Application of marine-derived phospholipid on aspect of accelerating angiogenesis

An angiogenesis and phospholipid technology, applied in the field of biomedicine, can solve the problems of large structural differences and different sources of phospholipids, and achieve the effects of increasing the number of production, less toxic and side effects, and broad market prospects

Active Publication Date: 2021-02-05
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the sources of phospholipids are different, even the same type of phospholipids have different fatty acid side chain lengths, the number and position of unsaturated bonds, and the structure is quite different

Method used

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  • Application of marine-derived phospholipid on aspect of accelerating angiogenesis
  • Application of marine-derived phospholipid on aspect of accelerating angiogenesis
  • Application of marine-derived phospholipid on aspect of accelerating angiogenesis

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0058] Determination of phospholipid content

[0059] The invention adopts spectrophotometry (molybdenum blue colorimetric method) to measure the content of phospholipids. The standard curve equation of the potassium dihydrogen phosphate made is: y=0.8458x+0.0057 (R 2 = 0.9998).

[0060] Accurately measure 0.3mL of phospholipid sample solution (using methylene chloride as solvent, dissolve the phospholipid sample in methylene chloride to obtain a phospholipid sample solution with a mass concentration of 0.5mg / mL) and place it in a 10mL graduated test tube (blank control Measure 0.3mL of dichloromethane solvent), evaporate the solvent in a water bath, add 4 drops of concentrated sulfuric acid and 3 drops of perchloric acid to digest on the electric furnace until it is colorless and clear, replenish water to 2mL after cooling, add 1 drop of phenolphthalein indicator, and use 50 Neutralize the solution with % sodium hydroxide until the solution is red, slowly add dilute sulfuri...

experiment example 2

[0062] The UPLC-Q-Exactive Orbitrap / MS method was used to determine the composition and relative content of various phospholipids in marine phospholipid samples. The specific references for the calculation method of the relative content are: Xiaobin Li, et al. Lipidfingerprinting of different material sources by UPLC- Q-Exactive Orbitrap / MS approach and their zebrafish-based activities comparison, Journal of Agricultural and Food Chemistry, 2020, 68, 2007-2015.

Embodiment 1

[0064] Promotion of Antarctic krill phospholipids on the generation of ISVs in zebrafish

[0065] (1) Preparation of Antarctic krill phospholipids

[0066] Weigh 100 g of Antarctic krill that has been frozen for 30 days, pulverize, homogenize, add 800 mL of ethanol-water solution with a concentration of 90%, stir and extract at a constant speed for 2 times, each time for 6 hours, combine the extracts, and filter under reduced pressure. Concentrate under reduced pressure at 45°C to obtain crude phospholipids. Crude phospholipids were dissolved in 60% ethanol-water solution as a solvent, the volume of the solvent was 200 mL, after standing for 24 hours, centrifuged for 15 minutes, and the precipitated part was retained. Make a 0.5g / mL solution with n-hexane, add 56mL of acetone, and let it stand in a refrigerator at 4°C for 24h to precipitate. Centrifugal 15min removes supernatant, and final insoluble matter is vacuum-dried, obtains Antarctic krill phospholipid, and its yield ...

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Abstract

The invention relates to application of marine-derived phospholipid on an aspect of accelerating angiogenesis, and belongs to the technical field of biological medicines. Marine phospholipid is applied to a zebra fish model so as to evaluate an influence of the marine phospholipid on zebra fish body intersegmental vessel (ISVs) injuries induced by vascular endothelial cell growth factor receptor inhibitor (PTK787) and a zebra fish subintestinal vein vessel (SIVs) growth situation. The invention discovers for the first time that the marine-derived phospholipid can reverse the zebra fish ISVs injuries induced by the PTK787, has an obvious acceleration function for zebra fish SIVs growth, can obviously increase the generation amount and the blood vessel length of blood vessels, and has a small toxic or side effect. The marine-derived phospholipid can provide a candidate for preparing medicines used for treating ischemic diseases, and has a good application prospect.

Description

technical field [0001] The invention relates to the application of marine-sourced phospholipids in promoting angiogenesis, and belongs to the technical field of biomedicine. Background technique [0002] Angiogenesis refers to the process of sprouting new vasculature based on the original vascular plexus or existing blood vessels, and it is an essential mechanism to maintain the function of the circulatory system and ensure energy supply. Many important human diseases, such as cancer, cardiovascular and cerebrovascular diseases, and complications of diabetes, are closely related to angiogenesis. When angiogenesis is insufficient, it will cause myocardial ischemia, cerebral ischemia, slow healing of trauma and fracture, vasculitis obliterans, diabetic peripheral vascular disease and other diseases. Finding therapeutic drugs that can promote angiogenesis is an important strategy for the treatment of these ischemic diseases, which is also a hot spot in clinical research today....

Claims

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Application Information

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IPC IPC(8): A61K35/612A61K35/618A61K35/60A61K31/683A61K31/685A61K31/688C07F9/10C07F9/117C07F9/09A61P9/00A61P3/02A23L33/115A23L33/10
CPCA61K35/612A61K35/618A61K35/60A61K31/683A61K31/685A61K31/688C07F9/103C07F9/117C07F9/091A61P9/00A61P3/02A23L33/115A23L33/10A23V2002/00A23V2250/2042A23V2250/1842A23V2250/185A23V2250/1848A23V2250/1846A23V2200/326Y02A40/81
Inventor 李晓彬张姗姗张轩铭王利振刘可春张梦启李培海侯海荣
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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