Composition and application of Podocarpus macrophyllus fruit polysaccharide

A technology of polysaccharides and arhats, which is applied in the fields of medical technology and health food, and can solve unpredictable problems

Inactive Publication Date: 2021-02-09
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, for Podocarpus polysaccharide, whether it has the function of immune regulation and immunity enhancement, and how effective it is, professionals in the field cannot predict

Method used

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  • Composition and application of Podocarpus macrophyllus fruit polysaccharide
  • Composition and application of Podocarpus macrophyllus fruit polysaccharide
  • Composition and application of Podocarpus macrophyllus fruit polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Determination of Molecular Weight of Podocarpus Polysaccharide

[0025] Gel Permeation Chromatography (GPC) method was used to determine the molecular weight of podocarpus polysaccharide.

[0026] Dihydrogen potassium phosphate (KH 2 PO 4 ) The mobile phase was prepared into 2.0 mg / ml standard solution and sample solution to be tested, filtered with a 0.45 μm filter membrane, and injected for detection respectively. Chromatographic conditions: Chromatographic column: TSK G-5000 PWXL column (7.8×300mm) and TSK G-3000 PWXL column (7.8×300mm) in series; mobile phase: 0.02mol / L KH 2 PO 4 Buffer solution; flow rate: 0.6mL / min; injection volume: 10μL; Waters2414 differential detector; column temperature: 35°C; measurement time: 35min. Record the spectrum. The results were analyzed with Breeze GPC software, and the relative molecular mass fraction (logMw) of the dextran standard was taken as the ordinate, and the elution volume (V) was taken as the abscissa to create a st...

Embodiment 2

[0030] Composition Analysis of Podocarpus Polysaccharide

[0031] Determination of monosaccharide composition: Determination by PMP pre-column derivatization-HPLC method.

[0032] Accurately weigh 10 mg of Podocarpus polysaccharide into a 20 mL crimp-top bottle, add 5 mL of 2 mol / L trifluoroacetic acid (TFA), and fill the tube with nitrogen gas (10 L / min, 1 min). Hydrolyze in an oven at 100°C for 120 minutes; add 1mL of methanol after cooling, place in a water bath at 70°C, blow dry with nitrogen, repeat the operation more than two times to completely remove TFA; dissolve the product of the previous step with 1mL 0.3mol / L NaOH solution , polysaccharide hydrolyzate.

[0033] Precisely measure 400 μL of mixed monosaccharide standard solution and polysaccharide hydrolyzate in a 5 mL stoppered test tube, then add 400 μL PMP methanol, mix thoroughly, react in a water bath at 70 °C for 120 min, then take it out and cool it down to room temperature; add 400 μL of 0.3 mol / L of HCl ...

Embodiment 3

[0036] NMR Spectrum Analysis of Podocarpus Polysaccharide

[0037] Get 40 mg of Podocarpus polysaccharide after vacuum drying, dissolve it with deuterated dimethyl sulfoxide (DMSO), transfer it to an NMR tube, and detect it with a nuclear magnetic resonance spectrometer to obtain the 1H-NMR spectrum and 13C of Podocarpus polysaccharide. - NMR spectrum.

[0038] Figure 4 with 5 They are 1H-NMR spectrum and 13C-NMR spectrum respectively. Figure 4 It shows that there is a proton signal peak at 5.36ppm, and there are abundant proton signal peaks at less than 5.0ppm, indicating that Podocarpus polysaccharide mainly contains β-type glycosidic bonds and a small amount of α-type glycosidic bonds. The proton resonance peak appears in a very small region of 3.2ppm-3.65ppm. Among them, the signal peak at δ5.36 is the anomeric hydrogen of α-D-mannose, δ4.76 is attributed to the anomeric hydrogen of β-D-glucose, and the signal peak of β-D-galactose anomeric hydrogen is at δ4.67. str...

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Abstract

The invention relates to composition of podocarpus macrophyllus fruit polysaccharide and application of the podocarpus macrophyllus polysaccharide in the aspect of immunomodulatory function. The podocarpus macrophyllus fruit polysaccharide is polysaccharide obtained by separating and purifying from receptacles of podocarpus macrophyllus or podocarpus brevis. The weight-average molecular weight ofthe polysaccharide is 6018Da, and the number-average molecular weight of the polysaccharide is 6690Da. The polysaccharide belongs to heteropolysaccharide and is composed of mannose, glucose and galactose (the molar ratio is 21.26: 73.96: 4.78). Tthe connection mode is glycosidic bonds with alpha- and beta- configurations. The podocarpus macrophyllus fruit polysaccharide disclosed by the inventioncan promote proliferation of T and B lymphocytes in splenocytes of mice, so that cellular immunity and humoral immune response of organisms are promoted, and the immunity of the organisms is improved;by regulating the expression of serum cytokines IL-2, IL-6, TNF-alpha and INF-gamma, the immune function of an organism can be improved. The podocarpus macrophyllus fruit polysaccharide can be used for preparing food or preparing health food or medicine for enhancing immunity.

Description

technical field [0001] The invention belongs to the fields of medical technology and health food, and in particular relates to the composition of podocarpus polysaccharide and its use for enhancing immunity. Background technique [0002] Podocarpus polysaccharide is a kind of Podocarpus macrophyllus (Thunb.) D.Don or Podocarpus macrophyllus (Thunb.) D.Don var.maki (Sieb.) Endl. Natural active polysaccharide. It has been reported in the literature that the content of podocarpus fruit polysaccharide in fresh Podocarpus receptacle is about 0.5%. Most polysaccharides derived from plants have good biological activities. The reported biological activities include: immune regulation, anti-tumor, hypolipidemic, anti-oxidation, treatment of atherosclerosis, treatment of Alzheimer's disease, etc. For example, it has been reported in the literature that Lentinan has anti-tumor effect; Rosa laevigata polysaccharide has anti-viral effect; Rubus xylopolysaccharide has immunomodulatory a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23L33/125A23L2/38A23G3/42A61P37/04
CPCC08B37/006C08B37/0003A23L33/125A23L2/38A23G3/42A61P37/04A23V2002/00A23V2200/324A23V2250/51
Inventor 黄增琼曾凡艳林乐珍
Owner GUANGXI MEDICAL UNIVERSITY
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