of the "antibiotic-free" era, the use of probiotics is becoming more and more widespread. Probiotics can synthesize and secrete some amino acids and vitamins needed by animals in the intestinal tract of the host, and can also secrete some proteases, cellulose, etc. Enzymes and amylases can promote the digestion and absorption of feed by animals, improve feed utilization and reduce feed coefficient; they can also improve the body's immunity and maintain the balance of intestinal flora structure. Among them, Bacillus sp., lactobacillus and saccharomyce are several micro-ecological strains widely used i...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K10/12A23K10/22A23K10/37A23K10/30A23K10/20A23K20/158A23K20/26A23K20/10A23K20/147A23K50/80A23K50/75
CPCA23K10/18A23K10/12A23K10/22A23K10/37A23K10/30A23K10/20A23K20/158A23K20/26A23K20/10A23K20/147A23K50/80A23K50/75Y02P60/87Y02A40/818
Inventor 张锦华周秋白王颖
Owner JIANGXI AGRICULTURAL UNIVERSITY
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